| 1 | Lake Erie College of Osteopathic Medicine INC | Education | $222.1M | $1.1B |
| 2 | Millcreek Community Hospital | Health Care | $186.9M | $131.4M |
| 3 | Gannon University | Education | $174.8M | $324.8M |
| 4 | Mercyhurst University | Education | $164.8M | $161.6M |
| 5 | Erie Community Foundation | Philanthropy, Voluntarism & Grantmaking | $154.2M | $385.7M |
| 6 | Northwestern Region Employee Norebt | Unclassified | $146.2M | $17.7M |
| 7 | Dr Gertrude a Barber Center INC | Human Services | $101.0M | $55.7M |
| 8 | Millcreek Manor | Health Care | $53.1M | $95.2M |
| 9 | Northwest Institute of Research INC | Unclassified | $39.5M | $7.4M |
| 10 | Erie Homes for Children and Adults INC | Mental Health & Crisis Intervention | $33.4M | $16.1M |
| 11 | Child Development Centers INC | Unclassified | $31.9M | $25.8M |
| 12 | Erie County Care Managementincorporated | Mental Health & Crisis Intervention | $27.3M | $18.6M |
| 13 | Lakeshore Community Services INC | Human Services | $25.4M | $14.5M |
| 14 | Emergycare INC | Health Care | $25.1M | $14.8M |
| 15 | Dr Gertrude a Barber in Home Services INC | Human Services | $24.5M | $5.8M |
| 16 | Community Resources for Independence INC | Unclassified | $24.3M | $33.2M |
| 17 | Loyal Christian Benefit Association | Unclassified | $22.9M | $125.5M |
| 18 | Second Harvest Food Bank of Northwest Pennsylvania | Food, Agriculture & Nutrition | $22.6M | $14.0M |
| 19 | Stairways Behavioral Health | Mental Health & Crisis Intervention | $22.4M | $13.5M |
| 20 | Sarah a Reed Home | Unclassified | $21.6M | $18.4M |
| 21 | Sarah a Reed Retirement Center | Human Services | $20.0M | $38.0M |
| 22 | Voices for Independence | Education | $18.1M | $11.8M |
| 23 | Young Mens Christian Association of Erie Pa | Human Services | $18.1M | $28.2M |
| 24 | Saint Marys Home of Erie | Health Care | $17.6M | $47.6M |
| 25 | Achievement Center INC | Mental Health & Crisis Intervention | $16.9M | $14.7M |
| 26 | Presbyterian Homes in the Presbytery of Lake Erie | Unclassified | $15.7M | $19.5M |
| 27 | Greater Erie Community Action Committee | Human Services | $15.6M | $14.4M |
| 28 | Erie Cemetery | Mutual & Membership Benefit | $14.3M | $39.9M |
| 29 | Perseus House Charter School of Excellence | Education | $13.8M | $8.3M |
| 30 | Family Services of Nw Pa | Human Services | $13.7M | $5.8M |
| 31 | Perseus House INC | Human Services | $12.9M | $9.7M |
| 32 | Montessori Regional Charter School | Education | $12.6M | $17.3M |
| 33 | United Way of Erie County | Philanthropy, Voluntarism & Grantmaking | $12.5M | $37.9M |
| 34 | Community Health Net | Health Care | $11.9M | $9.3M |
| 35 | Historic Erie Preservation Trust | Philanthropy, Voluntarism & Grantmaking | $11.5M | $22.7M |
| 36 | Lecom at Presque Isle INC | Housing & Shelter | $11.3M | $20.5M |
| 37 | Ball Pavilion | Human Services | $11.1M | $10.1M |
| 38 | Worldserve International | International, Foreign Affairs & National Security | $11.0M | $4.2M |
| 39 | Medical Associates of Erie | Health Care | $9.9M | $10.5M |
| 40 | Visiting Nurse Association of Erie County | Unclassified | $9.7M | $9.4M |
| 41 | Supportive Living Services INC | Human Services | $9.6M | $11.3M |
| 42 | Robert Benjamin Wiley Community Charter School | Education | $9.5M | $7.2M |
| 43 | Service Coordination Unlimited INC | Diseases, Disorders & Medical Disciplines | $9.2M | $5.6M |
| 44 | Enterprise Development Center of Erie County INC | Community Improvement & Capacity Building | $8.6M | $24.2M |
| 45 | Kahkwa Club | Unclassified | $8.4M | $13.8M |
| 46 | Larche Erie INC | Unclassified | $8.2M | $7.1M |
| 47 | The Lutheran Foundation for Long- Term Living | Philanthropy, Voluntarism & Grantmaking | $8.2M | $126.6M |
| 48 | Community Blood Bank of Erie County | Health Care | $8.1M | $5.2M |
| 49 | Corry Community Foundation | Community Improvement & Capacity Building | $8.0M | $19.5M |
| 50 | Lecom Health Risk Retention Group | Health Care | $7.6M | $23.8M |
