| 1 | National Center for Construction Education Research | Employment | $32.9M | $39.0M |
| 2 | Regenerative Biologics INC | Health Care | $19.1M | $29.0M |
| 3 | Foundation for Applied Molecular Evolution INC | Education | $5.4M | $2.7M |
| 4 | Suwannee River Area Health Education Center INC | Health Care | $4.6M | $1.8M |
| 5 | Veterinary Hospital Managers Association INC | Unclassified | $2.4M | $2.9M |
| 6 | Alachua Learning Center INC | Education | $1.9M | $3.4M |
| 7 | Vaishnava Charitable Foundation INC | Religion-Related | $1.6M | $748K |
| 8 | National Ahec Organization INC | Health Care | $1.5M | $1.1M |
| 9 | Florida Alliance for Healthy Communities | Community Improvement & Capacity Building | $1.1M | $407K |
| 10 | Florida Farm Bureau Federation INC | Unclassified | $1.1M | $2.6M |
| 11 | Health Occupations Students of America | Unclassified | $1.0M | $1.0M |
| 12 | Retirement Home for Horses INC | Animal-Related | $1.0M | $13.3M |
| 13 | Bhaktivedanta Book Trust- International | Unclassified | $768K | $3.0M |
| 14 | Tylers Hope for Dystonia Cure INC | Diseases, Disorders & Medical Disciplines | $747K | $446K |
| 15 | Ultrasound School of North American Rheumatologists INC | Unclassified | $659K | $698K |
| 16 | Friends of Vrindavan INC | Religion-Related | $499K | $224K |
| 17 | Raymond E & Ellen F Crane Foundation | Unclassified | $436K | $3.6M |
| 18 | Robert L Oare Jr Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $377K | $1.7M |
| 19 | Perfect Seva INC | Human Services | $312K | $260K |
| 20 | Florida Association of Basketball Coaches INC | Recreation & Sports | $312K | $255K |
| 21 | Alachua Chamber of Commerce | Community Improvement & Capacity Building | $297K | $192K |
| 22 | Florida Farm Bureau Federation INC | Unclassified | $281K | $658K |
| 23 | Global Nerve Foundation | Diseases, Disorders & Medical Disciplines | $279K | $269K |
| 24 | Hutchinson Foundation INC | Housing & Shelter | $219K | $525K |
| 25 | One in Christ INC | Religion-Related | $180K | $686K |
| 26 | Lees Pre-school Center | Unclassified | $173K | $160K |
| 27 | Satvatove Institute INC | Education | $154K | $18K |
| 28 | Florida Public Health Association INC | Education | $154K | $148K |
| 29 | Supply and Multiply INC | International, Foreign Affairs & National Security | $152K | $22K |
| 30 | Inner Voice Productions INC | Religion-Related | $141K | $385K |
| 31 | Bbt Online Ministry INC | Religion-Related | $140K | $35K |
| 32 | Manos de Dios INC | Human Services | $121K | $11K |
| 33 | Florida Rural Health Association | Health Care | $121K | $48K |
| 34 | Back to Godhead | Religion-Related | $115K | $149K |
| 35 | Hitchcocks Community Fund INC | Philanthropy, Voluntarism & Grantmaking | $94K | $25K |
| 36 | New Technology Made Simple Now INC | Human Services | $93K | $28K |
| 37 | Parent Booster USA INC | Education | $84K | $32K |
| 38 | Sri Jagannath Chaitanya Sangha INC | Religion-Related | $81K | $104K |
| 39 | Gfwc Alachua Womans Club | Community Improvement & Capacity Building | $74K | $25K |
| 40 | The Association for the Protection of Children INC | Crime & Legal-Related | $73K | $41K |
| 41 | Vaishnava International Relief Association INC | Religion-Related | $72K | $20K |
| 42 | Alachua Raiders Unlimited Football and Cheer INC | Recreation & Sports | $71K | $3K |
| 43 | Pure Bhakti Archive | Religion-Related | $40K | $136K |
| 44 | Hitchcocks Charity Foundation | Philanthropy, Voluntarism & Grantmaking | $26K | $136K |
| 45 | Seva Care of Alachua INC | Human Services | $19K | $4K |
| 46 | Iskcon Youth Ministries | Religion-Related | $17K | $120K |
| 47 | Cultural Media Services INC | Unclassified | $10K | $6K |
