| 1 | University of Dubuque | Education | $184.2M | $446.9M |
| 2 | The Finley Hospital | Health Care | $182.0M | $257.3M |
| 3 | Community Foundation of Greater Dubuque | Philanthropy, Voluntarism & Grantmaking | $89.9M | $165.4M |
| 4 | Loras College | Unclassified | $85.6M | $112.3M |
| 5 | Dutrac Community Credit Union | Public & Societal Benefit | $62.5M | $1.2B |
| 6 | Dubuque Racing Association Ltd | Unclassified | $59.4M | $93.8M |
| 7 | Medical Associates Clinic Health Plan of Wisconsin | Unclassified | $56.7M | $7.9M |
| 8 | Clarke University of Dubuque Iowa | Education | $51.2M | $73.5M |
| 9 | Stonehill Franciscan Services | Human Services | $31.3M | $47.0M |
| 10 | Hills and Dales Child Development Center | Health Care | $28.1M | $13.4M |
| 11 | Butler Family Foundation | Philanthropy, Voluntarism & Grantmaking | $23.4M | $38.0M |
| 12 | Crescent Community Health Center | Health Care | $22.7M | $26.3M |
| 13 | Hillcrest Family Services | Human Services | $18.2M | $22.7M |
| 14 | Hospice of Dubuque Foundation | Human Services | $16.3M | $18.3M |
| 15 | Dubuque Symphony Orchestra | Unclassified | $12.9M | $9.6M |
| 16 | Cottingham and Butler Insurance Service INC Employee Benefits Trus | Unclassified | $11.5M | $2.2M |
| 17 | Hospice of Dubuque | Unclassified | $11.0M | $6.1M |
| 18 | Bethany Home | Health Care | $10.4M | $14.6M |
| 19 | Area Residential Care INC | Human Services | $10.0M | $14.4M |
| 20 | Aim Credit Union | Unclassified | $9.9M | $188.3M |
| 21 | Northeast Iowa Area Agency on Aging INC | Human Services | $9.7M | $4.0M |
| 22 | Believers Stewardship Services INC | Philanthropy, Voluntarism & Grantmaking | $9.0M | $23.4M |
| 23 | Emmaus Bible College | Unclassified | $8.4M | $12.1M |
| 24 | Dubuque County Historical Society | Arts, Culture & Humanities | $7.7M | $38.8M |
| 25 | Dubuque Golf and Country Club | Unclassified | $6.8M | $9.5M |
| 26 | Dubuque Community Y | Human Services | $6.8M | $8.1M |
| 27 | Butler Childrens Garden Foundation INC | Philanthropy, Voluntarism & Grantmaking | $6.1M | $9.0M |
| 28 | Jamie Barwick Hills and Dales Foundation | Human Services | $5.2M | $12.1M |
| 29 | Catholic Charities of the Archdioce Se of Dubuque Iowa | Unclassified | $4.9M | $18.4M |
| 30 | Opening Doors | Unclassified | $4.7M | $7.2M |
| 31 | Alexander D Larson Gift Fund 00670000 | Philanthropy, Voluntarism & Grantmaking | $4.3M | $7.7M |
| 32 | A Y McDonald Mfg Co Charitable Foundation | Unclassified | $4.0M | $8.6M |
| 33 | The Dubuque Visiting Nurse Association | Unclassified | $3.8M | $4.5M |
| 34 | Dubuque Area Convention and Visitors Bureau | Community Improvement & Capacity Building | $2.9M | $2.4M |
| 35 | Hennessey Charitable Trust | Unclassified | $2.8M | $82.2M |
| 36 | Stonehill Benevolent Foundation | Human Services | $2.7M | $9.7M |
| 37 | Delaware Dubuque and Jackson County Regional Transit Authority | Public & Societal Benefit | $2.6M | $4.7M |
| 38 | Finley Health Foundation INC | Health Care | $2.6M | $20.8M |
| 39 | Legion-aires Drum and Bugle Corps | Arts, Culture & Humanities | $2.4M | $1.5M |
| 40 | Dubuque Initiatives | Community Improvement & Capacity Building | $2.4M | $12.7M |
| 41 | Boys and Girls Club of Greater Dubu Que | Youth Development | $2.4M | $13.1M |
| 42 | Dubuque County Fair Assn. | Unclassified | $2.2M | $1.3M |
| 43 | Dubuque Dream Center INC | Youth Development | $2.2M | $3.0M |
| 44 | International Union United Auto Aerospace & Agricultural Workers | Unclassified | $2.1M | $1.3M |
| 45 | Greater Dubuque Development Corporation | Unclassified | $2.0M | $1.5M |
| 46 | St. Mark Youth Enrichment | Unclassified | $2.0M | $1.2M |
| 47 | James & Melita a McDonough Foundation | Philanthropy, Voluntarism & Grantmaking | $1.9M | $8.7M |
| 48 | Dubuque Museum of Art | Arts, Culture & Humanities | $1.9M | $6.0M |
| 49 | Dupaco Rw Hoefer Foundation | Philanthropy, Voluntarism & Grantmaking | $1.9M | $9.2M |
