| 1 | Central States Southeast & Southwest Areas Health & Welfare F | Unclassified | $56.5B | $16.6B |
| 2 | University of Chicago | Education | $10.5B | $15.0B |
| 3 | Northwestern Memorial Healthcare | Health Care | $10.1B | $14.2B |
| 4 | Ann & Robert H Lurie Childrens Hospital of Chicago | Health Care | $6.1B | $3.6B |
| 5 | Feeding America | Food, Agriculture & Nutrition | $5.2B | $573.7M |
| 6 | The Chicago Community Trust | Philanthropy, Voluntarism & Grantmaking | $4.0B | $6.0B |
| 7 | John D & Catherine T Macarthur Foundation | Philanthropy, Voluntarism & Grantmaking | $3.9B | $9.3B |
| 8 | Rush University Medical Center | Health Care | $3.9B | $4.7B |
| 9 | University of Chicago Medical Center | Health Care | $3.8B | $4.8B |
| 10 | Alliant Credit Union | Public & Societal Benefit | $3.0B | $19.5B |
| 11 | Builders Initiative | Philanthropy, Voluntarism & Grantmaking | $2.3B | $1.7B |
| 12 | Northwestern Memorial Healthcare | Health Care | $2.3B | $15.2B |
| 13 | Loyola University of Chicago | Education | $1.7B | $2.6B |
| 14 | American Medical Association | Unclassified | $1.7B | $1.5B |
| 15 | Depaul University | Education | $1.1B | $1.9B |
| 16 | Accenture United States Benefit | Mutual & Membership Benefit | $901.3M | $123.3M |
| 17 | Paul M Angell Family Foundation | Philanthropy, Voluntarism & Grantmaking | $867.0M | $903.3M |
| 18 | Chicago District Council of Carpenters Welfare Fund | Unclassified | $860.5M | $1.4B |
| 19 | National Association of Realtors | Unclassified | $840.0M | $1.1B |
| 20 | Art Institute of Chicago | Education | $828.0M | $2.1B |
| 21 | Misericordia Home | Human Services | $821.3M | $979.4M |
| 22 | Spencer Foundation | Education | $704.8M | $664.8M |
| 23 | Field Museum of Natural History | Arts, Culture & Humanities | $698.3M | $932.9M |
| 24 | Rehabilitation Institute of Chicago | Health Care | $656.6M | $1.3B |
| 25 | Illinois Institute of Technology | Education | $651.6M | $724.5M |
| 26 | Arie and Ida Crown Memorial | Unclassified | $622.0M | $1.4B |
| 27 | The Barack Obama Foundation | Arts, Culture & Humanities | $619.4M | $1.1B |
| 28 | Alzheimers Disease and Related Disorders Association INC | Diseases, Disorders & Medical Disciplines | $612.6M | $523.7M |
| 29 | Exelon Corporation Employees Benefit Trust for Union Employees | Unclassified | $568.1M | $709.2M |
| 30 | Kraft Foods Global INC Retiree Health and Life Benefit Tr | Mutual & Membership Benefit | $553.3M | $791.3M |
| 31 | United Airlines Employee Sick Leave Tr | Mutual & Membership Benefit | $539.7M | $61.6M |
| 32 | Mount Sinai Hospital Medical Center | Health Care | $428.3M | $318.2M |
| 33 | Joyce Foundation | Philanthropy, Voluntarism & Grantmaking | $419.7M | $1.3B |
| 34 | Pipe Fitters Welfare Fund Local 597 | Unclassified | $403.6M | $342.1M |
| 35 | National Opinion Research Center | Social Science | $398.4M | $225.0M |
| 36 | Elks National Foundation INC | Philanthropy, Voluntarism & Grantmaking | $377.2M | $885.0M |
| 37 | Chicago Symphony Orchestra | Arts, Culture & Humanities | $369.5M | $581.8M |
| 38 | International Fellowship of Christians & Jews | Unclassified | $354.0M | $233.0M |
| 39 | Exelon Corporation Employees Benefit Trust | Unclassified | $345.7M | $62.0M |
| 40 | Columbia College Chicago | Education | $342.4M | $476.6M |
| 41 | Greater Chicago Food Depository | Food, Agriculture & Nutrition | $338.7M | $216.0M |
| 42 | American Dental Association | Unclassified | $323.2M | $286.9M |
| 43 | Consumers Power Company Union Welfare Tr to Provide for Retiree | Unclassified | $318.2M | $868.4M |
| 44 | National Council of Young Mens Christian Assns of the USA | Human Services | $314.0M | $260.5M |
| 45 | Pert Foundation | Philanthropy, Voluntarism & Grantmaking | $313.3M | $317.3M |
| 46 | Robert R McCormick Foundation | Philanthropy, Voluntarism & Grantmaking | $302.5M | $1.8B |
| 47 | Hunter Family Foundation | Philanthropy, Voluntarism & Grantmaking | $299.7M | $420.5M |
| 48 | Kenneth C Griffin Museum of Science and Industry | Arts, Culture & Humanities | $285.4M | $433.0M |
| 49 | Terra Foundation for American Art | Philanthropy, Voluntarism & Grantmaking | $279.8M | $602.9M |