| 48 | North Central Florida Advanced Practice Nurses INC | Community Improvement & Capacity Building | $0 | $0 |
| 49 | Radha Desh INC | Religion-Related | $0 | $0 |
| 50 | Free & Accepted Masons of Florida | Unclassified | $0 | $0 |
| 51 | Bhaktivedanta Gaudiya Institute INC | Religion-Related | $0 | $0 |
| 52 | Knights of Columbus | Unclassified | $0 | $0 |
| 53 | Canvas Church Fl INC | Religion-Related | $0 | $0 |
| 54 | Bhaktivedanta Academy of North America | Education | $0 | $0 |
| 55 | Teen Health and Nutrition | Youth Development | $0 | $0 |
| 56 | Alachua Lions Charitable Foundation INC | Health Care | $0 | $0 |
| 57 | Ayurveda Health Retreat INC | Health Care | $0 | $0 |
| 58 | PTA Florida Congress | Unclassified | $0 | $0 |
| 59 | Future Farmers of America State Associations & Local Chapter | Unclassified | $0 | $0 |
| 60 | Vision Ministries of Florida INC | Environment | $0 | $0 |
| 61 | Maxines Hideaway INC | Animal-Related | $0 | $0 |
| 62 | Raider Hoop Group INC | Recreation & Sports | $0 | $0 |
| 63 | Word of Deliverance Ministries | Religion-Related | $0 | $0 |
| 64 | International Association of Lions Clubs | Unclassified | $0 | $0 |
| 65 | Greaat INC | Health Care | $0 | $0 |
| 66 | Vietnam Veterans of America INC | Unclassified | $0 | $0 |
| 67 | Alliance of Divine Love INC | Unclassified | $0 | $0 |
| 68 | Birth Resource Network of Gainesville | Community Improvement & Capacity Building | $0 | $0 |
| 69 | Playmakers Athletics INC | Recreation & Sports | $0 | $0 |
| 70 | Shyam Radha Corp. | Community Improvement & Capacity Building | $0 | $0 |
| 71 | Myles Graham Foundation | Youth Development | $0 | $0 |
| 72 | Bhaktivedanta Gaudiya Institute of Alachua | Religion-Related | $0 | $0 |
| 73 | Alachua Womans Club Rose Petals INC | Human Services | $0 | $0 |
| 74 | Will Courtney Foundation | Education | $0 | $0 |
| 75 | Music & Arts Program for Youth | Youth Development | $0 | $0 |
| 76 | Bhaktivedanta Book Tr | Religion-Related | $0 | $0 |
| 77 | Cintamani Dham INC | Religion-Related | $0 | $0 |
| 78 | The Well of Life INC | Religion-Related | $0 | $0 |
| 79 | Christ Driven Purpose INC | Religion-Related | $0 | $0 |
| 80 | Bhaktiland INC | Human Services | $0 | $0 |
| 81 | Richard L Thompson Archives | Science & Technology | $0 | $0 |
| 82 | North Central Florida Model Railroad Club | Education | $0 | $0 |
| 83 | Temple of the Universe INC | Unclassified | $0 | $0 |
| 84 | Vaisnava Outreach INC | Religion-Related | $0 | $0 |
| 85 | Alachua Business League | Community Improvement & Capacity Building | $0 | $0 |
| 86 | International Society of Quantum Biology and Pharmacology | Unclassified | $0 | $0 |
| 87 | Forest Grove Baptist Church | Unclassified | $0 | $0 |
| 88 | Alachua First Baptist Church | Unclassified | $0 | $0 |
| 89 | New Oak Grove Baptist Church | Unclassified | $0 | $0 |
| 90 | Crusaders for Christ Church INC | Unclassified | $0 | $0 |
| 91 | Fr Paul V Donnelly Assembly No 1817 Fourth Degree Knights of Columbus | Mutual & Membership Benefit | $0 | $0 |
| 92 | River of Life Ag-trenton Campus | Unclassified | $0 | $0 |
| 93 | Pine Tree Industrial Park Property Owners Assn INC | Community Improvement & Capacity Building | $0 | $0 |
| 94 | Shaw Farms Homeowners Association | Housing & Shelter | $0 | $0 |
| 95 | Faith Hope & Charity Deliverance Center INC | Unclassified | $0 | $0 |
| 96 | Iskcon of Alachua County INC | Unclassified | $0 | $0 |
| 97 | Providence Center INC | Unclassified | $0 | $0 |
| 98 | Hague Community Cemetery Association INC | Unclassified | $0 | $0 |
| 99 | Blue Knights Motorcycle Club | Unclassified | $0 | $0 |
| 100 | Santa Fe Baptist Church | Unclassified | $0 | $0 |