| 1 | University of Wyoming Foundation Laramie | B | $1.6B | $113.6M | $980.2M |
| 2 | Newell B Sargent Foundation Worland | T | $440.8M | $0 | $15.7M |
| 3 | Ivinson Memorial Hospital Laramie | E | $217.5M | $161.6M | $417.9M |
| 4 | Powder River Energy Corporation Sundance | Z | $176.3M | $176.2M | $482.7M |
| 5 | Wyoming Educators Benefit Trust Cheyenne | Z | $149.3M | $139.2M | $49.5M |
| 6 | Natrona Collective Health Trust Casper | E | $143.2M | $0 | $288.3M |
| 7 | Community Foundation of Jackson Hole Jackson | T | $99.2M | $75.2M | $170.9M |
| 8 | Sargent Foundation for Episcopal Ministries Cheyenne | X | $90.6M | $1.6M | $8.3M |
| 9 | High West Energy INC Pine Bluffs | Z | $87.0M | $87.0M | $168.2M |
| 10 | High Plains Power INC Riverton | Z | $85.2M | $85.1M | $156.5M |
| 11 | Grand Teton National Park Foundation Moose | T | $85.2M | $24.5M | $26.3M |
| 12 | Powell Valley Health Care INC Powell | E | $82.7M | $82.7M | $34.4M |
| 13 | Lower Valley Energy INC Afton | Z | $80.2M | $80.2M | $318.7M |
| 14 | Pamela and Wayne Garrison Family Foundation Big Horn | T | $63.7M | $0 | $44.6M |
| 15 | Holdfast TR Jackson | C | $63.3M | $63.3M | $1.4B |
| 16 | Volunteers of America INC Sheridan | Z | $61.2M | $56.7M | $68.8M |
| 17 | Delta Dental Plan of Wyoming Cheyenne | Z | $57.6M | $51.8M | $26.3M |
| 18 | National Outdoor Leadership School Lander | C | $46.8M | $43.0M | $116.0M |
| 19 | Jackson Hole Classical School INC Jackson | B | $45.9M | $45.9M | $52.6M |
| 20 | Cumming Foundation Jackson | T | $41.7M | $0 | $243.3M |
| 21 | Hot Springs County Hospital Dist Thermopolis | E | $40.4M | $40.4M | $46.1M |
| 22 | Pharos Foundation Jackson | T | $38.1M | $0 | $31.6M |
| 23 | John P Ellbogen Foundation Casper | T | $31.0M | $0 | $61.2M |
| 24 | Cheyenne Frontier Days INC Cheyenne | Z | $28.1M | $26.8M | $46.8M |
| 25 | Wyrulec Company Torrington | Z | $26.2M | $26.2M | $57.0M |
| 26 | Cheyenne Leads Cheyenne | Z | $25.1M | $18.3M | $44.1M |
| 27 | Issa Foundation Cheyenne | P | $25.0M | $0 | $24.8M |
| 28 | Wyoming Community Foundation Laramie | T | $23.0M | $23.0M | $230.8M |
| 29 | Wyo Complex Casper | N | $22.9M | $22.5M | $43.9M |
| 30 | Whitney Benefits INC Sheridan | Z | $22.8M | $0 | $130.0M |
| 31 | Buffalo Bill Memorial Association Cody | A | $21.6M | $13.2M | $168.6M |
| 32 | McMurry Foundation Casper | T | $21.2M | $0 | $22.0M |
| 33 | Community Health Center of Central Wyoming INC Casper | E | $20.9M | $20.9M | $19.7M |
| 34 | The Lynn and Foster Friess Family Foundation Jackson | X | $20.5M | $0 | $125.6M |
| 35 | Wheatland Rural Electric Association Wheatland | Z | $20.4M | $20.4M | $43.2M |
| 36 | Hughes Charitable Foundation Jackson | T | $20.1M | $0 | $6.3M |
| 37 | Walking Softer Jackson | C | $19.8M | $0 | $34.5M |
| 38 | Teton Science Schools Jackson | C | $19.6M | $19.6M | $53.9M |
| 39 | National Museum of Wildlife Art of the United States Jackson | A | $19.5M | $6.7M | $43.7M |
| 40 | Cathedral Home for Children Laramie | P | $19.1M | $9.9M | $17.4M |
| 41 | Grand Teton Music Festival INC Jackson | A | $19.1M | $15.4M | $33.3M |
| 42 | Central Wyoming Counseling Center Casper | F | $17.7M | $13.9M | $18.5M |
| 43 | St. Johns Health Foundation Jackson | E | $17.6M | $12.1M | $24.7M |
| 44 | Carbon Power & Light INC Saratoga | Z | $17.6M | $17.6M | $60.1M |
| 45 | Big Horn Rural Electric Company Basin | Z | $17.4M | $17.4M | $44.9M |
| 46 | Niobrara Electric Association INC Lusk | W | $17.1M | $17.1M | $40.4M |
| 47 | Emerald Gate Charitable Trust Jackson | T | $16.8M | $0 | $178.9M |
| 48 | Wyoming Machinery Company Employee Medical Benefit Trust Casper | Z | $15.9M | $15.9M | $2.5M |
| 49 | Wyoming Child & Family Developement INC Guernsey | B | $15.4M | $15.4M | $10.9M |
| 50 | Wold Foundation Casper | Z | $14.8M | $0 | $53.1M |