| 1 | Johns Hopkins University | Education | $20.1B | $23.0B |
| 2 | The Johns Hopkins Hospital | Health Care | $3.7B | $3.6B |
| 3 | Sinai Hospital of Baltimore INC | Health Care | $1.2B | $811.2M |
| 4 | Johns Hopkins All Childrens Hospital INC | Health Care | $939.2M | $1.4B |
| 5 | Johns Hopkins Bayview Medical Center INC | Health Care | $807.9M | $459.3M |
| 6 | The Johns Hopkins Health System Corporation | Health Care | $714.8M | $2.2B |
| 7 | Jack and Nancy Dwyer Workforce Development Center INC | Health Care | $696.1M | $904.2M |
| 8 | Lucy Webb Haynes National Training School for Deaconesses and Mission | Health Care | $653.8M | $1.7B |
| 9 | Greater Baltimore Medical Center | Health Care | $638.4M | $901.3M |
| 10 | Mercy Medical Center INC | Health Care | $635.1M | $1.1B |
| 11 | Franklin Square Hospital Center INC | Health Care | $613.1M | $342.0M |
| 12 | T Rowe Price Program for Charitable Giving INC | Philanthropy, Voluntarism & Grantmaking | $587.1M | $648.3M |
| 13 | Johns Hopkins Medical Services Corporation | Health Care | $490.3M | $2.9M |
| 14 | Union Memorial Hospital | Health Care | $446.2M | $257.1M |
| 15 | Suburban Hospital Incorporated | Health Care | $398.6M | $694.3M |
| 16 | Loyola University Maryland INC | Unclassified | $393.2M | $751.8M |
| 17 | Johns Hopkins University | Unclassified | $350.9M | $216.1M |
| 18 | NFL Player Supplemental Disability Plan Trust | Unclassified | $349.5M | $92.3M |
| 19 | Howard County General Hospital INC | Health Care | $346.2M | $442.9M |
| 20 | Annie E Casey Foundation | Philanthropy, Voluntarism & Grantmaking | $336.0M | $2.3B |
| 21 | Sheppard Pratt Health System INC | Mental Health & Crisis Intervention | $334.8M | $430.5M |
| 22 | Johns Hopkins Community Physicians INC | Unclassified | $333.6M | $109.1M |
| 23 | Lutheran Immigration and Refugee Service | Unclassified | $304.3M | $111.9M |
| 24 | The Good Samaritan Hospital of Maryland INC | Health Care | $293.3M | $198.9M |
| 25 | Gene Upshaw NFL Player Health Reimbursement Account Plan Trust | Mutual & Membership Benefit | $265.8M | $1.1B |
| 26 | Kennedy Krieger Childrens Hospital INC | Health Care | $251.6M | $128.5M |
| 27 | Maryland Economic Development Corporation | Unclassified | $211.5M | $978.8M |
| 28 | Harbor Hospital INC | Health Care | $209.9M | $95.2M |
| 29 | World Relief Corporation O National | International, Foreign Affairs & National Security | $194.8M | $66.2M |
| 30 | Sheppard Pratt Investment INC | Community Improvement & Capacity Building | $177.9M | $159.7M |
| 31 | Chase Brexton Health Services INC | Diseases, Disorders & Medical Disciplines | $172.9M | $152.5M |
| 32 | Maryland Institute | Education | $170.0M | $321.3M |
| 33 | Meba Medical and Benefits Plan | Unclassified | $167.8M | $97.9M |
| 34 | Meba Vacation Plan Atlantic Gulf and Pacific Coasts | Employment | $163.4M | $61.9M |
| 35 | Goucher College | Education | $156.5M | $477.5M |
| 36 | Johns Hopkins University Retiree Medical Benefits Tr | Education | $145.3M | $324.2M |
| 37 | St. Paul Place Specialists INC | Unclassified | $144.8M | $48.9M |
| 38 | Pediatric Physicians Services INC | Unclassified | $143.2M | $7.5M |
| 39 | Johns Hopkins Pharmaquip INC | Health Care | $140.0M | $93.7M |
| 40 | The Abell Foundation INC | Unclassified | $134.3M | $296.4M |
| 41 | Stephen and Renee Bisciotti Foundation INC | Philanthropy, Voluntarism & Grantmaking | $123.9M | $474.4M |
| 42 | University of Maryland Faculty Physicians INC | Health Care | $120.7M | $374.6M |
| 43 | Chimes District of Columbia INC | Human Services | $115.9M | $74.9M |
| 44 | Maryland Food Bank INC | Unclassified | $112.8M | $69.0M |
| 45 | Young Mens Christian Association of Central Maryland INC | Human Services | $108.3M | $130.1M |
| 46 | Infinite Legacy INC | Health Care | $104.4M | $82.4M |
| 47 | Baltimore Regional Housing Partnership INC | Housing & Shelter | $103.9M | $30.2M |
| 48 | Keswick Foundation INC | Health Care | $100.2M | $248.0M |
| 49 | Levindale Hebrew Geriatric Ctr & Hospital INC | Health Care | $99.2M | $82.7M |
| 50 | Blind Industries & Services of Maryland | Human Services | $98.5M | $37.4M |
| 51 | Interchurch Medical Assistance INC | Human Services | $97.4M | $71.1M |
| 52 | NAACP Empowerment Programs INC | Civil Rights, Social Action & Advocacy | $95.1M | $89.5M |
| 53 | Baltimore Medical System INC | Unclassified | $93.5M | $45.2M |
| 54 | University of Maryland Medical System Foundation INC | Education | $93.1M | $104.0M |
| 55 | Mt Washington Pediatric Hospital INC | Health Care | $92.9M | $164.4M |
| 56 | Baltimore Community Foundation INC | Philanthropy, Voluntarism & Grantmaking | $90.1M | $314.3M |
| 57 | Health & Welfare Tr Fund for Intl Union of Operating Engineers | Unclassified | $87.6M | $89.9M |
| 58 | Mercy Health Services INC | Unclassified | $87.1M | $52.0M |
| 59 | Municipal Employees Credit Union of Baltimore INC | Unclassified | $82.2M | $1.3B |
| 60 | Notre Dame of Maryland University | Education | $79.1M | $160.2M |
| 61 | University of Maryland Baltimore Foundation INC | Education | $78.2M | $498.7M |
| 62 | France-merrick Foundation INC | Unclassified | $76.9M | $192.7M |
| 63 | The Harvey M Meyerhoff Fund INC | Philanthropy, Voluntarism & Grantmaking | $75.5M | $83.9M |
| 64 | Enoch Pratt Free Library of Baltimore City | Education | $75.2M | $205.8M |
| 65 | Maryland Emergency Medicine Network | Health Care | $74.8M | $8.5M |
| 66 | National Aquarium INC | Animal-Related | $74.4M | $101.5M |
| 67 | Maryland Legal Services Corp. | Crime & Legal-Related | $73.1M | $58.0M |
| 68 | Lutheran World Relief INC | International, Foreign Affairs & National Security | $72.1M | $124.2M |
| 69 | Maryland Family Care INC | Unclassified | $70.9M | $58.6M |
| 70 | Maryland School for the Blind | Education | $69.5M | $231.2M |
| 71 | Board of Child Care of the United Methodist Church Incorporated | Human Services | $69.0M | $114.9M |
| 72 | Kennedy Krieger Education and Community Services INC | Community Improvement & Capacity Building | $68.2M | $21.5M |
| 73 | Total Health Care INC | Health Care | $66.5M | $51.2M |
| 74 | University of Maryland Physicians Pa | Unclassified | $64.9M | $22.8M |
| 75 | Trustees of the Endowment Fund of the Univ of Md School of Medicine | Unclassified | $63.7M | $143.6M |
| 76 | Lieber Institute INC | Medical Research | $58.7M | $35.1M |
| 77 | Digital Harbor Foundation INC | Education | $57.9M | $57.3M |
| 78 | Associated Jewish Charities of Baltimore | Philanthropy, Voluntarism & Grantmaking | $57.2M | $422.3M |
| 79 | Friends School of Baltimore INC | Education | $56.0M | $109.0M |
| 80 | Gilman School INC | Education | $55.8M | $283.2M |
| 81 | The United Way of Central Maryland INC | Philanthropy, Voluntarism & Grantmaking | $55.0M | $54.6M |
| 82 | The Arc Baltimore INC | Human Services | $54.7M | $39.2M |
| 83 | Behavioral Health System Baltimore INC | Mental Health & Crisis Intervention | $54.3M | $31.6M |
| 84 | Associated Jewish Community Federation of Baltimore INC | Philanthropy, Voluntarism & Grantmaking | $54.1M | $45.8M |
| 85 | The Corporation for the Relief of the Widows & Children of the Clerg | Religion-Related | $53.9M | $44.3M |
| 86 | Chimes INC | Human Services | $52.4M | $26.0M |
| 87 | Bryn Mawr School for Girls of Baltimore City | Education | $51.3M | $113.7M |
| 88 | Roland Park Place INC | Human Services | $50.5M | $143.9M |
| 89 | Hugo W Moser Research Institute at Kennedy Krieger INC | Science & Technology | $50.0M | $10.0M |
| 90 | Richman Foundation INC | Philanthropy, Voluntarism & Grantmaking | $49.7M | $29.0M |
| 91 | Johns Hopkins Home Care Group INC | Unclassified | $48.3M | $124.1M |
| 92 | Park School of Baltimore INC | Education | $47.6M | $116.7M |
| 93 | Calvert School INC | Education | $47.3M | $134.5M |
| 94 | Legal Aid Bureau INC | Crime & Legal-Related | $46.4M | $37.8M |
| 95 | Marion I & Henry J Knott Foundation INC | Unclassified | $45.6M | $85.3M |
| 96 | Madison Street Properties INC | Community Improvement & Capacity Building | $45.0M | $194.7M |
| 97 | University of Maryland Surgical Associates P a | Health Care | $44.7M | $15.2M |
| 98 | Boys Latin School of Maryland Incorporated | Education | $43.3M | $139.6M |
| 99 | West Coast Neonatology INC | Unclassified | $43.2M | $20.9M |
| 100 | Baltimore Symphony Orchestra INC | Arts, Culture & Humanities | $43.1M | $22.2M |