| 1 | Orlando Health INC | Health Care | $5.3B | $9.5B |
| 2 | Orlando Health Medical Group INC | Health Care | $1.1B | $474.3M |
| 3 | Oneblood INC | Health Care | $551.2M | $491.6M |
| 4 | Assistance Fund INC | Health Care | $343.8M | $508.6M |
| 5 | Truist Foundation INC | Philanthropy, Voluntarism & Grantmaking | $290.9M | $547.9M |
| 6 | State Chartered Credit Unions in Florida | Unclassified | $273.6M | $4.7B |
| 7 | Goodwill Industries of Central Florida INC | Unclassified | $242.3M | $248.0M |
| 8 | Second Harvest Food Bank of Central Florida INC | Unclassified | $179.5M | $56.1M |
| 9 | Presbyterian Retirement Communities INC | Human Services | $177.4M | $573.7M |
| 10 | Orange County School Readiness Coalition | Education | $147.8M | $18.1M |
| 11 | Orlando Physicians Network INC | Unclassified | $142.8M | $35.2M |
| 12 | Orlando Health Foundation INC | Health Care | $130.3M | $278.6M |
| 13 | Central Florida Cares Health System INC | Human Services | $120.8M | $29.4M |
| 14 | Independent Colleges & Universities Benefits Association INC | Unclassified | $118.9M | $49.7M |
| 15 | Orlando Orange County Convention & Visitors Bureau INC | Unclassified | $113.7M | $51.5M |
| 16 | Aspire Health Partners INC | Mental Health & Crisis Intervention | $112.5M | $92.8M |
| 17 | University of Central Florida Foundation INC | Education | $106.8M | $659.7M |
| 18 | Ucf Athletic Association INC | Education | $106.7M | $88.8M |
| 19 | Community Coordinated Care for Children INC | Human Services | $93.7M | $9.3M |
| 20 | Childrens Home Society of Florida | Human Services | $91.6M | $76.6M |
| 21 | Florida Association of Realtors | Community Improvement & Capacity Building | $78.0M | $127.1M |
| 22 | Ana G Mendez University INC | Education | $75.9M | $44.1M |
| 23 | Lake Highland Preparatory School INC | Education | $73.6M | $138.5M |
| 24 | Dr Phillips Center for the Performing Arts INC | Arts, Culture & Humanities | $66.0M | $48.1M |
| 25 | Virginia Bankers Association Group Benefits Trust | Unclassified | $61.6M | $33.6M |
| 26 | Centeral Flordia Clinical Practice Organization INC | Education | $56.7M | $53.1M |
| 27 | Central Florida Young Mens Christian Association | Human Services | $56.0M | $97.7M |
| 28 | United Cerebral Palsy of Central Florida INC | Diseases, Disorders & Medical Disciplines | $51.5M | $79.6M |
| 29 | Adventhealth University INC | Unclassified | $49.3M | $45.5M |
| 30 | Westminster Retirement Communities Foundation INC | Housing & Shelter | $48.8M | $114.6M |
| 31 | Nonnas Garden a Private Foundation | Philanthropy, Voluntarism & Grantmaking | $48.8M | $72.8M |
| 32 | Arnies Army Charitable Events Foundation INC | Human Services | $43.7M | $24.0M |
| 33 | Discovery Education Services INC | Education | $40.7M | $154.8M |
| 34 | Central Florida Foundation INC | Philanthropy, Voluntarism & Grantmaking | $40.3M | $104.2M |
| 35 | Life Concepts INC | Human Services | $39.3M | $35.9M |
| 36 | Wesley Manor INC | Human Services | $38.5M | $88.7M |
| 37 | Dr Phillips INC | Philanthropy, Voluntarism & Grantmaking | $38.3M | $230.2M |
| 38 | Bliss Cares INC | Medical Research | $36.6M | $6.4M |
| 39 | Area Agency on Aging of Central Florida INC | Human Services | $35.7M | $9.2M |
| 40 | Orlando Lutheran Towers INC | Housing & Shelter | $35.7M | $82.6M |
| 41 | Central Florida Regional Workforce Development Board INC | Employment | $35.6M | $8.5M |
| 42 | The Catholic Foundation of Central Florida INC | Philanthropy, Voluntarism & Grantmaking | $34.6M | $140.5M |
| 43 | Ginsburg Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $34.4M | $13.4M |
| 44 | Suncoast Manor Retirement Community INC | Unclassified | $33.9M | $47.5M |
| 45 | Wm I H & Lula E Pitts Foundation | Unclassified | $33.8M | $52.8M |
| 46 | Unto INC | International, Foreign Affairs & National Security | $33.7M | $8.8M |
| 47 | Eastern States Veterinary Association INC | Education | $33.0M | $30.5M |
| 48 | International Association of Amusement Parks | Unclassified | $31.5M | $48.8M |
| 49 | Laura Berry Campbell Trust for Columbia Theological Seminary | Education | $30.9M | $78.6M |
| 50 | National Charitable Fund INC | Philanthropy, Voluntarism & Grantmaking | $30.6M | $17.9M |
| 51 | McKnight Brain Research Foundation | Philanthropy, Voluntarism & Grantmaking | $29.6M | $39.4M |
