| 51 | Bon Secours Mercy Health INC | Blue Ash, Ohio | $14.4B | $11.5B | $11.5B |
| 52 | William & Flora Hewlett Foundation | Menlo Park, California | $14.2B | $0 | $114.4M |
| 53 | Northwestern Memorial Healthcare | Chicago, Illinois | $14.2B | $10.1B | $10.1B |
| 54 | Adventist Health System Sunbelt INC | Altamonte Spg, Florida | $14.1B | $8.3B | $8.3B |
| 55 | Vanderbilt University | Nashville, Tennessee | $13.8B | $2.6B | $8.0B |
| 56 | Memorial Hermann Health System | Houston, Texas | $13.7B | $8.5B | $8.5B |
| 57 | Robert Wood Johnson Foundation | Princeton, New Jersey | $13.4B | $0 | $3.4B |
| 58 | J Paul Getty Trust | Los Angeles, California | $13.0B | $0 | $572.5M |
| 59 | Indiana University Health INC | Indianapolis, Indiana | $12.8B | $6.1B | $9.5B |
| 60 | Gs Donor Advised Philanthropy Fund for Wealth Management INC | New York, New York | $12.7B | $6.6B | $27.4B |
| 61 | Chancellor Masters and Scholars of the University of Cambridge | New York, New York | $12.6B | $3.1B | $3.1B |
| 62 | Adventist Health System Sunbelt Healthcare Corporation | Altamonte Spg, Florida | $12.5B | $3.2B | $3.2B |
| 63 | Cedars-sinai Medical Center | W Hollywood, California | $12.4B | $5.3B | $5.3B |
| 64 | Hudson East River Systems LLC | New York, New York | $12.4B | $156.9M | $2.6B |
| 65 | Silicon Valley Community Foundation | Mountain View, California | $12.3B | $4.9B | $19.0B |
| 66 | Trustees of the Estate of Bernice Pauahi Bishop | Honolulu, Hawaii | $12.2B | $588.8M | $4.7B |
| 67 | NYU Langone Hospitals | New York, New York | $12.1B | $9.5B | $14.4B |
| 68 | Stanford Health Care 227 | Stanford, California | $12.1B | $8.0B | $8.1B |
| 69 | Bloomberg Family Foundation INC | New York, New York | $12.1B | $0 | $1.2B |
| 70 | Trustees of Dartmouth College | Hanover, New Hampshire | $12.0B | $1.7B | $6.2B |
| 71 | Poudre Valley Health Care INC | Fort Collins, Colorado | $12.0B | $970.2M | $3.1B |
| 72 | Shriners Hospitals for Children | Tampa, Florida | $11.9B | $1.8B | $3.6B |
| 73 | The Methodist Hospital | Houston, Texas | $11.9B | $3.6B | $9.9B |
| 74 | St. Jude Childrens Research Hospital INC | Memphis, Tennessee | $11.7B | $2.0B | $2.0B |
| 75 | Gordon E and Betty I Moore Foundation | Palo Alto, California | $11.5B | $0 | $3.2B |
| 76 | Chancellor Masters & Scholars of the University of Oxford | Cary, North Carolina | $11.3B | $3.6B | $3.6B |
| 77 | Upmc | Pittsburgh, Pennsylvania | $11.2B | $24.3B | $25.7B |
| 78 | Foundation to Promote Open Society | New York, New York | $10.8B | $0 | $1.2B |
| 79 | William Marsh Rice University | Houston, Texas | $10.7B | $1.4B | $2.4B |
| 80 | University of Pittsburgh | Pittsburgh, Pennsylvania | $10.5B | $3.4B | $5.3B |
| 81 | Young Mens Christian Association Retirement Fund | New York, New York | $10.3B | $649.3M | $1.9B |
| 82 | Ohiohealth Corporation Grant Riverside Doctors Dublin Met | Columbus, Ohio | $10.2B | $5.1B | $7.9B |
| 83 | Sdg Impact Fund INC | Cartersville, Georgia | $10.1B | $13.6M | $13.6M |
| 84 | Childrens Hospital Medical Center | Cincinnati, Ohio | $10.0B | $3.4B | $4.6B |
| 85 | Brown University of Providence | Providence, Rhode Island | $10.0B | $1.6B | $4.6B |
| 86 | Upmc | Pittsburgh, Pennsylvania | $10.0B | $772.2M | $8.3B |
| 87 | American Lebanese Syrian Assoc Char INC | Memphis, Tennessee | $10.0B | $2.9B | $6.4B |
| 88 | Commonspirit Health | Englewood, Colorado | $9.9B | $3.4B | $3.4B |
| 89 | Texas Health Resources | Arlington, Texas | $9.9B | $1.6B | $4.1B |
| 90 | Mass General Brigham Incorporated | Somerville, Massachusetts | $9.8B | $1.9B | $1.9B |
| 91 | Rwj Barnabas Health INC | Oceanport, New Jersey | $9.7B | $7.8B | $7.8B |
| 92 | Childrens Healthcare of Atlanta INC | Brookhaven, Georgia | $9.7B | $183.7M | $3.7B |
| 93 | Sutter Health | Sacramento, California | $9.6B | $2.7B | $21.0B |
| 94 | Gavi Alliance | Washington, District of Columbia | $9.5B | $2.5B | $2.7B |
| 95 | Nature Conservancy | Arlington, Virginia | $9.5B | $1.5B | $4.0B |
| 96 | Orlando Health INC | Orlando, Florida | $9.5B | $4.8B | $5.3B |
| 97 | Providence Health & Services Washington | Renton, Washington | $9.3B | $9.7B | $9.8B |
| 98 | John D & Catherine T Macarthur Foundation | Chicago, Illinois | $9.3B | $0 | $3.9B |
| 99 | Jen-hsun & Lori Huang Foundation | Palo Alto, California | $9.2B | $0 | $6.0B |
| 100 | Memorial Sloan-kettering Cancer Center | New York, New York | $9.2B | $1.8B | $3.4B |