| 1 | Five Star Credit Union | Unclassified | $93.5M | $1.2B |
| 2 | Wiregrass Foundation | Philanthropy, Voluntarism & Grantmaking | $43.3M | $114.6M |
| 3 | Wiregrass Rehabilitation Center INC | Employment | $12.7M | $14.9M |
| 4 | Spectracare Health Systems INC | Mental Health & Crisis Intervention | $12.2M | $8.4M |
| 5 | Southeast Education INC | Unclassified | $9.7M | $23.7M |
| 6 | Dothan Country Club | Recreation & Sports | $7.6M | $13.4M |
| 7 | Southeast Alabama Youth Services Incorporated | Human Services | $6.0M | $5.5M |
| 8 | Dothan-houston County Intellectual Disabilites Board INC | Human Services | $5.8M | $9.7M |
| 9 | Wiregrass Pet Rescue and Adoption Center INC | Animal-Related | $5.5M | $10.9M |
| 10 | Dove Family Foundation | Unclassified | $5.0M | $20.6M |
| 11 | Our Community INC | Housing & Shelter | $4.4M | $13.4M |
| 12 | John H Watson Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $4.3M | $32.8M |
| 13 | National Peanut Festival Assn INC | Unclassified | $3.7M | $13.8M |
| 14 | Family Services Center | Human Services | $3.5M | $1.9M |
| 15 | Wiregrass United Way INC | Philanthropy, Voluntarism & Grantmaking | $3.2M | $3.7M |
| 16 | Boy Scouts of America | Unclassified | $2.7M | $2.8M |
| 17 | Wiregrass Area Food Bank INC | Food, Agriculture & Nutrition | $2.3M | $3.9M |
| 18 | Dothan Area Chamber of Commerce | Unclassified | $2.2M | $3.7M |
| 19 | Inlet Beach Water System INC | Unclassified | $2.1M | $7.2M |
| 20 | Emmanuel Christian School Incorporated | Education | $1.9M | $1.6M |
| 21 | Dothan Houston County Library System | Unclassified | $1.8M | $5.1M |
| 22 | Dothan Area Convention & Vistors Bureau INC | Unclassified | $1.8M | $5.0M |
| 23 | Dothan Rescue Mission | Human Services | $1.5M | $3.0M |
| 24 | Habitat for Humanity International INC | Housing & Shelter | $1.5M | $2.1M |
| 25 | Southeast Alabama Medical Centerfoundation | Health Care | $1.4M | $4.6M |
| 26 | National Association of Rc & D Councils INC | Environment | $1.3M | $53K |
| 27 | The Ordinary People Society INC | Human Services | $1.3M | $501K |
| 28 | Wiregrass Hope Group INC | Unclassified | $1.3M | $2.2M |
| 29 | Westgate Christian School | Education | $1.2M | $405K |
| 30 | Boys and Girls Club of the Wiregrass INC | Youth Development | $1.2M | $1.2M |
| 31 | Heersink Family Foundation | Philanthropy, Voluntarism & Grantmaking | $1.2M | $1.8M |
| 32 | George C Wallace State Community College Foundation | Philanthropy, Voluntarism & Grantmaking | $1.2M | $12.0M |
| 33 | Southeast Alabama Child Advocacy Center | Human Services | $1.2M | $1.6M |
| 34 | Alabama Peanut Producers Association INC | Unclassified | $1.1M | $2.7M |
| 35 | Ark Dothan INC | Unclassified | $1.1M | $883K |
| 36 | The House of Ruth INC | Unclassified | $1.1M | $1.4M |
| 37 | Community Care Solutions | Health Care | $1.0M | $460K |
| 38 | Quin E Flowers Jr Foundation | Philanthropy, Voluntarism & Grantmaking | $1.0M | $2.6M |
| 39 | Ephphatha Ministries International | Philanthropy, Voluntarism & Grantmaking | $919K | $2.0M |
| 40 | New Beginning Ministries International Incorporated | Religion-Related | $902K | $13.4M |
| 41 | Wiregrass Spay Neuter Alliance | Animal-Related | $884K | $318K |
| 42 | Chrysalis a Home for Girls INC | Human Services | $872K | $1.1M |
| 43 | Dothan Area Botanical Gardens INC | Environment | $862K | $2.3M |
| 44 | The Wiregrass Museum of Art INC | Arts, Culture & Humanities | $846K | $1.4M |
| 45 | Dothan Landmarks Foundation INC | Unclassified | $829K | $2.3M |
| 46 | Southeast Alabama Community Players INC | Arts, Culture & Humanities | $805K | $902K |
| 47 | Time Youth Dothan INC | Religion-Related | $726K | $505K |
| 48 | Southeast Intervention Group INC | Unclassified | $723K | $514K |
| 49 | Wiregrass Humane Society | Animal-Related | $661K | $1.1M |
| 50 | The Exchange Center for Child Abuse Prevention | Crime & Legal-Related | $643K | $439K |