| 52 | Heritage Master Cemetery Perpetual Care Trust Tennessee | Mutual & Membership Benefit | $28.5M | $8.6M |
| 53 | University of Central Florida Research Foundation INC | Philanthropy, Voluntarism & Grantmaking | $27.8M | $38.4M |
| 54 | Westminster Shores INC | Human Services | $26.8M | $85.6M |
| 55 | Florida Citrus Sports Ventures INC | Community Improvement & Capacity Building | $26.2M | $10.1M |
| 56 | Camp-younts Fdnunrestricted Ua Tr | Unclassified | $26.0M | $44.8M |
| 57 | Homeless Services Network of Central Florida INC | Human Services | $25.7M | $7.3M |
| 58 | Boys & Girls Clubs of Central Florida Foundation INC | Youth Development | $25.6M | $68.0M |
| 59 | W I H Pitts Memorial Fund Ua 1125291 | Philanthropy, Voluntarism & Grantmaking | $25.5M | $78.4M |
| 60 | Valencia College Foundation | Unclassified | $25.4M | $117.6M |
| 61 | Oliver S & Jennie R Donaldson Charitable Trust | Unclassified | $24.3M | $35.8M |
| 62 | Westminster Pines INC | Housing & Shelter | $24.1M | $112.5M |
| 63 | Heart of Florida United Way | Philanthropy, Voluntarism & Grantmaking | $23.9M | $51.1M |
| 64 | Edward E Haddock Jr Family Foundation | Philanthropy, Voluntarism & Grantmaking | $23.3M | $46.2M |
| 65 | Edward E Hadddock Jr Family Operating Foundation | Environment | $23.3M | $38.3M |
| 66 | Country Club of Orlando | Unclassified | $22.6M | $34.3M |
| 67 | Jack Tarver Family Foundation | Human Services | $21.6M | $16.3M |
| 68 | Westminster Services INC | Housing & Shelter | $21.6M | $16.0M |
| 69 | George W Woodruff Tr Ua Fbo Walter F George Schl of Law Mercer University | Education | $20.9M | $42.2M |
| 70 | Westminster Senior Services INC | Housing & Shelter | $20.4M | $6.5M |
| 71 | Orlando Science Center INC | Arts, Culture & Humanities | $20.4M | $39.0M |
| 72 | Central Florida Kidney Centers INC | Diseases, Disorders & Medical Disciplines | $20.4M | $32.0M |
| 73 | Incharge Financial Solutions | Human Services | $20.2M | $8.5M |
| 74 | Boys & Girls Clubs of Central Florida | Youth Development | $19.7M | $38.7M |
| 75 | Orlando Regional Realtor Association INC | Unclassified | $19.5M | $63.9M |
| 76 | Palm Shores Retirement Community INC | Housing & Shelter | $19.4M | $53.2M |
| 77 | Community Legal Servies of Mid- Florida INC | Unclassified | $19.2M | $20.2M |
| 78 | Florida Community Loan Fund | Community Improvement & Capacity Building | $19.0M | $180.0M |
| 79 | Florida Citrus Sports Association INC | Unclassified | $18.8M | $28.7M |
| 80 | Laura Berry Campbell Trust Ua Fbo Berry College 04-15-25 | Philanthropy, Voluntarism & Grantmaking | $18.6M | $56.3M |
| 81 | Aggarwal & Gupta Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $18.2M | $65.8M |
| 82 | Crystal Lake Supportive Environments INC | Health Care | $18.2M | $7.3M |
| 83 | Florida Citrus Sports Events INC | Recreation & Sports | $18.1M | $29.8M |
| 84 | Health Care Center for the Homeless INC | Mental Health & Crisis Intervention | $17.8M | $14.8M |
| 85 | Princeton Homecare Services LLC | Unclassified | $17.5M | $10.5M |
| 86 | Leila a Thornton a Thornton Sr S M Venable W H Venable Memorial Fund | Unclassified | $17.3M | $23.7M |
| 87 | Katherine John Murphy Foundation Dtd 11-16-54 % Trust Co of Ga Ttee | Unclassified | $16.9M | $19.7M |
| 88 | J Bulou Campbell Tr Ua Dtd 9237 Fbo Rabun Gap Nacoochee School | Education | $16.9M | $17.0M |
| 89 | Seniors First INC | Human Services | $16.8M | $8.8M |
| 90 | Plough Foundation | Philanthropy, Voluntarism & Grantmaking | $16.8M | $21.2M |
| 91 | Coalition for the Homeless of Central Florida INC | Unclassified | $15.9M | $18.2M |
| 92 | Catholic Charities of Central Florida INC | Human Services | $15.3M | $26.0M |
| 93 | Christ for All Nations | Religion-Related | $15.0M | $8.1M |
| 94 | A Gift for Teaching INC | Education | $15.0M | $6.0M |
| 95 | Onic-hidden Cove INC | Housing & Shelter | $14.9M | $9.3M |
| 96 | Edna Wardlaw Charitable Tr Ua 123092 | Unclassified | $14.1M | $25.4M |
| 97 | Positive Assistance | Health Care | $14.1M | $3.7M |
| 98 | Health-isac INC | Community Improvement & Capacity Building | $13.7M | $9.5M |
| 99 | United Arts of Central Florida INC | Arts, Culture & Humanities | $13.6M | $13.8M |
| 100 | Lighthouse Works INC | Human Services | $13.5M | $3.7M |