| 1 | Council of International Schools INC | Education | $9.5M | $7.0M |
| 2 | Humane Society of Summit County | Animal-Related | $8.6M | $26.1M |
| 3 | Dalton Family Foundation | Philanthropy, Voluntarism & Grantmaking | $5.4M | $4.8M |
| 4 | City Mission Endowment Foundation | Human Services | $4.8M | $6.2M |
| 5 | Ambassadors Football | Recreation & Sports | $3.4M | $3.6M |
| 6 | Robert a Immerman Family Foundation | Philanthropy, Voluntarism & Grantmaking | $2.1M | $33.2M |
| 7 | Tinkers Creek Watershed Partnersinc | Unclassified | $1.6M | $395K |
| 8 | Roger K Williams Charitable Foundation | Arts, Culture & Humanities | $1.5M | $7.3M |
| 9 | Ohio Municipal Advisory Council | Community Improvement & Capacity Building | $909K | $2.7M |
| 10 | Operation Keepsake INC | Human Services | $725K | $444K |
| 11 | Our Community Hunger Center INC | Food, Agriculture & Nutrition | $452K | $108K |
| 12 | Twins Days INC | Recreation & Sports | $322K | $1.5M |
| 13 | Relink | Public & Societal Benefit | $240K | $85K |
| 14 | Gamma Kappa House Corporation of Theta Chi Fraternity | Education | $231K | $277K |
| 15 | Twinsburg Athletic Association of Twinsburg School District | Recreation & Sports | $225K | $52K |
| 16 | Twinsburg Baseball League INC | Recreation & Sports | $203K | $129K |
| 17 | Cleveland Community Access Corporation | Unclassified | $200K | $2.1M |
| 18 | Twinsburg Athletic Club Soccer Association INC | Recreation & Sports | $197K | $1K |
| 19 | Ohio Mortgage Bankers Association | Unclassified | $176K | $87K |
| 20 | International Football Foundation | Recreation & Sports | $158K | $62K |
| 21 | United Sikh Council of Greater Cleveland | Religion-Related | $150K | $0 |
| 22 | 221B Collaborations | Philanthropy, Voluntarism & Grantmaking | $146K | $272 |
| 23 | Twinsburg Chamber of Commerce | Unclassified | $123K | $104K |
| 24 | Twinsburg Community Improvement Corporation | Community Improvement & Capacity Building | $123K | $1.3M |
| 25 | The Twinsburg Vocal Music Boosters | Philanthropy, Voluntarism & Grantmaking | $113K | $53K |
| 26 | Twinsburg Band Boosters | Education | $111K | $131K |
| 27 | Twinsburg Public Library Foundation INC | Education | $100K | $342K |
| 28 | Developing Africa Together | International, Foreign Affairs & National Security | $98K | $109K |
| 29 | Jks Family Foundation | Philanthropy, Voluntarism & Grantmaking | $82K | $1.7M |
| 30 | Parent Booster USA INC | Unclassified | $81K | $8K |
| 31 | Twinsburg Recreational Basketballleague | Recreation & Sports | $76K | $28K |
| 32 | Twinsburg Schools Foundation INC | Education | $75K | $88K |
| 33 | Independence Community Theater | Arts, Culture & Humanities | $73K | $86K |
| 34 | PTA Ohio Congress | Unclassified | $72K | $35K |
| 35 | Hope Farm INC | Mental Health & Crisis Intervention | $61K | $60K |
| 36 | Beats for a Cure | Philanthropy, Voluntarism & Grantmaking | $61K | $16K |
| 37 | Children of Light | Education | $60K | $17K |
| 38 | Trinity Rose Foundation Incorporated | Medical Research | $54K | $8K |
| 39 | PTA Ohio Congress | Unclassified | $54K | $14K |
| 40 | Marspf Foundation | Philanthropy, Voluntarism & Grantmaking | $44K | $110K |
| 41 | PTA Ohio Congress | Education | $41K | $30K |
| 42 | John Patrick Carey Foundation | Philanthropy, Voluntarism & Grantmaking | $36K | $6K |
| 43 | Cesears Forum | Unclassified | $18K | $8K |
| 44 | Twinsburg Chamber of Commerce Foundation INC | Community Improvement & Capacity Building | $17K | $418 |
| 45 | John D Proctor Foundation | Unclassified | $14K | $277K |
| 46 | Rotary Club of Aurora Foundation | Philanthropy, Voluntarism & Grantmaking | $10K | $13K |
| 47 | Twinsburg Youth Wrestling INC | Recreation & Sports | $6K | $4K |