| 51 | IBEW Welfare Fund 56 | Unclassified | $7.4M | $9.9M |
| 52 | A Bridge to Independence | Human Services | $7.4M | $1.5M |
| 53 | Millcreek Health System Medical Professional Liability Self-ins Tr | Health Care | $7.1M | $20.0M |
| 54 | Manufacturer & Business Association | Unclassified | $7.1M | $15.6M |
| 55 | Jem Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $7.0M | $26.1M |
| 56 | Multi-cultural Health Evaluation Delivery Systems INC | Health Care | $6.9M | $4.2M |
| 57 | United Electrical Radio & Machine Workers of America | Unclassified | $6.4M | $14.2M |
| 58 | Erie Zoological Society | Animal-Related | $5.7M | $15.7M |
| 59 | Erie Yacht Club | Unclassified | $5.6M | $19.8M |
| 60 | Erie Rise Leadership Academy Charter School | Education | $5.6M | $3.7M |
| 61 | Dr Gertrude a Barber Foundation INC | Health Care | $5.4M | $80.8M |
| 62 | Sisters of St. Joseph Mission and Ministries Foundation | Unclassified | $5.3M | $18.2M |
| 63 | Hamot Health Foundation | Health Care | $5.0M | $26.4M |
| 64 | North East Community Foundation | Philanthropy, Voluntarism & Grantmaking | $4.8M | $17.2M |
| 65 | City Mission of the Evangelical Churches of Erie Pa | Unclassified | $4.7M | $9.3M |
| 66 | Ehca Foundation | Housing & Shelter | $4.5M | $12.5M |
| 67 | Lake Shore Country Club | Unclassified | $4.4M | $5.6M |
| 68 | Free to Choose Network | Arts, Culture & Humanities | $4.1M | $3.6M |
| 69 | Catholic Charities Counseling and Adoption Services INC | Unclassified | $4.1M | $1.1M |
| 70 | Mercy Center for Women INC | Unclassified | $4.0M | $10.1M |
| 71 | Erie Family Center | Human Services | $3.8M | $893K |
| 72 | Fulton Athletic Club | Recreation & Sports | $3.6M | $1.2M |
| 73 | Union City Community Foundation | Unclassified | $3.6M | $7.0M |
| 74 | Erie Philharmonic | Arts, Culture & Humanities | $3.6M | $4.5M |
| 75 | St. Martin Center | Human Services | $3.6M | $4.3M |
| 76 | Public Broadcasting of Northwest Pennsylvania INC | Arts, Culture & Humanities | $3.5M | $6.8M |
| 77 | Erie Downtown Development Corporation | Community Improvement & Capacity Building | $3.5M | $6.9M |
| 78 | Housing and Neighborhood Development Service | Housing & Shelter | $3.4M | $13.7M |
| 79 | Multicultural Community Resource Center | Unclassified | $3.3M | $3.1M |
| 80 | Northwestern Legal Services Fka Legal Services for North Western P | Crime & Legal-Related | $3.3M | $2.6M |
| 81 | Erster Siebenburger Gesang Verein | Recreation & Sports | $3.3M | $926K |
| 82 | Early Connections INC | Unclassified | $3.3M | $2.7M |
| 83 | Erie Area Convention and Visitors Bureau | Unclassified | $3.2M | $4.2M |
| 84 | Erie Independence House INC | Unclassified | $3.2M | $4.2M |
| 85 | Community Shelter Services INC | Housing & Shelter | $3.0M | $3.8M |
| 86 | Erie United Methodist Alliance | Religion-Related | $2.9M | $2.7M |
| 87 | Northwestern Pennsylvania Industrial Resource Center INC | Community Improvement & Capacity Building | $2.9M | $4.0M |
| 88 | Erie Day School INC | Education | $2.9M | $5.5M |
| 89 | Erie Club | Unclassified | $2.8M | $1.8M |
| 90 | Foundation for Free Enterprise Education | Youth Development | $2.6M | $7.9M |
| 91 | Northwestern Pennsylvania Humane Society | Animal-Related | $2.6M | $3.2M |
| 92 | Lawrence Park Golf Club | Unclassified | $2.5M | $2.3M |
| 93 | Merwin Foundation | Unclassified | $2.5M | $6.5M |
| 94 | Mary M Ogle Scholarship Fund Tr | Philanthropy, Voluntarism & Grantmaking | $2.4M | $3.7M |
| 95 | Arts Council of Erie | Arts, Culture & Humanities | $2.4M | $4.8M |
| 96 | Crime Victim Center of Erie County INC | Mental Health & Crisis Intervention | $2.4M | $5.7M |
| 97 | Childrens Advocacy Center of Erie County INC | Human Services | $2.3M | $2.6M |
| 98 | Anna Foundation INC | Animal-Related | $2.2M | $285K |
| 99 | Kuhl Hose Co | Public Safety, Disaster Preparedness & Relief | $2.2M | $1.9M |
| 100 | Cascade Park Club INC | Recreation & Sports | $2.1M | $323K |