| 1 | Wells Fargo & Company Employee Benefit Trust | Unclassified | $2.7B | $14.1M |
| 2 | Central Iowa Hospital Corporation | Health Care | $2.4B | $1.8B |
| 3 | Catholic Health Initiatives-iowa Corp. | Health Care | $978.7M | $1.1B |
| 4 | Drake University | Education | $363.3M | $628.3M |
| 5 | Community Fdn of Greater des Moines | Philanthropy, Voluntarism & Grantmaking | $319.8M | $918.7M |
| 6 | Mercy Clinics INC | Health Care | $203.3M | $275.0M |
| 7 | Lincoln National Corporation Voluntary Employee Bene Assoc Tr | Unclassified | $178.0M | $19.6M |
| 8 | Des Moines University Osteopathic Medical Center | Education | $151.0M | $522.1M |
| 9 | Wells Fargo & Company Short Term Disability Plan & Trust | Mutual & Membership Benefit | $122.0M | $23.1M |
| 10 | Grand View University | Education | $79.6M | $116.5M |
| 11 | The Principal Trust for Health Bens for Ees and Non-medicare Elig Rets | Unclassified | $77.2M | $201.7M |
| 12 | Lifeserve Blood Center | Health Care | $75.1M | $122.4M |
| 13 | City of Raleigh North Carolina Medical Benefits Trust | Unclassified | $66.5M | $33.7M |
| 14 | Primary Health Care INC | Health Care | $62.5M | $36.7M |
| 15 | National Pork Producers Council | Unclassified | $60.9M | $39.1M |
| 16 | Iowa Health Foundation | Health Care | $58.7M | $223.4M |
| 17 | Food Bank of Iowa | Food, Agriculture & Nutrition | $50.8M | $45.2M |
| 18 | Richard O Jacobson Foundation INC | Unclassified | $45.0M | $66.7M |
| 19 | P E O Foundation | Education | $44.9M | $167.8M |
| 20 | Impact Community Action Partnership INC | Unclassified | $43.1M | $2.5M |
| 21 | Lutheran Services in Iowa INC | Human Services | $42.1M | $26.2M |
| 22 | Mercy Medical Center-newton | Health Care | $41.6M | $59.2M |
| 23 | Des Moines Performing Arts | Community Improvement & Capacity Building | $39.1M | $51.0M |
| 24 | Plumbers & Steamfitters H & W Trust Iowa State Bank Ttee | Unclassified | $37.9M | $98.0M |
| 25 | Iowa Natural Heritage Foundation | Environment | $37.6M | $160.4M |
| 26 | Pds Veba Tr | Mutual & Membership Benefit | $37.4M | $85K |
| 27 | United Way of Central Iowa | Unclassified | $33.9M | $38.6M |
| 28 | Habitat for Humanity International INC | Housing & Shelter | $33.7M | $43.7M |
| 29 | Seidler Foundation | Unclassified | $33.1M | $88.0M |
| 30 | Thomas and Linda Koehn Foundation | Unclassified | $32.4M | $64.2M |
| 31 | Welfare Tr for Centerra Group LLC and Affiliates | Employment | $32.3M | $356K |
| 32 | Oklahoma Gas and Electric Company Employees Medical Benefit Trust | Unclassified | $32.1M | $722K |
| 33 | Eyerly-ball Community Mental Health Services | Mental Health & Crisis Intervention | $32.0M | $13.2M |
| 34 | Cooper-hansen Foundation | Education | $31.8M | $28.4M |
| 35 | Visiting Nurse Services of Iowa | Health Care | $30.4M | $29.6M |
| 36 | Operating Engineers Local 234 Health and Welfare Fund | Unclassified | $29.9M | $67.3M |
| 37 | Ukpeagvik Inupiat Corp Voluntary Employees Beneficiary Assoc | Mutual & Membership Benefit | $27.5M | $7.9M |
| 38 | Emc Insurance Foundation | Unclassified | $27.4M | $20.8M |
| 39 | Shell Oil Dental Assistance Plan | Unclassified | $26.6M | $7.8M |
| 40 | Sehgal Foundation | Philanthropy, Voluntarism & Grantmaking | $26.2M | $57.1M |
| 41 | Orchard Place | Mental Health & Crisis Intervention | $22.8M | $12.9M |
| 42 | Mercy Health Network INC | Unclassified | $22.4M | $439.3M |
| 43 | Mercy Foundation of des Moines Iowa | Health Care | $21.8M | $91.4M |
| 44 | Iowa Life and Health Insurance Guaranty Association | Unclassified | $20.6M | $24.4M |
| 45 | Portland General Electric Company Veba for Health Reimbursement Arra | Mutual & Membership Benefit | $20.4M | $16.5M |
| 46 | Institute for Community Alliances | Housing & Shelter | $19.3M | $3.5M |
| 47 | Iowa Hospital Association | Unclassified | $18.5M | $19.3M |
| 48 | Iowa Legal Aid | Crime & Legal-Related | $18.2M | $19.8M |
| 49 | Mercy College of Health Sciences | Religion-Related | $18.2M | $79.0M |
| 50 | Iowa Jag INC | Youth Development | $18.1M | $10.2M |