| 50 | Lurie Childrens Medical Group INC | Health Care | $267.5M | $27.0M |
| 51 | Noble Network of Charter Schools | Education | $266.9M | $268.6M |
| 52 | Moody Bible Institute of Chicago | Education | $249.0M | $549.9M |
| 53 | Pepco Holdings INC Veba Trust | Mutual & Membership Benefit | $247.0M | $331.7M |
| 54 | SEIU Local 1 & Participating Employers Health Trust | Unclassified | $246.0M | $171.7M |
| 55 | Lincoln Park Zoological Society | Animal-Related | $239.9M | $254.0M |
| 56 | American College of Surgeons | Education | $237.1M | $721.6M |
| 57 | National Council of State Boards of Nursing INC | Health Care | $236.4M | $325.7M |
| 58 | Thorek Memorial Hospital | Health Care | $233.2M | $618.7M |
| 59 | Caterpillar INC Group Insurance Master Trust Agreement | Unclassified | $230.8M | $87.8M |
| 60 | Jewish Federation of Metropolitan Chicago | Philanthropy, Voluntarism & Grantmaking | $228.4M | $1.1B |
| 61 | Ronald McDonald House Global | Health Care | $227.6M | $303.2M |
| 62 | Howard Brown Health Center | Unclassified | $224.4M | $178.5M |
| 63 | Shedd Aquarium Society | Animal-Related | $218.0M | $780.1M |
| 64 | Hyatt Corporation Welfare Benefit Trust | Unclassified | $217.3M | $16.4M |
| 65 | Pritzker Family Foundation | Philanthropy, Voluntarism & Grantmaking | $216.2M | $281.3M |
| 66 | Catholic Charities of the Archdiocese of Chicago | Unclassified | $215.7M | $214.7M |
| 67 | Community and Economic Development Association of Cook County INC | Community Improvement & Capacity Building | $215.2M | $60.4M |
| 68 | United Airlines Pilot Retiree Health Account Plan | Mutual & Membership Benefit | $211.6M | $953.0M |
| 69 | Kraft Foods North America INC Group Benefit Trust I | Unclassified | $205.5M | $14.9M |
| 70 | Polk Bros Foundation INC | Unclassified | $198.4M | $319.3M |
| 71 | Roosevelt University | Unclassified | $196.6M | $393.3M |
| 72 | SEIU Healthcare Il Home Care & Child Care Fund | Mutual & Membership Benefit | $180.8M | $277.2M |
| 73 | Saint Xavier University | Education | $175.8M | $164.4M |
| 74 | United Brotherhood of Carpenters & Joiners of America | Unclassified | $169.1M | $262.1M |
| 75 | Museum of Contemporary Art | Arts, Culture & Humanities | $169.1M | $210.4M |
| 76 | Jewish United Fund of Metropolitan Chicago | Philanthropy, Voluntarism & Grantmaking | $168.6M | $249.9M |
| 77 | La Rabida Childrens Hospital | Health Care | $166.8M | $278.7M |
| 78 | Saint Anthony Hospital | Health Care | $166.8M | $116.4M |
| 79 | Institute for the International Education of Students | Education | $166.6M | $140.8M |
| 80 | Access Community Health Network | Health Care | $165.6M | $81.3M |
| 81 | American Hospital Association INC | Unclassified | $164.9M | $435.3M |
| 82 | International Union of Operating Engineers Local 399 | Unclassified | $163.2M | $106.9M |
| 83 | Rush System for Health | Health Care | $162.9M | $41.5M |
| 84 | Pritzker Traubert Foundation | Philanthropy, Voluntarism & Grantmaking | $158.0M | $297.4M |
| 85 | Chicago Charter School Foundation | Education | $157.3M | $115.9M |
| 86 | Circle of Service Foundation | Unclassified | $156.1M | $592.0M |
| 87 | National Futures Association | Unclassified | $154.9M | $252.0M |
| 88 | Humboldt Park Health | Health Care | $152.2M | $149.1M |
| 89 | Coca-cola Co Postretireme Medical Voluntary Emp Beneficiary | Mutual & Membership Benefit | $145.6M | $41K |
| 90 | North Park University | Education | $145.6M | $331.1M |
| 91 | American Bar Association | Unclassified | $145.3M | $318.8M |
| 92 | American Lung Association | Diseases, Disorders & Medical Disciplines | $141.9M | $225.7M |
| 93 | Start Early | Unclassified | $141.3M | $129.4M |
| 94 | Lyric Opera of Chicago | Arts, Culture & Humanities | $139.8M | $266.3M |
| 95 | YMCA of Metropolitan Chicago | Human Services | $139.2M | $176.4M |
| 96 | Iff | Community Improvement & Capacity Building | $137.1M | $779.5M |
| 97 | Erie Family Health Center INC | Health Care | $132.6M | $126.0M |
| 98 | National Board of YMCA Employee Benefit Trust | Unclassified | $131.1M | $34.9M |
| 99 | Blue Cross Blue Shield Assoc Retire Benefit Trust | Unclassified | $130.7M | $98.4M |
| 100 | Planned Parenthood of Illinois | Health Care | $130.6M | $91.5M |