| 1 | University of Denver | Education | $1.4B | $2.2B |
| 2 | The Colorado Health Foundation | Health Care | $968.3M | $2.9B |
| 3 | Anschutz Foundation | Philanthropy, Voluntarism & Grantmaking | $868.0M | $1.9B |
| 4 | Saint Joseph Hospital | Health Care | $649.1M | $1.0B |
| 5 | Denver Health Medical Plan INC | Unclassified | $572.8M | $89.0M |
| 6 | Charter Fund INC | Philanthropy, Voluntarism & Grantmaking | $486.3M | $720.3M |
| 7 | Denver Foundation | Philanthropy, Voluntarism & Grantmaking | $464.2M | $1.3B |
| 8 | University of Colorado Foundation | Education | $456.1M | $3.5B |
| 9 | Rose Community Foundation | Philanthropy, Voluntarism & Grantmaking | $442.4M | $399.6M |
| 10 | National Jewish Health | Health Care | $428.6M | $414.0M |
| 11 | Zoma Foundation | Philanthropy, Voluntarism & Grantmaking | $312.5M | $529.5M |
| 12 | Credit Unions Chartered in the State of Colorado | Unclassified | $240.9M | $2.1B |
| 13 | Regis University | Education | $214.3M | $219.9M |
| 14 | Denver Center for the Performing Arts | Arts, Culture & Humanities | $174.3M | $105.4M |
| 15 | Mental Health Center of Denver | Mental Health & Crisis Intervention | $148.8M | $111.4M |
| 16 | Daniels Fund | Philanthropy, Voluntarism & Grantmaking | $148.7M | $1.4B |
| 17 | Denver Dumb Friends League | Animal-Related | $131.3M | $180.3M |
| 18 | Colorado Trust | Philanthropy, Voluntarism & Grantmaking | $130.3M | $548.0M |
| 19 | Denver School of Science and Technology INC | Education | $123.2M | $68.7M |
| 20 | Colorado Coalition for the Homeless | Human Services | $116.9M | $203.5M |
| 21 | Community Firm | Crime & Legal-Related | $102.2M | $15.6M |
| 22 | Strive Preparatory Schools | Education | $92.7M | $37.4M |
| 23 | Rocky Mountain Planned Parenthood INC | Health Care | $90.6M | $75.8M |
| 24 | American Indian College Fund | Education | $90.5M | $215.1M |
| 25 | Donor Alliance INC | Health Care | $89.9M | $124.1M |
| 26 | Energy Outreach Colorado | Recreation & Sports | $86.1M | $50.6M |
| 27 | Rocky Mountain Human Services | Human Services | $84.5M | $44.6M |
| 28 | International Medical Relief | Health Care | $76.6M | $1.7M |
| 29 | Pipe Industry Insurance Fund | Unclassified | $68.0M | $52.3M |
| 30 | Denver Rescue Mission | Human Services | $66.0M | $67.3M |
| 31 | Rocky Mountain Mutual Housing Association INC | Housing & Shelter | $62.4M | $102.6M |
| 32 | Dsst Public Schools Foundation | Education | $61.1M | $61.0M |
| 33 | Denver Zoological Foundation INC | Animal-Related | $60.4M | $32.6M |
| 34 | Colorado Health Benefit Exchange | Health Care | $60.3M | $55.5M |
| 35 | The Colorado Museum of Natural History | Arts, Culture & Humanities | $60.3M | $27.7M |
| 36 | Schmitz Family Foundation | Philanthropy, Voluntarism & Grantmaking | $58.5M | $55.5M |
| 37 | Clay Mathematics Institute INC | Science & Technology | $58.3M | $49.3M |
| 38 | Colorado Academy | Education | $57.7M | $133.7M |
| 39 | Galena Foundation | Philanthropy, Voluntarism & Grantmaking | $56.9M | $39.0M |
| 40 | The Symphony Fund | Arts, Culture & Humanities | $56.2M | $98.0M |
| 41 | Nurse Family Partnership | Health Care | $54.6M | $118.1M |
| 42 | Caring for Denver Foundation | Mental Health & Crisis Intervention | $52.7M | $108.6M |
| 43 | Western Stock Show Association | Recreation & Sports | $51.9M | $111.5M |
| 44 | Mandarich Family Foundation | Unclassified | $50.9M | $60.2M |
| 45 | United States Meat Export Federation INC | Unclassified | $50.6M | $23.2M |
| 46 | The American University of Paris INC | Unclassified | $49.1M | $86.3M |
| 47 | American Water Works Association | Public & Societal Benefit | $48.0M | $52.0M |
| 48 | Louis Calder Foundation | Unclassified | $47.8M | $194.2M |
| 49 | Denver Metro Convention & Visitors Bureau | Unclassified | $46.8M | $62.9M |
| 50 | Mercy Housing INC | Housing & Shelter | $46.5M | $502.7M |
| 51 | Community Development Institute Head Start | Education | $46.2M | $2.8M |
