| 1 | Jackson Laboratory | Medical Research | $821.2M | $1.6B |
| 2 | Mount Desert Island Hospital | Health Care | $120.0M | $106.0M |
| 3 | Friends of Acadia | Environment | $34.0M | $97.3M |
| 4 | College of the Atlantic | Unclassified | $33.9M | $150.2M |
| 5 | Mount Desert Island Biological Laboratory | Science & Technology | $21.8M | $30.7M |
| 6 | Downeast Horizons INC | Mental Health & Crisis Intervention | $10.0M | $13.7M |
| 7 | Birch Bay Retirement Village | Unclassified | $6.4M | $5.8M |
| 8 | Mt Desert Island YMCA | Human Services | $2.4M | $5.7M |
| 9 | Abbe Museum | Arts, Culture & Humanities | $2.0M | $5.1M |
| 10 | Jesup Memorial Library | Education | $1.9M | $16.3M |
| 11 | YWCA of Mount Desert Island | Unclassified | $1.9M | $7.0M |
| 12 | Bar Harbor Food Pantry | Food, Agriculture & Nutrition | $1.1M | $2.3M |
| 13 | Bar Harbor Village Improvement Association | Community Improvement & Capacity Building | $948K | $1.3M |
| 14 | Bar Harbor Chamber of Commerce | Unclassified | $721K | $557K |
| 15 | Carl S Poole Jr Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $619K | $956K |
| 16 | Red Empress Foundation | Philanthropy, Voluntarism & Grantmaking | $535K | $1.3M |
| 17 | Kids Corner INC | Human Services | $535K | $67K |
| 18 | Bar Harbor Historical Society INC | Unclassified | $503K | $4.7M |
| 19 | The 1932 Criterion Theatre | Arts, Culture & Humanities | $474K | $2.8M |
| 20 | Island Connections | Human Services | $307K | $779K |
| 21 | Willowind Therapeutic Riding Center INC | Health Care | $304K | $792K |
| 22 | Acadia Community Association | Food, Agriculture & Nutrition | $270K | $484K |
| 23 | Open Table Mdi | Food, Agriculture & Nutrition | $220K | $77K |
| 24 | Ledgelawn Cemetery Incorporated | Unclassified | $202K | $2.1M |
| 25 | Artwaves | Arts, Culture & Humanities | $199K | $634K |
| 26 | Acadian Youth Sports | Youth Development | $162K | $113K |
| 27 | Knox District Housing | Unclassified | $133K | $349K |
| 28 | USA Swimming INC | Unclassified | $81K | $127K |
| 29 | Mid-coast Kennel Club of Maine Incorporated | Unclassified | $80K | $36K |
| 30 | Rotary International | Unclassified | $63K | $85K |
| 31 | Mount Desert Island Marathon INC | Philanthropy, Voluntarism & Grantmaking | $58K | $13K |
| 32 | Local Solutions | Unclassified | $53K | $248K |
| 33 | Ornithological Council | Animal-Related | $50K | $34K |
| 34 | Society for Human Ecology | Environment | $47K | $77K |
| 35 | Gaius Foundation INC | Philanthropy, Voluntarism & Grantmaking | $27K | $1.7M |
| 36 | Ten Sixty Six Charities | Philanthropy, Voluntarism & Grantmaking | $21K | $21K |
| 37 | Bar Harbor Yacht Club INC | Unclassified | $0 | $0 |
| 38 | St. Saviours Parish | Unclassified | $0 | $0 |
| 39 | Mount Desert Isle Chapter of the National Society of the Daughters | Arts, Culture & Humanities | $0 | $0 |
| 40 | First Baptist Church of Bar Harbor | Unclassified | $0 | $0 |
| 41 | Simpsom Cemetary Association | Unclassified | $0 | $0 |
| 42 | Mount Desert Island Search & Rescue | Public Safety, Disaster Preparedness & Relief | $0 | $0 |
| 43 | Hillside Cemetery Association INC | Mutual & Membership Benefit | $0 | $0 |
| 44 | Fraternal Order of Police | Public & Societal Benefit | $0 | $0 |
| 45 | First Light Assembly of God | Unclassified | $0 | $0 |
| 46 | Eden Baptist Church | Unclassified | $0 | $0 |
| 47 | American Legion Post 0025 George Edwin Kirk | Unclassified | $0 | $0 |
| 48 | American Legion Foundation Holding & Endowment Corp of Geo E Kirk-25 | Unclassified | $0 | $0 |
| 49 | Bar Harbor Garden Club | Unclassified | $0 | $0 |
| 50 | Cetos Research Organization | Science & Technology | $0 | $0 |
| 51 | Padmasambhava Buddhist Center of Maine | Unclassified | $0 | $0 |
| 52 | Ancient Free & Accepted Masons of Maine | Unclassified | $0 | $0 |
| 53 | Schools for Refugees | International, Foreign Affairs & National Security | $0 | $0 |
| 54 | Mdi Music Boosters | Education | $0 | $0 |
| 55 | Maine Documentary Film Collective | Arts, Culture & Humanities | $0 | $0 |
| 56 | Mdi Community Wood | Human Services | $0 | $0 |
| 57 | Bar Harbor Band | Arts, Culture & Humanities | $0 | $0 |
| 58 | Mdi Photo Club | Recreation & Sports | $0 | $0 |
| 59 | Bar Harbor Firefighters Association | Public Safety, Disaster Preparedness & Relief | $0 | $0 |
| 60 | American Association of University Women INC | Education | $0 | $0 |
| 61 | Bar Harbor Masonic Building Association | Unclassified | $0 | $0 |
| 62 | Bateau Press | Arts, Culture & Humanities | $0 | $0 |
| 63 | Downeast Food for Thought | Education | $0 | $0 |
| 64 | Professional Firefighters of Bar Harbor | Employment | $0 | $0 |
| 65 | Acadia Friends Monthly Meeting | Religion-Related | $0 | $0 |
| 66 | African Diaspora Scientists Federation INC | Science & Technology | $0 | $116K |
| 67 | God Chapter | Human Services | $0 | $0 |
| 68 | Nautilus Fund | Education | $0 | $0 |