| 48 | Silent Mind Foundation | Recreation & Sports | $2K | $47K |
| 49 | Mark Kalina Jr Foundation | Human Services | $2K | $163K |
| 50 | Wellmore Charitable Foundation | Employment | $355 | $1 |
| 51 | International Entrepreneurial Investors Association | Arts, Culture & Humanities | $1 | $1 |
| 52 | Twinsburg Youth Softball League | Recreation & Sports | $0 | $0 |
| 53 | Haven of Hope Bible Church | Religion-Related | $0 | $0 |
| 54 | Destiny by Design Fellowship Center | Religion-Related | $0 | $0 |
| 55 | Twinsburg Education Association | Employment | $0 | $0 |
| 56 | Each One Reach One Mission | Religion-Related | $0 | $0 |
| 57 | Mosaic Neo | Religion-Related | $0 | $0 |
| 58 | Neo Church Plant | Religion-Related | $0 | $0 |
| 59 | Widows Sons Ohio Chapter II | Human Services | $0 | $0 |
| 60 | Free & Accepted Masons of Ohio | Unclassified | $0 | $0 |
| 61 | Royal Arch Masons of Ohio | Unclassified | $0 | $0 |
| 62 | Superior Treasures | Human Services | $0 | $0 |
| 63 | Templom Orzo INC | Religion-Related | $0 | $0 |
| 64 | Crimson Heights Ministries | Education | $0 | $0 |
| 65 | American Federation of State County & Municipal Employees | Unclassified | $0 | $0 |
| 66 | Buckeye House Rabbit Society | Animal-Related | $0 | $0 |
| 67 | Voltage Live | Arts, Culture & Humanities | $0 | $0 |
| 68 | Nfluenced Hearts Foundation | Human Services | $0 | $0 |
| 69 | Reset Transition Housing | Human Services | $0 | $0 |
| 70 | Cinematic Symphony Orchestra | Arts, Culture & Humanities | $0 | $0 |
| 71 | Forward Girls Leadership Academy | Education | $0 | $0 |
| 72 | Medical Insurance Industry Leading Equity in Science - Miiles | Education | $0 | $0 |
| 73 | Hands and Feet | Religion-Related | $0 | $0 |
| 74 | Falcon Flight Association | Youth Development | $0 | $0 |
| 75 | Twinsburg Charitable Fund | Community Improvement & Capacity Building | $0 | $0 |
| 76 | Connect Our Roots INC | Community Improvement & Capacity Building | $0 | $0 |
| 77 | Free & Accepted Masons of Ohio | Unclassified | $0 | $0 |
| 78 | Congregation Beth Aynu | Unclassified | $0 | $0 |
| 79 | Trinity Baptist Church | Unclassified | $0 | $0 |
| 80 | Garden Club of Ohio INC | Unclassified | $0 | $0 |
| 81 | The Mills Creek-east Association | Housing & Shelter | $0 | $0 |
| 82 | Substance of Life Enterprises INC C/o Betty L Jones | Education | $0 | $0 |
| 83 | Twinsburg Historical Society INC | Unclassified | $0 | $0 |
| 84 | Second Pilgrim Missionary Baptist Church INC | Unclassified | $0 | $0 |
| 85 | Fraternal Order of Eagles | Unclassified | $0 | $0 |
| 86 | Catholic Engaged Encounter of Cleveland INC | Unclassified | $0 | $0 |
| 87 | Mt Olive Missionary Baptist Church | Religion-Related | $0 | $0 |
| 88 | Top Ladies of Distinction INC | Unclassified | $0 | $0 |
| 89 | Friends of the Twinsburg Public Library | Education | $0 | $0 |
| 90 | International Association of Fire Fighters | Unclassified | $0 | $0 |
| 91 | Pneuma Life Foundation for the Prevention of Abusive Fam Envir | Human Services | $0 | $0 |
| 92 | Punjabi Cultural Society of Greater Cleveland INC | Arts, Culture & Humanities | $0 | $0 |
| 93 | Ss Comas & Damian Parish | Unclassified | $0 | $0 |
| 94 | Veterans of Foreign Wars of the United States Department of Ohio | Unclassified | $0 | $0 |
| 95 | Electrical Apparatus Service Association INC | Unclassified | $0 | $0 |
| 96 | Fraternal Order of Police Associates of Ohio Beachwood Lodge | Unclassified | $0 | $0 |
| 97 | Project Give | Human Services | $0 | $0 |
| 98 | Marion Motley Memorial Scholarship Foundation INC | Education | $0 | $0 |
| 99 | Press Play Orchestra | Arts, Culture & Humanities | $0 | $0 |
| 100 | PTA Ohio Congress | Unclassified | $0 | $0 |