| 50 | Eastern Iowa Regional Housing Corporation | Unclassified | $1.9M | $4.6M |
| 51 | Dubuque Mercy Health Foundation | Health Care | $1.7M | $8.4M |
| 52 | Dubuque Humane Society | Animal-Related | $1.6M | $4.1M |
| 53 | Society of St. Vincent de Paul Particular Council City of Dubuque | Unclassified | $1.6M | $1.2M |
| 54 | Dubuque Area Chamber of Commerce | Unclassified | $1.6M | $1.2M |
| 55 | Schmitt Island Development Corporation | Public & Societal Benefit | $1.4M | $800K |
| 56 | United Way of Dubuque Area Tri- States | Philanthropy, Voluntarism & Grantmaking | $1.4M | $2.0M |
| 57 | Gantz Foundation Charit Tr | Unclassified | $1.4M | $1.4M |
| 58 | Catholic Charities Foundation | Unclassified | $1.4M | $12.9M |
| 59 | Boy Scouts of America | Unclassified | $1.4M | $4.2M |
| 60 | Four Mounds Foundation | Arts, Culture & Humanities | $1.3M | $699K |
| 61 | Dubuque Arboretum Association INC | Environment | $1.2M | $3.2M |
| 62 | North American Invasive Species Mangement Association | Social Science | $1.1M | $395K |
| 63 | Habitat for Humanity International INC | Unclassified | $1.0M | $1.3M |
| 64 | Alverno Apartments INC | Housing & Shelter | $1.0M | $2.9M |
| 65 | Tri-state Independent Blind Society INC | Human Services | $978K | $2.4M |
| 66 | Share International | Human Services | $961K | $1.5M |
| 67 | Creative Adventure Lab INC | Arts, Culture & Humanities | $955K | $662K |
| 68 | Mt Pleasant Home | Unclassified | $905K | $4.9M |
| 69 | Dubuque Dream Center Academy | Education | $900K | $0 |
| 70 | Ecs Ministries | Religion-Related | $857K | $1.7M |
| 71 | Pigeon Art Center INC | Arts, Culture & Humanities | $850K | $585K |
| 72 | Community Solutions of Eastern Iowa | Public & Societal Benefit | $849K | $189K |
| 73 | The Grand Opera House | Arts, Culture & Humanities | $820K | $3.5M |
| 74 | International Brotherhood of Electrical Workers | Unclassified | $790K | $305K |
| 75 | Marys Inn | Unclassified | $772K | $952K |
| 76 | Dubuque County Early Childhood | Community Improvement & Capacity Building | $771K | $261K |
| 77 | Two by Two Character Development | Youth Development | $769K | $664K |
| 78 | Spahn & Rose Lumber Co Charitable Foundation | Unclassified | $734K | $1.8M |
| 79 | Linwood Cemetery Association | Unclassified | $688K | $2.6M |
| 80 | River Valley Initiative Foundation | Human Services | $671K | $1.3M |
| 81 | Holscher Family Charitable Trust | Philanthropy, Voluntarism & Grantmaking | $646K | $2.6M |
| 82 | The Pregnancy Center | Health Care | $620K | $2.6M |
| 83 | Dubuque County Firefighters Assn. | Public Safety, Disaster Preparedness & Relief | $612K | $427K |
| 84 | Herrig Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $544K | $633K |
| 85 | Dubuque Shooting Society | Unclassified | $541K | $1.4M |
| 86 | Manasseh House | Housing & Shelter | $528K | $3.8M |
| 87 | Dubuque County Right to Life | Civil Rights, Social Action & Advocacy | $468K | $768K |
| 88 | Molo Fam Chrtble Tr 11132000 | Philanthropy, Voluntarism & Grantmaking | $466K | $1.1M |
| 89 | Dubuque Main Street Ltd | Community Improvement & Capacity Building | $463K | $289K |
| 90 | Mississippi Driftless Regional Foundation | Community Improvement & Capacity Building | $458K | $327K |
| 91 | Presentation Lantern | Unclassified | $451K | $3.6M |
| 92 | Fountain of Youth Program | Employment | $447K | $505K |
| 93 | Westminster Presbyterian Church Foundation of Dubuque Iowa | Unclassified | $435K | $1.5M |
| 94 | Dubuque Soccer Club | Recreation & Sports | $427K | $258K |
| 95 | Pauline Stolteben Foundation Trust | Unclassified | $426K | $1.5M |
| 96 | Bell Tower Production & Dinner Theater | Arts, Culture & Humanities | $416K | $410K |
| 97 | Dubuque County Hockey Association | Recreation & Sports | $406K | $89K |
| 98 | Dubuque Montessori School INC | Unclassified | $402K | $356K |
| 99 | Resourcesunite | Human Services | $392K | $223K |
| 100 | Bethany Home Foundation | Health Care | $392K | $5.1M |