| 1 | Myusa Credit Union INC | Unclassified | $18.5M | $329.6M |
| 2 | Middletown Community Foundation | Philanthropy, Voluntarism & Grantmaking | $8.8M | $44.1M |
| 3 | Marshall High School | Education | $8.6M | $10.8M |
| 4 | Woodside Cemetery Association | Unclassified | $7.6M | $25.1M |
| 5 | Fraternal Order of Eagles | Unclassified | $6.4M | $502K |
| 6 | Hospice Care of Middletown INC | Human Services | $5.9M | $6.1M |
| 7 | American Legion | Unclassified | $5.9M | $267K |
| 8 | Middletown Preparatory and Fitness Academy | Education | $5.7M | $87K |
| 9 | Middletown Lodge No 501 Loyal Order of Moose | Unclassified | $2.9M | $2.8M |
| 10 | Abilities First Foundation INC | Human Services | $2.8M | $1.5M |
| 11 | International Association of Machinists & Aerospace Workers | Unclassified | $2.6M | $7.0M |
| 12 | Wildwood Golf Club | Unclassified | $2.1M | $1.9M |
| 13 | Community Building Institute Middletown | Community Improvement & Capacity Building | $2.0M | $442K |
| 14 | Laborers International Union of North America | Unclassified | $1.7M | $3.4M |
| 15 | Lakota Robotics LLC | Unclassified | $1.6M | $1.8M |
| 16 | Hope House Rescue Mission INC | Human Services | $1.3M | $6.6M |
| 17 | International Association Registered Financial Consultants | Unclassified | $1.1M | $401K |
| 18 | River States Conference INC | Recreation & Sports | $1.0M | $146K |
| 19 | Fraternal Order of Eagles | Unclassified | $962K | $167K |
| 20 | 196 Middletown Area Schools Credit Union | Unclassified | $661K | $10.2M |
| 21 | Middletown Fine Arts Center | Arts, Culture & Humanities | $498K | $1.1M |
| 22 | Ohio Recreation and Education Foundation INC | Religion-Related | $461K | $2.4M |
| 23 | Safety Council of Southwestern Ohio | Community Improvement & Capacity Building | $456K | $343K |
| 24 | Compassionate Care Management INC | Human Services | $437K | $34K |
| 25 | Community Pregnancy Center | Health Care | $433K | $322K |
| 26 | Tim Sheets Ministries INC | Religion-Related | $378K | $1.1M |
| 27 | Feed the Hungry Project | Unclassified | $321K | $296K |
| 28 | Performing Arts Academy | Arts, Culture & Humanities | $320K | $1.6M |
| 29 | Albayan Academy | Education | $307K | $98K |
| 30 | Northstar Classical School INC | Education | $302K | $242K |
| 31 | Ohio Christian Academy INC | Education | $290K | $34K |
| 32 | Benevolent & Protective Order of Elks of the USA | Unclassified | $276K | $318K |
| 33 | Downtown Middletown INC | Community Improvement & Capacity Building | $272K | $113K |
| 34 | Freyas Hen House | Housing & Shelter | $232K | $189K |
| 35 | Middies Athletic Booster Club INC | Education | $223K | $108K |
| 36 | The Chamber Partnership for a Brighter Future | Unclassified | $216K | $256K |
| 37 | Junior Achievement of Middletown Area | International, Foreign Affairs & National Security | $213K | $203K |
| 38 | Parent Booster USA INC | Unclassified | $196K | $110K |
| 39 | Red Riding Hood Rescue Project | Animal-Related | $194K | $188K |
| 40 | Ohio Association for Court Administration INC | Unclassified | $185K | $246K |
| 41 | Ohio Valley Hockey Officials Assn INC | Education | $183K | $2K |
| 42 | Schwarber Family Foundation | Philanthropy, Voluntarism & Grantmaking | $173K | $160K |
| 43 | Cincy Galaxy | Recreation & Sports | $159K | $20K |
| 44 | Society of St. Vincent de Paul Holy Family Conference | Unclassified | $150K | $43K |
| 45 | Otto Storer Charitable Foundation INC | Health Care | $150K | $3.9M |
| 46 | Giving Without Boundaries | Human Services | $142K | $67 |
| 47 | Middletown Pickleball Association INC | Recreation & Sports | $139K | $148K |
| 48 | Fraternal Order of Police | Unclassified | $137K | $145K |
| 49 | Light Up Middletown INC | Recreation & Sports | $130K | $420K |
