| 1 | The Methodist Hospital | Health Care | $9.9B | $11.9B |
| 2 | Memorial Hermann Health System | Health Care | $8.5B | $13.7B |
| 3 | Methodist Hospital Group | Unclassified | $4.4B | $7.6B |
| 4 | Texas Childrens Hospital | Health Care | $3.9B | $8.5B |
| 5 | Baylor College of Medicine | Education | $2.9B | $3.1B |
| 6 | William Marsh Rice University | Education | $2.4B | $10.7B |
| 7 | Community Health Choice Texas INC | Unclassified | $1.9B | $788.9M |
| 8 | Stoller Foundation | Philanthropy, Voluntarism & Grantmaking | $1.5B | $1.1B |
| 9 | Chi St. Lukes Health Baylor College of Medicine Medical Center | Health Care | $1.3B | $1.4B |
| 10 | Texas Gas Employee Welfare Benefits Trust | Unclassified | $1.2B | $87.1M |
| 11 | Methodist Hospital | Health Care | $1.0B | $1.0B |
| 12 | Schlumberger Master Health Care Trust | Unclassified | $954.4M | $728.4M |
| 13 | Community Health Choice INC | Unclassified | $898.4M | $280.6M |
| 14 | Texas Childrens Physician Group | Health Care | $780.7M | $117.6M |
| 15 | Bakerripley | Human Services | $643.0M | $186.5M |
| 16 | Laura and John Arnold Foundation | Philanthropy, Voluntarism & Grantmaking | $621.7M | $4.8B |
| 17 | Harmony Public Schools | Education | $599.4M | $1.2B |
| 18 | Greater Houston Community Foundation | Philanthropy, Voluntarism & Grantmaking | $567.5M | $1.1B |
| 19 | Sarofim Foundation | Philanthropy, Voluntarism & Grantmaking | $567.2M | $2.6B |
| 20 | Museum of Fine Arts Houston | Arts, Culture & Humanities | $500.5M | $2.3B |
| 21 | Kipp Texas INC | Education | $483.3M | $879.7M |
| 22 | Bp Corporation North America INC Veba Master Trust | Mutual & Membership Benefit | $459.8M | $26.3M |
| 23 | Houston Food Bank | Unclassified | $453.5M | $156.8M |
| 24 | Houston Endowment INC | Philanthropy, Voluntarism & Grantmaking | $427.4M | $2.5B |
| 25 | Legacy Community Health Services INC | Health Care | $370.8M | $148.3M |
| 26 | Texas Dow Employees Credit Union | Unclassified | $359.9M | $4.8B |
| 27 | St. Lukes Community Health Services | Health Care | $333.1M | $287.0M |
| 28 | Robert & Janice McNair Foundation | Unclassified | $326.9M | $330.6M |
| 29 | Memorial Hermann Medical Group | Health Care | $305.4M | $124.4M |
| 30 | Houston Livestock Show & Rodeo INC | Recreation & Sports | $301.7M | $492.2M |
| 31 | Yes Prep Public Schools INC | Education | $288.8M | $491.1M |
| 32 | Tch Pediatric Associates INC | Health Care | $261.3M | $96.3M |
| 33 | Brown Foundation INC | Philanthropy, Voluntarism & Grantmaking | $261.0M | $1.1B |
| 34 | Goodwill Industries of Houston | Employment | $224.9M | $107.3M |
| 35 | Young Mens Christian Association of Greater Houston Area | Human Services | $193.4M | $281.4M |
| 36 | Manned Space Flight Education Foundation Incorporated | Community Improvement & Capacity Building | $190.4M | $125.2M |
| 37 | U T Physicians | Health Care | $179.2M | $171.5M |
| 38 | Terry Foundation | Education | $168.7M | $433.1M |
| 39 | First Community Credit Union | Unclassified | $167.5M | $2.6B |
| 40 | Houston Christian University | Unclassified | $165.9M | $363.2M |
| 41 | Greater Houston Community Foundation | Unclassified | $153.9M | $231.1M |
| 42 | Methodist Primary Care Group | Philanthropy, Voluntarism & Grantmaking | $144.2M | $51.5M |
| 43 | Texas Medical Center | Health Care | $141.6M | $903.1M |
| 44 | University of St. Thomas | Education | $140.9M | $269.8M |
| 45 | Laurie and Reed Morian Foundation | Philanthropy, Voluntarism & Grantmaking | $140.4M | $152.4M |
| 46 | Gulf Coast Regional Blood Center | Health Care | $137.4M | $186.0M |
| 47 | South Texas College of Law Houston INC | Education | $135.9M | $118.0M |
| 48 | Investment & Research in Education INC | Religion-Related | $125.6M | $62.7M |
| 49 | Memorial Hermann Pharmacy Services LLC | Health Care | $125.2M | $22.5M |
| 50 | Texas Medical Center Central Heat and Cooling Services Corp. | Health Care | $119.0M | $549.7M |
| 51 | Kinder Foundation | Philanthropy, Voluntarism & Grantmaking | $118.5M | $173.4M |
| 52 | St. Johns School | Unclassified | $118.1M | $427.0M |
| 53 | John P McGovern Foundation | Unclassified | $117.2M | $138.4M |
| 54 | Lifegift Organ Donation Center | Health Care | $110.4M | $104.7M |
| 55 | Baylor St. Lukes Medical Group | Health Care | $109.2M | $50.3M |
| 56 | St. Lukes Hospital at the Vintage | Health Care | $107.6M | $58.7M |
| 57 | Memorial Hermann Hospital Based Physician Group | Health Care | $105.8M | $14.8M |
| 58 | Menil Foundation INC | Arts, Culture & Humanities | $105.2M | $507.0M |
| 59 | Fondren Foundation | Unclassified | $104.0M | $219.8M |
| 60 | Catholic Charities of the Archdiocese of Galveston-houston | Human Services | $103.0M | $60.5M |
| 61 | Hamill Foundation | Unclassified | $101.7M | $212.4M |
| 62 | Agreement of Trust for Maritime Association - Ila Welfare and Vaca | Employment | $101.3M | $47.4M |
| 63 | Houston Zoo INC | Animal-Related | $99.7M | $380.8M |
| 64 | John M Oquinn Foundation | Unclassified | $98.8M | $280.6M |
| 65 | Hildebrand Foundation | Philanthropy, Voluntarism & Grantmaking | $98.8M | $130.4M |
| 66 | Houston Ballet Foundation | Arts, Culture & Humanities | $94.7M | $181.5M |
| 67 | The Elkins Foundation | Unclassified | $93.2M | $206.6M |
| 68 | Robert a Welch Foundation 062491 | Philanthropy, Voluntarism & Grantmaking | $92.9M | $619.0M |
| 69 | First Service Credit Union | Unclassified | $91.1M | $1.6B |
| 70 | Memorial Hermann Foundation | Health Care | $90.5M | $196.5M |
| 71 | Cockrell Foundation | Unclassified | $89.0M | $176.7M |
| 72 | Episcopal Health Foundation | Health Care | $88.7M | $1.2B |
| 73 | United Way of Greater Houston | Philanthropy, Voluntarism & Grantmaking | $88.4M | $99.5M |
| 74 | The Awty International School | Unclassified | $88.1M | $235.9M |
| 75 | Houston Jewish Community Foundation | Philanthropy, Voluntarism & Grantmaking | $85.9M | $260.7M |
| 76 | George & Mary Josephine Hamman Foundation | Unclassified | $84.8M | $92.6M |
| 77 | John S Dunn Foundation | Unclassified | $82.1M | $81.8M |
| 78 | Dan L Duncan Family Foundation | Philanthropy, Voluntarism & Grantmaking | $81.0M | $700.5M |
| 79 | The Menninger Clinic Foundation | Health Care | $80.8M | $62.0M |
| 80 | Kinkaid School INC | Education | $80.4M | $243.0M |
| 81 | The Powell Foundation | Philanthropy, Voluntarism & Grantmaking | $77.8M | $451.5M |
| 82 | Houston Museum of Natural Science | Arts, Culture & Humanities | $75.6M | $299.4M |
| 83 | Zorich Family Foundation | Philanthropy, Voluntarism & Grantmaking | $70.8M | $109.3M |
| 84 | Texas Bay Credit Union | Unclassified | $67.2M | $852.8M |
| 85 | The Menninger Clinic | Health Care | $66.3M | $71.3M |
| 86 | Texas Heart Institute | Medical Research | $65.7M | $263.4M |
| 87 | Phillips Family Foundation | Philanthropy, Voluntarism & Grantmaking | $64.8M | $85.7M |
| 88 | Transport Workers Mta Health and Welfare Tr | Unclassified | $63.9M | $7.8M |
| 89 | William Stamps Farish Fund | Unclassified | $63.2M | $110.6M |
| 90 | Radoff Family Foundation | Philanthropy, Voluntarism & Grantmaking | $60.9M | $31.0M |
| 91 | Texas Pipe Trades Health Benefit Fund | Unclassified | $60.2M | $52.1M |
| 92 | Interfaith Ministries for Greater Houston | Unclassified | $60.1M | $27.8M |
| 93 | Dovetail Impact Foundation | Unclassified | $57.3M | $172.6M |
| 94 | Houston Parks Board | Philanthropy, Voluntarism & Grantmaking | $55.7M | $97.5M |
| 95 | The Lanier Theological Library Foundation | Philanthropy, Voluntarism & Grantmaking | $55.6M | $191.9M |
| 96 | University of Houston College of Business Foundation | Education | $55.3M | $109.7M |
| 97 | The 100 Club INC | Crime & Legal-Related | $53.8M | $61.9M |
| 98 | Cullen Trust for the Performing Arts | Arts, Culture & Humanities | $53.7M | $132.4M |
| 99 | Planned Parenthood Gulf Coast INC | Health Care | $53.0M | $55.8M |
| 100 | Centerpoint Energy Foundation INC | Philanthropy, Voluntarism & Grantmaking | $53.0M | $61.7M |