| 51 | Iowa State Education Assn. | Unclassified | $16.9M | $19.5M |
| 52 | Hope Ministries | Human Services | $16.6M | $39.1M |
| 53 | Golf Charitable Foundation of Greater des Moines | Philanthropy, Voluntarism & Grantmaking | $16.4M | $2.8M |
| 54 | Luther Park Health Center INC | Unclassified | $16.4M | $13.9M |
| 55 | Young Mens Christian Association of Greater des Moines | Unclassified | $16.1M | $54.4M |
| 56 | Kyle J and Sharon Krause Family Foundation | International, Foreign Affairs & National Security | $15.8M | $121.5M |
| 57 | The Wellmark Foundation | Unclassified | $15.5M | $79.8M |
| 58 | Easter Seal Society of Iowa INC | Unclassified | $15.4M | $17.5M |
| 59 | Children and Families of Iowa | Human Services | $15.2M | $26.4M |
| 60 | Anna K Meredith Endowment Trust | Arts, Culture & Humanities | $14.9M | $56.6M |
| 61 | Iowa State Fair Foundation | Recreation & Sports | $14.8M | $26.3M |
| 62 | Edmundson Art Foundation INC | Arts, Culture & Humanities | $14.6M | $175.7M |
| 63 | Animal Rescue League of Iowa Incorporated | Animal-Related | $13.2M | $13.7M |
| 64 | Meredith Corporation Foundation | Unclassified | $13.2M | $23.6M |
| 65 | Grand View Christian School Association INC | Education | $13.1M | $15.6M |
| 66 | Greater des Moines Botanical Garden | Environment | $12.8M | $31.4M |
| 67 | Bookey Family Foundation | Philanthropy, Voluntarism & Grantmaking | $12.6M | $7.2M |
| 68 | Blank Park Zoo Foundation INC | Animal-Related | $12.5M | $28.5M |
| 69 | Iowa Jewish Senior Life Center | Health Care | $12.4M | $6.5M |
| 70 | Scottish Rite Park INC | Unclassified | $12.3M | $30.7M |
| 71 | Animal Rescue League of Iowa Foundation | Unclassified | $11.8M | $14.5M |
| 72 | Peo Sisterhood International Chapter | Unclassified | $11.8M | $95.1M |
| 73 | Edwin T Meredith Foundation | Unclassified | $11.6M | $22.7M |
| 74 | Neighborhood Finance Corporation | Housing & Shelter | $11.4M | $114.2M |
| 75 | P E O Educational Loan Fund | Education | $11.1M | $99.2M |
| 76 | Calvin Community | Unclassified | $11.1M | $5.6M |
| 77 | Iowa Public Radio INC | Arts, Culture & Humanities | $11.0M | $16.0M |
| 78 | Invest Dsm | Housing & Shelter | $10.9M | $29.0M |
| 79 | Central Iowa Sheet Metal Health Fund Trust | Unclassified | $10.9M | $15.6M |
| 80 | Iowa Confluence Water Trails | Environment | $10.5M | $52.1M |
| 81 | Principal Trust for Life Insurance Benefits for Employees | Mutual & Membership Benefit | $10.2M | $42.8M |
| 82 | Science Center of Iowa | Arts, Culture & Humanities | $10.0M | $48.5M |
| 83 | Greater des Moines Partnership | Community Improvement & Capacity Building | $9.8M | $10.0M |
| 84 | University of Colorado Childrens Dia | Unclassified | $9.5M | $8.2M |
| 85 | Bravo Greater des Moines INC | Arts, Culture & Humanities | $9.4M | $11.3M |
| 86 | Oklahoma Gas & Electric Company Medical Benefit Tr | Unclassified | $9.2M | $35.3M |
| 87 | Susan J Glazer Foundation | Unclassified | $8.7M | $5.5M |
| 88 | Intermountain Power Service Corporation Veba Trust | Unclassified | $8.6M | $83.1M |
| 89 | Hospice of Central Iowa Foundation | Human Services | $8.6M | $17.3M |
| 90 | Bridges of Iowa | Mental Health & Crisis Intervention | $8.6M | $8.9M |
| 91 | Greater des Moines Convention and Visitors Bureau INC | Unclassified | $8.5M | $5.4M |
| 92 | Midamerican Energy Foundation | Philanthropy, Voluntarism & Grantmaking | $8.1M | $40.2M |
| 93 | Iowa Primary Care Association INC | Unclassified | $8.0M | $3.7M |
| 94 | Anawim Housing | Unclassified | $7.9M | $12.7M |
| 95 | Des Moines Area Religious Council | Human Services | $7.9M | $10.7M |
| 96 | Principal Financial Group Foundation INC | Unclassified | $7.7M | $204.1M |
| 97 | Catholic Council for Social Concern INC | Unclassified | $7.7M | $7.9M |
| 98 | Wakonda Club | Unclassified | $7.3M | $15.5M |
| 99 | Foundation for Children and Families of Iowa | Unclassified | $7.2M | $17.2M |
| 100 | Royal Ten Cate U S a INC Health and Welfare Trust | Unclassified | $7.0M | $83K |