| 1 | Florida Health Sciences Center INC | Health Care | $5.0B | $5.5B |
| 2 | Shriners Hospitals for Children | Health Care | $3.6B | $11.9B |
| 3 | H Lee Moffitt Cancer Center and Research Institute Hospital INC | Health Care | $2.3B | $457.2M |
| 4 | St. Josephs Hospital INC | Health Care | $2.0B | $3.4B |
| 5 | University Community Hospital INC | Health Care | $1.4B | $1.3B |
| 6 | Suncoast Credit Union | Public & Societal Benefit | $1.4B | $17.8B |
| 7 | Baycare Medical Group INC | Health Care | $705.3M | $155.8M |
| 8 | Community Foundation of Tampa Bay INC | Philanthropy, Voluntarism & Grantmaking | $582.3M | $879.2M |
| 9 | Uma Education INC | Education | $577.5M | $743.2M |
| 10 | H Lee Moffitt Cancer Center and Research Institute INC | Diseases, Disorders & Medical Disciplines | $542.5M | $2.9B |
| 11 | University of Tampa | Education | $466.8M | $1.1B |
| 12 | University of South Florida Foundation INC | Education | $442.8M | $920.3M |
| 13 | Lutheran Services Florida INC | Human Services | $425.2M | $135.3M |
| 14 | University Medical Service Association INC | Education | $398.9M | $234.5M |
| 15 | Florida Hospital Physician Group INC | Unclassified | $328.3M | $59.4M |
| 16 | Central Florida Behavioral Health Network INC | Human Services | $298.6M | $41.0M |
| 17 | Feeding America Tampa Bay INC | Food, Agriculture & Nutrition | $213.5M | $70.9M |
| 18 | H Lee Moffitt Cancer Center and Research Institute Foundation INC | Diseases, Disorders & Medical Disciplines | $198.5M | $274.2M |
| 19 | Lifelink Foundation INC | Health Care | $197.8M | $291.6M |
| 20 | The Shriners Hospital for Children | Health Care | $169.9M | $566.6M |
| 21 | Special Operations Warrior Foundation | Education | $169.7M | $256.6M |
| 22 | Hillsborough County School Readiness Coalition INC | Education | $153.9M | $23.6M |
| 23 | H Lee Moffitt Cancer Ctr & Res Inst Life Time Cancer Scrn Ctr INC | Diseases, Disorders & Medical Disciplines | $145.7M | $16.6M |
| 24 | Arbitration Forums INC | Crime & Legal-Related | $121.4M | $104.4M |
| 25 | AAA Scholarship Foundation INC | Education | $108.4M | $157.3M |
| 26 | Tampa Family Health Centers INC | Health Care | $102.7M | $86.0M |
| 27 | Amikids INC | Unclassified | $89.1M | $68.4M |
| 28 | Drs Kiran & Pallavi Patel 2017 Foundation for Global Understandin | Education | $80.1M | $281.2M |
| 29 | Tampa General Medical Group INC | Health Care | $78.7M | $374.8M |
| 30 | St. Josephs Hospital of Tampa Foundation INC | Health Care | $77.4M | $73.8M |
| 31 | Tampa General Provider Network INC | Health Care | $77.1M | $39.0M |
| 32 | Amikids INC | Crime & Legal-Related | $69.7M | $51.1M |
| 33 | Tampa General Hospital Foundation INC | Health Care | $69.7M | $99.6M |
| 34 | Lions World Vision Institute INC | Unclassified | $67.4M | $54.1M |
| 35 | Big Brothers Big Sisters of America | Unclassified | $62.3M | $37.3M |
| 36 | Tampa Bay Performing Arts Center | Unclassified | $60.2M | $59.0M |
| 37 | Lowry Park Zoological Society of Tampa INC | Animal-Related | $57.7M | $83.5M |
| 38 | Ibis Healthcare INC | Mental Health & Crisis Intervention | $56.3M | $48.3M |
| 39 | Jaeb Center for Health Research INC | Unclassified | $54.2M | $21.1M |
| 40 | Tampa Metropolitan Area Young Mens Christian Association INC | Human Services | $53.6M | $79.7M |
| 41 | Chesapeake Bay Trust | Unclassified | $52.0M | $43.0M |
| 42 | Aacsb International the Association to Advance Collegiate Schools of | Education | $51.2M | $50.7M |
| 43 | Metropolitan Ministries INC | Human Services | $50.1M | $78.0M |
| 44 | Florida Aquarium INC | Animal-Related | $48.5M | $60.6M |
| 45 | Tampa Bay Performing Arts Center Foundation INC | Arts, Culture & Humanities | $47.8M | $76.2M |
| 46 | Vinik Family Foundation | Education | $42.6M | $183.3M |
| 47 | Senior Connection Center INC | Human Services | $42.4M | $11.8M |
| 48 | United Way Suncoast INC | Philanthropy, Voluntarism & Grantmaking | $36.2M | $52.8M |
| 49 | Floridians for Quality Virtual Education INC | Education | $35.6M | $14.5M |
| 50 | Agency for Community Treatment Services INC | Mental Health & Crisis Intervention | $33.9M | $27.1M |
| 51 | Tampa Museum of Art Foundation INC | Arts, Culture & Humanities | $33.8M | $63.6M |
| 52 | Bailey Family Foundation INC | Education | $33.3M | $63.8M |
| 53 | Interledger Foundation | International, Foreign Affairs & National Security | $32.9M | $97.5M |
| 54 | Tampa Bay Convention & Visitors Bureau INC | Unclassified | $32.5M | $12.0M |
| 55 | State Chartered Credit Unions in Florida | Unclassified | $31.8M | $600.6M |
| 56 | Tampa Preparatory School INC | Education | $31.6M | $73.6M |
| 57 | Carrollwood Day School Education INC | Education | $30.3M | $59.0M |
| 58 | St. Josephs Enterprises INC | Unclassified | $28.9M | $42.5M |
| 59 | St. Anthonys Professional Buildings & Services INC | Unclassified | $28.7M | $69.2M |
| 60 | St. Josephs Health Care Center INC | Health Care | $27.5M | $649.6M |
| 61 | Tampa-orlando-pinellas Jewish Foundation INC | Philanthropy, Voluntarism & Grantmaking | $26.4M | $86.7M |
| 62 | David a Straz Jr Foundation | Philanthropy, Voluntarism & Grantmaking | $25.7M | $131.7M |
| 63 | Usf Financing Corporation | Education | $25.6M | $558.4M |
| 64 | The Crisis Center of Tampa Bay INC | Mental Health & Crisis Intervention | $24.9M | $9.9M |
| 65 | Humane Society of Tampa Bay INC | Animal-Related | $24.5M | $30.6M |
| 66 | Tampa Museum of Art INC | Arts, Culture & Humanities | $23.7M | $37.5M |
| 67 | Taneja Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $23.6M | $44.1M |
| 68 | Childrens Home INC | Mental Health & Crisis Intervention | $23.5M | $13.9M |
| 69 | Jaeb Center Research Trust INC | Recreation & Sports | $22.5M | $35.8M |
| 70 | Cove Behavioral Health INC | Mental Health & Crisis Intervention | $22.0M | $23.6M |
| 71 | American Board of Pathology INC | Unclassified | $21.3M | $26.7M |
| 72 | Family First INC | Human Services | $21.3M | $15.7M |
| 73 | Palma Ceia Golf and Country Club | Unclassified | $20.4M | $32.0M |
| 74 | Concordia of Florida INC | Housing & Shelter | $20.1M | $26.0M |
| 75 | Bay Area Youth Services INC | Unclassified | $19.3M | $5.8M |
| 76 | Corbett Preparatory School of Ids INC | Education | $19.2M | $29.4M |
| 77 | Tampa Bay Workforce Alliance INC | Public & Societal Benefit | $19.1M | $5.0M |
| 78 | Girl Scouts of West Central Florida INC | Unclassified | $18.4M | $21.6M |
| 79 | The Building Industry Consulting Service International Incorporated | Public & Societal Benefit | $18.2M | $30.5M |
| 80 | Ronald & Joyce Wanek Foundation Ltd | Philanthropy, Voluntarism & Grantmaking | $17.8M | $92.8M |
| 81 | Boy Scouts of America | Youth Development | $17.7M | $35.1M |
| 82 | Hyer Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $17.6M | $18.6M |
| 83 | International Society for Pharmaceutical Engineering INC | Unclassified | $17.3M | $16.9M |
| 84 | Florida Reliability Coordinating Council | Unclassified | $17.3M | $11.0M |
| 85 | University of South Florida Research Foundation INC | Education | $17.2M | $187.8M |
| 86 | Bay Area Legal Services INC | Unclassified | $16.9M | $14.3M |
| 87 | Florida West Coast Public Broadcasting INC | Arts, Culture & Humanities | $16.5M | $54.3M |
| 88 | Usf Health Professions Conferencing Corporation | Education | $16.5M | $17.9M |
| 89 | Tampa Yacht & Country Club INC | Unclassified | $16.1M | $28.0M |
| 90 | Adventhealth Riverview INC | Unclassified | $15.9M | $339.2M |
| 91 | American Youth Academy INC | Education | $15.8M | $23.6M |
| 92 | Ronald McDonald House Charities of Tampa Bay INC | Health Care | $15.7M | $30.8M |
| 93 | Cambridge Christian School INC | Education | $15.6M | $24.3M |
| 94 | Florida Education Fund INC | Education | $15.1M | $34.9M |
| 95 | Healthy Start Coalition of Hillsborough County INC | Human Services | $15.0M | $7.7M |
| 96 | The Pepin Academies INC | Education | $14.8M | $27.5M |
| 97 | Florida Business Development Corporation | Community Improvement & Capacity Building | $14.6M | $38.3M |
| 98 | Macdonald Training Center INC | Human Services | $14.4M | $5.7M |
| 99 | Genspiration Foundation INC | Philanthropy, Voluntarism & Grantmaking | $14.2M | $29.0M |
| 100 | Northside Behavioral Health Center INC | Mental Health & Crisis Intervention | $14.0M | $4.7M |