| 52 | Water Research Foundation | Environment | $45.7M | $57.1M |
| 53 | Kipp Colorado Schools | Education | $45.7M | $22.5M |
| 54 | Mile High United Way INC | Philanthropy, Voluntarism & Grantmaking | $44.0M | $80.2M |
| 55 | Denver Botanic Garden INC | Environment | $43.9M | $21.4M |
| 56 | Denver Art Museum INC | Arts, Culture & Humanities | $43.8M | $179.4M |
| 57 | International Development Enterprises | International, Foreign Affairs & National Security | $43.4M | $16.8M |
| 58 | Girl Scouts of Colorado | Youth Development | $42.1M | $43.5M |
| 59 | Society of Family Planning | Community Improvement & Capacity Building | $41.8M | $39.8M |
| 60 | Catholic Charities and Community Services of the Archdiocese of Den | Human Services | $41.6M | $40.4M |
| 61 | Adolph Coors Foundation | Unclassified | $40.8M | $206.6M |
| 62 | P3 Foundation INC | Human Services | $40.4M | $1.3B |
| 63 | Association of Operating Room Nurses INC | Unclassified | $39.4M | $49.2M |
| 64 | Temple Hoyne Buell Foundation | Unclassified | $39.1M | $477.1M |
| 65 | Habitat for Humanity of Metro Denver INC | Unclassified | $39.0M | $75.4M |
| 66 | Hospice of Metro Denver Incorporated | Human Services | $38.3M | $73.6M |
| 67 | Schlessman Foundation INC | Unclassified | $38.2M | $86.4M |
| 68 | Mercy Housing California | Housing & Shelter | $37.8M | $272.0M |
| 69 | Elevation Community Land Trust | Housing & Shelter | $37.7M | $102.0M |
| 70 | Impact Charitable | Philanthropy, Voluntarism & Grantmaking | $37.3M | $40.7M |
| 71 | Colorado Health Network INC | Diseases, Disorders & Medical Disciplines | $36.7M | $17.3M |
| 72 | Lutheran Social Service of Colorado | Unclassified | $35.7M | $11.2M |
| 73 | Nextfifty Initiative | Health Care | $34.6M | $263.8M |
| 74 | Cable Center | Education | $34.5M | $54.9M |
| 75 | Gates Family Foundation | Unclassified | $31.6M | $430.9M |
| 76 | The Denver Health and Hospitals Foundation | Unclassified | $30.7M | $37.0M |
| 77 | Graland Country Day | Education | $30.5M | $34.7M |
| 78 | Fujitsu Group Health Benefit Plan Tr | Unclassified | $29.7M | $4.0M |
| 79 | International Association for the Study of Lung Cancer | Community Improvement & Capacity Building | $29.2M | $35.6M |
| 80 | Credit Unions Chartered in the State of Colorado | Unclassified | $28.3M | $410.1M |
| 81 | Unite America INC | Civil Rights, Social Action & Advocacy | $28.2M | $7.0M |
| 82 | Mercy Housing Lakefront | Housing & Shelter | $28.1M | $70.1M |
| 83 | Dmns Foundation | Arts, Culture & Humanities | $26.9M | $177.0M |
| 84 | Laradon Hall Society for Exceptional Children and Adults | Human Services | $26.3M | $25.1M |
| 85 | Boys and Girls Clubs of Metro Denver INC | Unclassified | $26.3M | $82.8M |
| 86 | Jewishcolorado | Philanthropy, Voluntarism & Grantmaking | $26.2M | $84.3M |
| 87 | Metropolitan State University of Denver Foundation INC | Education | $25.8M | $59.0M |
| 88 | Corkins Family Foundation | Philanthropy, Voluntarism & Grantmaking | $25.6M | $10.4M |
| 89 | United States Center for Safesport | Recreation & Sports | $25.5M | $17.8M |
| 90 | Colorado Legal Services | Crime & Legal-Related | $25.0M | $18.9M |
| 91 | Wedontwaste INC | Food, Agriculture & Nutrition | $24.7M | $13.4M |
| 92 | Pikes Peak Hospice and Palliative Care INC | Unclassified | $24.5M | $23.0M |
| 93 | Rocky Mountain Public Media INC | Arts, Culture & Humanities | $24.5M | $69.5M |
| 94 | Alpha Phi International Fraternity INC | Unclassified | $24.5M | $72.9M |
| 95 | Boettcher Foundation | Philanthropy, Voluntarism & Grantmaking | $24.4M | $279.0M |
| 96 | Gill Foundation | Unclassified | $24.2M | $194.3M |
| 97 | Colorado Enterprise Fund INC | Community Improvement & Capacity Building | $24.0M | $78.7M |
| 98 | Pathways Hospice | Human Services | $23.9M | $35.4M |
| 99 | National Environmental Health | Unclassified | $23.8M | $7.9M |
| 100 | Young Mens Christian Association of Metropolitan Denver | Human Services | $23.8M | $32.6M |