| 1 | University of Rochester | Education | $6.6B | $8.4B |
| 2 | Rochester General Hospital | Health Care | $1.9B | $2.0B |
| 3 | Rochester Institute of Technology | Education | $1.7B | $2.8B |
| 4 | The Unity Hospital of Rochester | Health Care | $738.1M | $469.7M |
| 5 | Highland Hospital of Rochester | Health Care | $547.3M | $573.7M |
| 6 | United Memorial Medical Center | Health Care | $199.2M | $160.5M |
| 7 | Heritage Christian Services INC | Human Services | $165.7M | $76.2M |
| 8 | St. John Fisher University | Education | $163.9M | $351.2M |
| 9 | Rochester Area Community Foundation | Philanthropy, Voluntarism & Grantmaking | $161.1M | $510.7M |
| 10 | Newark Wayne Community Hospital | Health Care | $157.8M | $144.8M |
| 11 | Hillside Childrens Center | Human Services | $143.1M | $116.6M |
| 12 | Clifton Springs Sanitarium Co | Health Care | $140.3M | $116.8M |
| 13 | Western New York Medical Practice Pc | Health Care | $132.2M | $27.5M |
| 14 | Nazareth University | Education | $130.2M | $291.8M |
| 15 | Catholic Charities of the Diocese of Rochester | Unclassified | $121.0M | $102.1M |
| 16 | Independent Living for Seniors INC | Religion-Related | $110.7M | $86.1M |
| 17 | Lifetime Assistance Incorporated | Human Services | $108.9M | $69.4M |
| 18 | Genesee Region Home Care Association INC | Health Care | $84.2M | $56.6M |
| 19 | Jewish Home of Rochester | Health Care | $83.0M | $116.2M |
| 20 | St. Anns Home for the Aged | Health Care | $77.3M | $115.2M |
| 21 | YMCA of Greater Rochester | Human Services | $72.5M | $142.7M |
| 22 | Foodlink INC | Food, Agriculture & Nutrition | $72.5M | $24.9M |
| 23 | Trillium Health INC | Unclassified | $70.8M | $53.4M |
| 24 | Roberts Wesleyan University | Education | $70.2M | $122.4M |
| 25 | United Association Employers Group Insurance Fund 13 | Unclassified | $69.9M | $67.2M |
| 26 | Goodwill of the Finger Lakes INC | Human Services | $63.9M | $49.9M |
| 27 | Rochester Regional Health Foundation | Health Care | $63.7M | $97.0M |
| 28 | Nysarc INC | Mental Health & Crisis Intervention | $62.5M | $38.2M |
| 29 | Greater Rochester Health Foundation | Philanthropy, Voluntarism & Grantmaking | $60.5M | $273.8M |
| 30 | United Way of Greater Rochester and the Finger Lakes INC | Philanthropy, Voluntarism & Grantmaking | $59.2M | $183.2M |
| 31 | Sidney Hillman Health Center of Rochester | Unclassified | $56.6M | $123.3M |
| 32 | Center for Disability Rights INC | Civil Rights, Social Action & Advocacy | $56.1M | $36.6M |
| 33 | Epilepsy-pralid INC | Human Services | $54.3M | $29.0M |
| 34 | Esl Charitable Foundation INC | Philanthropy, Voluntarism & Grantmaking | $50.2M | $106.8M |
| 35 | Jewish Senior Life Foundation INC | Philanthropy, Voluntarism & Grantmaking | $50.0M | $63.9M |
| 36 | The Rochester Friendly Home | Health Care | $49.2M | $46.0M |
| 37 | Rochester Regional Health | Health Care | $48.4M | $335.9M |
| 38 | Mary Cariola Childrens Center INC | Unclassified | $47.9M | $32.2M |
| 39 | Pathstone Corporation | Community Improvement & Capacity Building | $45.7M | $42.2M |
| 40 | Grhs Foundation INC | Community Improvement & Capacity Building | $44.5M | $139.5M |
| 41 | Coordinated Care Services INC | Unclassified | $41.2M | $16.8M |
| 42 | St. Johns Health Care Corporation | Health Care | $38.0M | $22.0M |
| 43 | Depaul Community Services INC | Mental Health & Crisis Intervention | $37.2M | $33.8M |
| 44 | Anthony L Jordan Health Corporation | Health Care | $36.2M | $25.4M |
| 45 | Mary M Gooley Hemophilia Center INC | Unclassified | $33.9M | $36.0M |
| 46 | Max and Marian Farash Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $31.2M | $341.9M |
| 47 | Planned Parenthood of Central and Western New York INC | Health Care | $29.3M | $28.4M |
| 48 | Episcopal Church Home | Health Care | $29.1M | $14.4M |
| 49 | Sands Family Supporting Foundation INC | Philanthropy, Voluntarism & Grantmaking | $28.3M | $110.2M |
| 50 | Oak Hill Country Club | Unclassified | $27.9M | $48.1M |
| 51 | Margaret Woodbury Strong Museum | Unclassified | $26.0M | $248.3M |
| 52 | Davenport-hatch Foundation INC | Unclassified | $25.7M | $39.5M |
| 53 | Electrical Workers Local No 86 Insurance Fund Plan and Trust | Unclassified | $24.5M | $32.2M |
| 54 | Starbridge Services INC | Unclassified | $23.8M | $9.4M |
| 55 | Mosaic Health INC | Health Care | $23.6M | $21.7M |
| 56 | Pathstone Corporation | Housing & Shelter | $23.5M | $150.7M |
| 57 | Park Ridge Nursing Home INC | Unclassified | $23.5M | $28.2M |
| 58 | Goodwill Vision Enterprises INC | Unclassified | $23.3M | $38.4M |
| 59 | Action for a Better Community INC | Human Services | $22.6M | $10.0M |
| 60 | Unity Ambulatory Surgery Center INC | Health Care | $22.4M | $16.4M |
| 61 | George Eastman Museum | Arts, Culture & Humanities | $22.1M | $64.3M |
| 62 | Genesee Valley Presbyterian Nursing Center | Health Care | $21.8M | $24.7M |
| 63 | Legal Assistance of Western New York INC | Crime & Legal-Related | $21.6M | $8.2M |
| 64 | Episcopal Church Home Foundation INC | Housing & Shelter | $21.3M | $22.0M |
| 65 | East House Corporation | Mental Health & Crisis Intervention | $20.9M | $29.7M |
| 66 | Jewish Community Center of Greater Rochester INC | Human Services | $20.7M | $32.8M |
| 67 | Wxxi Public Broadcasting Council | Arts, Culture & Humanities | $20.7M | $31.6M |
| 68 | Lifespan of Greater Rochester INC | Human Services | $19.8M | $14.9M |
| 69 | Eugenio Maria de Hostos Charter School | Education | $19.6M | $72.3M |
| 70 | Rochester Public Library | Education | $19.5M | $13.5M |
| 71 | Villa of Hope | Unclassified | $19.4M | $17.3M |
| 72 | Depaul Adult Care Communties INC | Health Care | $19.0M | $6.2M |
| 73 | United Cerebral Palsy Association of the Rochester Area INC | Diseases, Disorders & Medical Disciplines | $18.6M | $16.2M |
| 74 | Rochester School for the Deaf | Education | $18.6M | $19.0M |
| 75 | Center for Youth Services INC | Human Services | $18.6M | $11.9M |
| 76 | Rochester Police Benevolent Association Public Safety Building | Crime & Legal-Related | $18.5M | $27.3M |
| 77 | Monroe Community College Assn INC | Education | $18.2M | $46.4M |
| 78 | Ibero-american Action League INC | Human Services | $18.1M | $8.5M |
| 79 | Rochester Broadway Theatre League INC | Arts, Culture & Humanities | $18.0M | $20.7M |
| 80 | Meadows at Westfall INC | Health Care | $17.9M | $12.2M |
| 81 | The Harley School | Unclassified | $17.8M | $34.8M |
| 82 | Rochester Laborers Welfare-sub Fund | Unclassified | $17.8M | $23.2M |
| 83 | Rochester School of the Holy Childhood INC | Education | $16.7M | $41.1M |
| 84 | Jewish Community Federation of Greater Rochester Ny INC | Philanthropy, Voluntarism & Grantmaking | $16.7M | $53.2M |
| 85 | Finger Lakes Performing Provider System INC | Health Care | $16.6M | $17.3M |
| 86 | St. Anns Nursing Home Company INC | Unclassified | $16.3M | $48.6M |
| 87 | Habitat for Humanity International INC | Housing & Shelter | $15.5M | $14.8M |
| 88 | North Park Nursing Home INC | Health Care | $14.9M | $8.2M |
| 89 | Rochester Presbyterian Home INC | Human Services | $14.4M | $6.1M |
| 90 | Rochester Philharmonic Orchestra INC | Arts, Culture & Humanities | $14.2M | $25.2M |
| 91 | Golisano Business Coe INC | Education | $14.2M | $22.1M |
| 92 | Sheet Metal Workers Health Fund | Unclassified | $14.1M | $12.7M |
| 93 | Robert Owen Lehman Foundation INC | Philanthropy, Voluntarism & Grantmaking | $14.1M | $6.5M |
| 94 | Measures for Justice Institute | Civil Rights, Social Action & Advocacy | $14.0M | $12.7M |
| 95 | Rochester Academy Charter School | Education | $13.8M | $19.8M |
| 96 | Legal Aid Society of Rochester INC | Unclassified | $13.7M | $6.2M |
| 97 | Nextcorps INC | Social Science | $13.6M | $40.6M |
| 98 | Jewish Home of Rochester Senior Housing INC | Human Services | $13.4M | $24.8M |
| 99 | Wesley Gardens Corporation | Health Care | $13.3M | $6.7M |
| 100 | Empire Discovery Institute INC | Environment | $13.2M | $12.8M |