| 1 | Owensboro Health Regional Hospital | Health Care | $1.0B | $2.8B |
| 2 | Owensboro Health Medical Group INC | Health Care | $126.8M | $406.3M |
| 3 | Owensboro Health Twin Lakes Regional Medical Center INC | Health Care | $72.1M | $145.8M |
| 4 | Audubon Area Community Services INC | Human Services | $59.4M | $64.4M |
| 5 | Rivervalley Behavioral Health INC | Mental Health & Crisis Intervention | $51.8M | $67.9M |
| 6 | Kentucky Wesleyan College | Education | $47.0M | $66.2M |
| 7 | Wendell Fosters Campus for Developmental Disabilities INC | Diseases, Disorders & Medical Disciplines | $27.7M | $24.5M |
| 8 | Brescia University INC | Education | $26.3M | $40.9M |
| 9 | Behavioral Healthcare Services INC | Human Services | $13.4M | $4.8M |
| 10 | Acumen Counseling Services INC | Mental Health & Crisis Intervention | $11.6M | $1.0M |
| 11 | Asmark Safety Organization INC | Food, Agriculture & Nutrition | $11.4M | $6.5M |
| 12 | Hospice & Palliative Care of the Ohio Valley INC | Human Services | $10.2M | $15.8M |
| 13 | Rivervalley Consulting Services INC | Public & Societal Benefit | $8.3M | $1.4M |
| 14 | Carmelite Sisters Ofthe Divine Heart of Jesus of Kentucky Corpora | Unclassified | $8.1M | $7.5M |
| 15 | Amvets | Unclassified | $6.1M | $417K |
| 16 | Puzzle Pieces INC | Human Services | $5.8M | $4.9M |
| 17 | Audubon Area Community Care Clinic INC | Health Care | $4.6M | $3.2M |
| 18 | Public Life Foundation of Owensboro INC | Philanthropy, Voluntarism & Grantmaking | $4.4M | $5.6M |
| 19 | Lawrence & Augusta Hager Educational Foundation INC | Unclassified | $4.0M | $6.4M |
| 20 | Society of St. Vincent de Paul Diocesan Council of Owensboro INC | Community Improvement & Capacity Building | $3.9M | $2.1M |
| 21 | International Bluegrass Music Museum INC | Arts, Culture & Humanities | $3.5M | $9.2M |
| 22 | Western Kentucky Regional Blood Center INC | Health Care | $3.5M | $3.2M |
| 23 | Investing in Communities | Housing & Shelter | $3.2M | $9.5M |
| 24 | Marilyn and William Young Charitable Foundation INC | Unclassified | $3.1M | $10.9M |
| 25 | Owensboro Area Shelter Information and Services INC Oasis | Unclassified | $3.0M | $3.4M |
| 26 | Owensboro Country Club | Unclassified | $3.0M | $6.1M |
| 27 | Veterans of Foreign Wars Dept of Kentucky | Unclassified | $2.8M | $2.5M |
| 28 | Riverpark Center INC | Arts, Culture & Humanities | $2.7M | $9.2M |
| 29 | Owensboro Health Foundation INC | Health Care | $2.5M | $7.4M |
| 30 | Cliff Hagan Boys & Girls Club INC | Youth Development | $2.3M | $10.0M |
| 31 | Michael E Horn Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $2.2M | $10.2M |
| 32 | Daniel Pitino Shelter INC | Housing & Shelter | $2.1M | $994K |
| 33 | St. Joseph Peace Mission for Children | Human Services | $1.9M | $1.5M |
| 34 | Family Y of Owensboro Daviess County INC | Unclassified | $1.7M | $5.5M |
| 35 | Green River Regional Rape Victim Services INC | Mental Health & Crisis Intervention | $1.7M | $1.5M |
| 36 | Owensboro Area Museum of Science and History INC | Arts, Culture & Humanities | $1.6M | $2.2M |
| 37 | United Way of the Ohio Valley INC | Philanthropy, Voluntarism & Grantmaking | $1.5M | $2.0M |
| 38 | Opportunity Center of Owensboro INC | Human Services | $1.4M | $2.5M |
| 39 | Margaret Kinchloe Holbrook Charitable Tr | Unclassified | $1.4M | $1.1M |
| 40 | Habitat for Humanity International INC | Housing & Shelter | $1.4M | $5.4M |
| 41 | Owensboro Museum of Fine Art Foundation INC | Philanthropy, Voluntarism & Grantmaking | $1.4M | $8.9M |
| 42 | Goodfellows Club of Owensboro Kentucky INC | Human Services | $1.4M | $1.5M |
| 43 | John B and Brownie Young Memorial Fund | Unclassified | $1.3M | $13.7M |
| 44 | Covenant Baptist Theological Seminary | Education | $1.3M | $742K |
| 45 | H L Neblett Community Center INC | Unclassified | $1.2M | $3.3M |
| 46 | Greater Owensboro Leadership Institute INC | Public & Societal Benefit | $1.2M | $706K |
| 47 | Civitan International | Philanthropy, Voluntarism & Grantmaking | $1.1M | $188K |
| 48 | Owensboro-daviess County Chamber of Commerce INC | Unclassified | $1.1M | $1.1M |
| 49 | Cigar Factory Complex INC | Unclassified | $1.0M | $9.3M |
| 50 | Western Kentucky Botanical Garden | Environment | $1.0M | $3.1M |