| 51 | Dothan High School Booster Club INC | Education | $614K | $264K |
| 52 | Bnai Brith Wiregrass Senior Housing INC | Housing & Shelter | $561K | $2.9M |
| 53 | Dho Foundation | Health Care | $555K | $306K |
| 54 | Bnai Brith Wiregrass Senior Housing II INC | Housing & Shelter | $533K | $3.3M |
| 55 | Love in Action International Ministries INC | Religion-Related | $532K | $551K |
| 56 | Dothan Association of Realtors INC | Unclassified | $516K | $874K |
| 57 | Southeast Alabama Works INC | Human Services | $508K | $125K |
| 58 | Alabama Dance Works INC | Arts, Culture & Humanities | $505K | $95K |
| 59 | Southeast Alabama Dance Company INC | Unclassified | $505K | $915K |
| 60 | Wiregrass Angel House INC | Mental Health & Crisis Intervention | $496K | $355K |
| 61 | Southern Peanut Farmers Federation | Food, Agriculture & Nutrition | $488K | $387K |
| 62 | Dubois Institute for Entrepreneurship | Education | $487K | $328K |
| 63 | Southeastern Association of Fire Chiefs | Community Improvement & Capacity Building | $472K | $696K |
| 64 | Wiregrass Childrens Home | Human Services | $439K | $1.1M |
| 65 | Benevolent & Protective Order of Elks of the USA | Unclassified | $416K | $513K |
| 66 | Girls Incorporated of Dothan Alabama | Youth Development | $392K | $749K |
| 67 | The Alabama-west Florida United Methodist Development Fund INC | Unclassified | $387K | $10.3M |
| 68 | Porters Group INC | Human Services | $328K | $25K |
| 69 | Dance Masters of America Chapter 33 INC | Arts, Culture & Humanities | $308K | $304K |
| 70 | Dothan Youth Soccer Association INC | Recreation & Sports | $303K | $27K |
| 71 | The White House INC | Human Services | $298K | $15K |
| 72 | The Robert & Carol Flowers Foundation | Philanthropy, Voluntarism & Grantmaking | $291K | $818K |
| 73 | Southeast Alabama Emergency Medical Services Council | Health Care | $279K | $35K |
| 74 | Wiregrass 2-1-1 | Human Services | $278K | $171K |
| 75 | Home Builders Association of Dothan and the Wiregrass Area | Unclassified | $274K | $610K |
| 76 | Five Star Credit Union Foundation | Unclassified | $252K | $1.6M |
| 77 | Azalea Tennis & Swimming Club INC | Unclassified | $248K | $218K |
| 78 | Krewe of Kolosse INC | Philanthropy, Voluntarism & Grantmaking | $246K | $387K |
| 79 | Wired Ministries INC | Religion-Related | $243K | $63K |
| 80 | Wiregrass-houston County Foundation | Education | $231K | $2.1M |
| 81 | Dothan Montessori School | Education | $230K | $817K |
| 82 | Save a Pet Rescue Adoption and Transport INC | Animal-Related | $224K | $692K |
| 83 | Dupree Family Foundation | Education | $217K | $1.4M |
| 84 | Cultural Arts Center INC | Arts, Culture & Humanities | $216K | $103K |
| 85 | Emmanuel Housing Center | Religion-Related | $214K | $587K |
| 86 | Shelk Fischer Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $213K | $1.1M |
| 87 | Dothan Police Foundation INC | Unclassified | $196K | $323K |
| 88 | Dothan-houston County Substance Abuse Partnership INC | Mental Health & Crisis Intervention | $193K | $114K |
| 89 | Warhawgs | Public & Societal Benefit | $191K | $18K |
| 90 | Trial Lawyer Hall of Fame | Arts, Culture & Humanities | $188K | $305K |
| 91 | Dothan Area Chamber Foundation | Education | $188K | $121K |
| 92 | Alabama Gastroenterological Society INC | Employment | $167K | $228K |
| 93 | Dothan National League INC | Recreation & Sports | $167K | $145K |
| 94 | Jerry Spencer Ministries INC | Religion-Related | $165K | $51K |
| 95 | Kitty Kottage | Animal-Related | $161K | $11K |
| 96 | Empact Southeast Alabama INC | Philanthropy, Voluntarism & Grantmaking | $160K | $635K |
| 97 | Wicksburg Youth Athletic Association | Recreation & Sports | $155K | $159K |
| 98 | Houston County Farmers Federation | Unclassified | $153K | $718K |
| 99 | Red Knot Development | International, Foreign Affairs & National Security | $146K | $215K |
| 100 | Rotary International | