| 1 | Iowa State University Foundation | Education | $435.2M | $2.0B |
| 2 | Youth and Shelter Services INC | Human Services | $27.3M | $40.6M |
| 3 | Mainstream Living INC Stuart Ambrose | Human Services | $24.1M | $15.3M |
| 4 | Practical Farmers of Iowa | Unclassified | $22.4M | $13.6M |
| 5 | Krell Institute INC | Education | $20.0M | $3.8M |
| 6 | Northcrest INC | Human Services | $19.4M | $89.2M |
| 7 | Resurrection University | Education | $18.8M | $61.1M |
| 8 | Mary Greeley Medical Center Foundation | Health Care | $17.7M | $19.5M |
| 9 | Iowa State University Research Park Corp. | Education | $14.2M | $100.4M |
| 10 | Friendship Ark INC | Housing & Shelter | $6.9M | $7.1M |
| 11 | Iowa Cattlemens Association | Unclassified | $6.0M | $3.9M |
| 12 | Iowa State University 1993 Trust | Education | $4.7M | $11.4M |
| 13 | Miller Endowment Incorporated | Education | $4.6M | $19.5M |
| 14 | Iowa Youth Athletic Foundation | Recreation & Sports | $4.5M | $34.9M |
| 15 | Ames Regional Economic Alliance | Unclassified | $4.0M | $3.2M |
| 16 | 4-H Clubs & Affiliated 4-H Organizations | Unclassified | $3.7M | $21.9M |
| 17 | Iowa State University Alumni Association | Education | $3.7M | $20.9M |
| 18 | Iowa Beef Cattle Producers Association | Unclassified | $3.4M | $3.1M |
| 19 | Ames Golf and Country Club | Unclassified | $3.2M | $2.0M |
| 20 | Boys and Girls Clubs of Story County INC | Youth Development | $3.1M | $8.7M |
| 21 | Shelter Housing Corporation | Housing & Shelter | $3.1M | $8.0M |
| 22 | Burke Family Foundation | Philanthropy, Voluntarism & Grantmaking | $2.5M | $5.5M |
| 23 | Ames Community Pre School Center | Human Services | $2.5M | $2.9M |
| 24 | Ames Convention & Visitors Bureau | Unclassified | $2.4M | $3.3M |
| 25 | United Way of Story County INC | Philanthropy, Voluntarism & Grantmaking | $2.4M | $4.4M |
| 26 | Iowa Cattlemens Association | Unclassified | $2.4M | $3.1M |
| 27 | Daniel J & Ann L Krumm Charitable Trust | Unclassified | $2.3M | $4.5M |
| 28 | Youth and Shelter Services Foundation INC | Unclassified | $2.0M | $9.5M |
| 29 | Northcrest Foundation INC | Philanthropy, Voluntarism & Grantmaking | $2.0M | $4.9M |
| 30 | Overflow Thrift Store INC | Human Services | $2.0M | $2.7M |
| 31 | F William Beckwith & Leola I Beckwith Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $1.9M | $6.7M |
| 32 | Cornerstone Counseling Center of Ames INC | Mental Health & Crisis Intervention | $1.7M | $234K |
| 33 | Iowa Sports Foundation | Unclassified | $1.6M | $1.2M |
| 34 | Committee for Agricultural Development INC | Unclassified | $1.5M | $18.1M |
| 35 | Assault Care Center Extending Shelter & Support | Mental Health & Crisis Intervention | $1.4M | $1.2M |
| 36 | University Community Childcare INC | Human Services | $1.4M | $1.2M |
| 37 | Becker Family Foundation | Philanthropy, Voluntarism & Grantmaking | $1.4M | $14.6M |
| 38 | Habitat for Humanity International INC | Unclassified | $1.3M | $2.0M |
| 39 | Council for Agricultural Science and Technology | Unclassified | $1.2M | $1.5M |
| 40 | Iowa State Univ Chapter Triangle Fraternity | Unclassified | $1.2M | $10K |
| 41 | Heartland Senior Services | Human Services | $1.2M | $13.0M |
| 42 | Ames Christian School | Education | $1.1M | $1.1M |
| 43 | Happy Doggo | Animal-Related | $1.1M | $874K |
| 44 | Underwood Family Foundation | Philanthropy, Voluntarism & Grantmaking | $1.1M | $14.8M |
| 45 | 46 1 Fund | Philanthropy, Voluntarism & Grantmaking | $1.1M | $3.6M |
| 46 | Iowa Institute for Cooperatives | Unclassified | $1.1M | $987K |
| 47 | Wings of Refuge | Human Services | $1.1M | $1.6M |
| 48 | Ames Historical Society | Unclassified | $969K | $5.2M |
| 49 | Phi Gamma Delta Fraternity | Unclassified | $954K | $1.3M |
| 50 | Ames Education Foundation | Education | $910K | $1.7M |
| 51 | The Richmond Center | Unclassified | $904K | $693K |
| 52 | Anchored Hope | Religion-Related | $898K | $913K |
| 53 | Delta Tau Delta Fraternity | Unclassified | $883K | $45K |
| 54 | Iowa State Daily Publication | Arts, Culture & Humanities | $877K | $169K |
| 55 | Asphalt Paving Association of Iowa | Unclassified | $868K | $481K |
| 56 | Delta Upsilon International Fraternity INC | Mutual & Membership Benefit | $844K | $1.4M |
| 57 | Farm House Fraternity INC | Unclassified | $767K | $106K |
| 58 | Obria Medical Clinic of Ames | Health Care | $747K | $340K |
| 59 | Women Food and Agriculture Network | Food, Agriculture & Nutrition | $721K | $657K |
| 60 | Alpha Gamma Delta Fraternity | Unclassified | $697K | $122K |
| 61 | Ames Soccer Club | Recreation & Sports | $677K | $441K |
| 62 | Human Restoration Project | Education | $677K | $58K |
| 63 | Gamma Phi Beta Sorority INC | Unclassified | $641K | $56K |
| 64 | Ames Cyclone Aquatics Club | Recreation & Sports | $625K | $134K |
| 65 | Ames Senior Living | Health Care | $603K | $2.9M |
| 66 | Iowa Turkey Federation INC | Unclassified | $599K | $564K |
| 67 | Professionals in Christian Philanthropy | Religion-Related | $593K | $382K |
| 68 | Iowa Limestone Producers Association INC | Unclassified | $571K | $283K |
| 69 | Isu Facilities Corporation | Education | $568K | $43.8M |
| 70 | Together for Story County INC | Religion-Related | $567K | $64K |
| 71 | Boone County Economic Growth Corp. | Unclassified | $566K | $1.0M |
| 72 | Alpha Tau Omega Fraternity | Unclassified | $564K | $86K |
| 73 | Alpha Delta Pi Building Corporation Pi Chapter | Unclassified | $545K | $1.7M |
| 74 | Ames Public Library Friends Foundation | Education | $532K | $3.5M |
| 75 | Theta Chi Fraternity INC | Unclassified | $528K | $114K |
| 76 | Gamm Phi Beta Sorority INC | Unclassified | $523K | $899K |
| 77 | Epsilon Chapter of Tau Kappa Epsilon Alumni Corporation | Education | $517K | $3.1M |
| 78 | National Student Exchange INC | Human Services | $502K | $1.3M |
| 79 | Hammond Family Foundation | Philanthropy, Voluntarism & Grantmaking | $502K | $488K |
| 80 | Grace Foundation | Unclassified | $501K | $766K |
| 81 | Ames Chi Omega Alumnae Association | Education | $498K | $1.4M |
| 82 | Good Neighbor Emergency Assistance INC | Human Services | $470K | $189K |
| 83 | Arc of Iowa | Unclassified | $442K | $673K |
| 84 | Octagon Center for the Arts | Arts, Culture & Humanities | $441K | $659K |
| 85 | Theta Delta Chi INC | Unclassified | $429K | $16K |
| 86 | Ames Minor Hockey Association | Recreation & Sports | $428K | $182K |
| 87 | Bailing Out Benji | Animal-Related | $409K | $516K |
| 88 | Theta Chi Corporation | Unclassified | $365K | $539K |
| 89 | Amercian Legion | Unclassified | $363K | $426K |
| 90 | Iowa State University Agricultural Endowment | Education | $355K | $4.6M |
| 91 | Phi Delta Theta Fraternity | Unclassified | $353K | $23K |
| 92 | Sigma Chi Fraternity | Unclassified | $347K | $3.7M |
| 93 | Prairie Rivers of Iowa Resource Conservation and Development INC | Education | $334K | $155K |
| 94 | Iowa Wildlife Center | Animal-Related | $324K | $684K |
| 95 | Raising Readers in Story County | Human Services | $317K | $814K |
| 96 | Nami Central Iowa | Unclassified | $315K | $120K |
| 97 | Kappa Sigma Fraternity | Unclassified | $313K | $2K |
| 98 | Food at First | Human Services | $313K | $1.2M |
| 99 | Alpha Chi Omega House Corp for Delta Nu Chapter | Education | $306K | $1.3M |
| 100 | Oscar Romero Tr | Housing & Shelter | $304K | $670K |