| 51 | International Brotherhood of Electrical Workers | Unclassified | $957K | $986K |
| 52 | Lester E Yeager Charitable Trust B | Unclassified | $905K | $4.9M |
| 53 | Windridge Country Club INC | Unclassified | $899K | $223K |
| 54 | Emerge Global | Community Improvement & Capacity Building | $880K | $214K |
| 55 | Delmas Clardy Memorial Unitrust Fbo Ky Wesleyan | Education | $860K | $2.0M |
| 56 | Owensboro Symphony Orchestra INC | Arts, Culture & Humanities | $849K | $2.4M |
| 57 | Boulware Mission INC | Human Services | $843K | $3.4M |
| 58 | Free & Accepted Masons of Kentucky | Unclassified | $843K | $834K |
| 59 | Girls Incorporated of Owensboro Daviess County | Education | $834K | $1.7M |
| 60 | United Association of Journeymen & Apprentices of the Plumbing & Pipe | Unclassified | $800K | $2.1M |
| 61 | Foundation for Excellence Owensboro Independent Schools | Education | $751K | $1.1M |
| 62 | Owensboro Community and Technical College Foundation INC | Education | $731K | $8.7M |
| 63 | Grace Christian Academy of Owensboro | Education | $721K | $189K |
| 64 | Senior Community Center of Owensboro-daviess County INC | Human Services | $708K | $450K |
| 65 | The Owensboro-daviess County Tennis Association INC | Recreation & Sports | $648K | $612K |
| 66 | Southeastern Allergy Asthma & Immunology Society | Diseases, Disorders & Medical Disciplines | $646K | $1.3M |
| 67 | Friends of Sinners INC | Mental Health & Crisis Intervention | $628K | $0 |
| 68 | Pregnancy Center of Owensboro | Health Care | $626K | $1.3M |
| 69 | Owensboro Museum of Fine Art INC | Arts, Culture & Humanities | $607K | $8.7M |
| 70 | Greater Owensboro Senior Citizens Union Labor Housing Corporation | Housing & Shelter | $565K | $16.2M |
| 71 | Laborers International Union of North America | Unclassified | $560K | $810K |
| 72 | Greater Owensboro Economic Development Corp. | Unclassified | $528K | $327K |
| 73 | Greater Owensboro Realtor Association INC | Unclassified | $496K | $662K |
| 74 | Center of Owensboro Daviess County | Human Services | $493K | $146K |
| 75 | Awaken Ministries INC | Religion-Related | $487K | $107K |
| 76 | Responsibleag INC | Food, Agriculture & Nutrition | $465K | $1.2M |
| 77 | Help Office of Owensboro INC | Unclassified | $428K | $980K |
| 78 | Kentucky Farm Bureau Federation | Unclassified | $426K | $1.3M |
| 79 | Impact 100-OWENSBORO INC | Philanthropy, Voluntarism & Grantmaking | $399K | $998K |
| 80 | Daviess County Public Library Foundation INC | Unclassified | $396K | $1.3M |
| 81 | Owensboro Dance Theater INC | Arts, Culture & Humanities | $389K | $339K |
| 82 | Theatre Workshop of Owensboro INC | Unclassified | $384K | $762K |
| 83 | Casa of Ohio Valley INC | Civil Rights, Social Action & Advocacy | $374K | $980K |
| 84 | Home Builders Association of Owensboro I INC | Unclassified | $369K | $218K |
| 85 | Fraternal Order of Police | Unclassified | $356K | $330K |
| 86 | Junior Achievement of West Kentucky INC | International, Foreign Affairs & National Security | $353K | $1.1M |
| 87 | Bakery Confectionery Tobacco | Unclassified | $344K | $240K |
| 88 | Issac Sterett Adventure Foundation Company | Human Services | $340K | $7.1M |
| 89 | Independence Foundation Incorporated | Philanthropy, Voluntarism & Grantmaking | $332K | $394K |
| 90 | Larry & Frankie Hager Trust 1 | Philanthropy, Voluntarism & Grantmaking | $331K | $649K |
| 91 | Green River Independent Living III INC | Human Services | $311K | $1.4M |
| 92 | Green River Area Beef Improvement Group INC | Animal-Related | $310K | $37K |
| 93 | Owensboro United Soccer Club INC | Unclassified | $304K | $195K |
| 94 | Mentorkids Kentucky INC | Youth Development | $295K | $709K |
| 95 | Larry & Frankie Hager Trust 2 | Philanthropy, Voluntarism & Grantmaking | $270K | $577K |
| 96 | Empowerment Academy INC | Human Services | $262K | $1.3M |
| 97 | Fresh Start for Women | Philanthropy, Voluntarism & Grantmaking | $259K | $925K |
| 98 | Thruston-philpoy Volunteer Fire Department INC | Unclassified | $240K | $89K |
| 99 | Owensboro Ice Foundation INC | Recreation & Sports | $233K | $125K |
| 100 | Owensboro Daviess County Humane Society INC | Unclassified | $230K | $257K |