| 50 | International Association of Fire Fighters | Unclassified | $119K | $1 |
| 51 | Little League Baseball INC | Recreation & Sports | $118K | $120K |
| 52 | Fraternal Order of Police Associates 2 Middletown Ohio | Mutual & Membership Benefit | $114K | $52K |
| 53 | Butler County Bar Association | Social Science | $112K | $49K |
| 54 | Holiday Whopla INC | Community Improvement & Capacity Building | $105K | $63K |
| 55 | Free & Accepted Masons of Ohio | Unclassified | $98K | $966K |
| 56 | Atrium Auxiliary Middletown | Health Care | $91K | $35K |
| 57 | Butler County Warbirds INC | Arts, Culture & Humanities | $79K | $187K |
| 58 | Middletown Rising Stars Incorporated | Recreation & Sports | $72K | $44K |
| 59 | Currahee LLC | Human Services | $63K | $7K |
| 60 | Middletown Ohio Kiwanis Foundation | Unclassified | $62K | $44K |
| 61 | Inword Resources INC | Religion-Related | $62K | $28K |
| 62 | Compassion City Center INC | Human Services | $58K | $27K |
| 63 | Knights of Columbus | Unclassified | $58K | $26K |
| 64 | United Steelworkers | Unclassified | $57K | $111K |
| 65 | United Steelworkers | Unclassified | $51K | $43K |
| 66 | Art Central Foundation | Recreation & Sports | $50K | $86K |
| 67 | Family Services of Middletown | Human Services | $49K | $19K |
| 68 | Middletown Historical Society | Unclassified | $48K | $343K |
| 69 | Greater Cincinnati Mortgage Bankers Association | Community Improvement & Capacity Building | $47K | $41K |
| 70 | Integrity Empowerment Services | Religion-Related | $19K | $1 |
| 71 | Middletown Area Development Enterprise INC | Unclassified | $18K | $599K |
| 72 | Middletown Masonic Foundation | Youth Development | $10K | $8K |
| 73 | Breath of Life Mission | Religion-Related | $8K | $7K |
| 74 | Middletown Masonic Temple Co | Unclassified | $3K | $278K |
| 75 | Gaeke Foundation | Philanthropy, Voluntarism & Grantmaking | $350 | $2K |
| 76 | Lighthouse Ministries | Religion-Related | $0 | $0 |
| 77 | Kingdom Vision Ministries | Religion-Related | $0 | $0 |
| 78 | Links Theological University | Religion-Related | $0 | $0 |
| 79 | Mount Zion Missionary Baptist Church | Religion-Related | $0 | $0 |
| 80 | Kingswell Theological Seminary | Religion-Related | $0 | $0 |
| 81 | Sacred Dwelling Place | Religion-Related | $0 | $0 |
| 82 | Oaks Community Church | Religion-Related | $0 | $0 |
| 83 | First Church of God and Saints of Christ INC | Unclassified | $0 | $0 |
| 84 | Knights Templar of the United States of America | Unclassified | $0 | $0 |
| 85 | Madison Sports Club INC | Recreation & Sports | $0 | $0 |
| 86 | Community Chapel | Unclassified | $0 | $0 |
| 87 | Creekview Parent Teacher Organization | Education | $0 | $0 |
| 88 | Middletown First Church of the Nazarene | Unclassified | $0 | $0 |
| 89 | United Steelworkers | Unclassified | $0 | $0 |
| 90 | Order of the Eastern Star of Ohio & Jurisdiction Prince Hall Affiliati | Mutual & Membership Benefit | $0 | $0 |
| 91 | Order of the Eastern Star of Ohio | Unclassified | $0 | $0 |
| 92 | International Order of the Rainbow Girls | Unclassified | $0 | $0 |
| 93 | Sons of Italy in America | Unclassified | $0 | $0 |
| 94 | Fraternal Order of Eagles | Unclassified | $0 | $0 |
| 95 | Imperial Court 34 Order of the Amaranth | Mutual & Membership Benefit | $0 | $0 |
| 96 | Msbp INC | Community Improvement & Capacity Building | $0 | $0 |
| 97 | Middletown Aviation Foundation Corporation | Community Improvement & Capacity Building | $0 | $0 |
| 98 | 3 R Development Incorporated | Crime & Legal-Related | $0 | $0 |
| 99 | Murchinson Temple Christian Methodist Episcopal Church | Unclassified | $0 | $0 |
| 100 | Minimizing Animal Reproduction Without Extremes INC | Animal-Related | $0 | $0 |