| 1 | Shelby Cullom Davis Charitable Fund INC Wilmington | T | $36.6B | $0 | $3.1B |
| 2 | Astrazeneca Patient Assistance Organization Wilmington | E | $4.6B | $4.6B | $0 |
| 3 | Christiana Care Health Services INC Wilmington | E | $3.9B | $3.1B | $4.8B |
| 4 | University of Delaware Newark | Z | $3.0B | $0 | $4.6B |
| 5 | American Online Giving Foundation INC Newark | T | $2.1B | $2.1B | $269.5M |
| 6 | Bayhealth Medical Center INC Dover | E | $1.3B | $1.1B | $1.8B |
| 7 | Ge Li & Ning Zhao Family Foundation Wilmington | T | $844.2M | $0 | $309.6M |
| 8 | Beebe Medical Center INC Lewes | E | $644.7M | $634.9M | $723.8M |
| 9 | American Gift Fund Wilmington | T | $495.1M | $166.3M | $642.6M |
| 10 | Delaware State University Dover | B | $270.5M | $266.3M | $529.9M |
| 11 | Delaware Electric Cooperative INC Greenwood | Z | $254.9M | $254.8M | $452.2M |
| 12 | John & Tami Marick Foundation INC Wilmington | T | $254.6M | $0 | $408.4M |
| 13 | William T and Frances a Little Foundation Wilmington | T | $253.0M | $0 | $261.8M |
| 14 | Aurora Foundation Wilmington | T | $250.3M | $0 | $674.3M |
| 15 | The Champlin Foundation Wilmington | Z | $232.7M | $0 | $456.6M |
| 16 | Union Hospital of Cecil County Wilmington | E | $210.2M | $194.5M | $209.2M |
| 17 | Tidalhealth Nanticoke INC Seaford | E | $201.4M | $173.3M | $163.5M |
| 18 | Smilow Foundation INC Wilmington | T | $201.0M | $0 | $165.9M |
| 19 | Henry Francis du Pont Winterthur Museum INC Winterthur | A | $176.6M | $69.3M | $439.2M |
| 20 | St. Francis Hospital INC Wilmington | E | $161.8M | $161.5M | $75.6M |
| 21 | Wilmington University INC New Castle | B | $156.5M | $142.4M | $384.9M |
| 22 | Five Pointe Professional Liability Insurance Company Wilmington | E | $153.4M | $74.4M | $437.3M |
| 23 | The Grateful Lives Foundation Wilmington | T | $151.7M | $84.8M | $1.1B |
| 24 | Brandt Jackson Foundation Wilmington | T | $125.9M | $0 | $69.6M |
| 25 | Longwood Foundation INC Wilmington | T | $123.0M | $0 | $967.5M |
| 26 | Essex Avenue Foundation Wilmington | T | $108.3M | $0 | $616.1M |
| 27 | Unidel Foundation INC Greenville | B | $96.5M | $43.6M | $484.8M |
| 28 | Morgridge Family Foundation Wilmington | T | $93.0M | $0 | $243.9M |
| 29 | Head & Heart Foundation TR Wilmington | T | $91.9M | $0 | $245.4M |
| 30 | Band Foundation Wilmington | T | $86.6M | $0 | $147.8M |
| 31 | Delaware Community Foundation Wilmington | T | $79.8M | $32.2M | $346.1M |
| 32 | Daniel G and Wanda K Odell Family Foundation Wilmington | T | $78.5M | $0 | $10.4M |
| 33 | Moccasin Lake Foundation Wilmington | Z | $76.6M | $0 | $107.0M |
| 34 | Food for Education Foundation INC Dover | K | $74.9M | $74.9M | $93.5M |
| 35 | Raskob Foundation for Catholic Activities Incorporated Wilmington | Z | $74.8M | $0 | $208.0M |
| 36 | La Vida Feliz Foundation Wilmington | T | $72.3M | $0 | $376.6M |
| 37 | American Electric Power Foundation Wilmington | T | $71.3M | $0 | $65.2M |
| 38 | Institute of Cubergy Interactions Ici INC Wilmington | B | $70.2M | $0 | $20.2M |
| 39 | Ejk Foundation Wilmington | T | $65.7M | $0 | $51.9M |
| 40 | Welfare Foundation INC Wilmington | Z | $65.5M | $0 | $106.7M |
| 41 | Conexio Care INC Claymont | F | $64.4M | $63.9M | $44.6M |
| 42 | Pincus Family Fund Wilmington | T | $63.4M | $0 | $100.0M |
| 43 | Beebe Physican Network INC Lewes | E | $62.2M | $62.2M | $23.9M |
| 44 | Barry and Mimi Sternlicht Foundation Wilmington | T | $61.2M | $0 | $52.4M |
| 45 | Sunrise Project INC Wilmington | C | $61.1M | $61.1M | $14.0M |
| 46 | J B L Scholarship Trust Wilmington | Z | $60.9M | $0 | $10.4M |
| 47 | Lumena Foundation Wilmington | T | $60.1M | $0 | $30.3M |
| 48 | Zearn Claymont | B | $59.0M | $54.9M | $81.9M |
| 49 | Goodwill Industries of Delaware & Delaware County INC Wilmington | J | $58.0M | $57.9M | $53.2M |
| 50 | Jhc Foundation Wilmington | T | $57.7M | $0 | $114.0M |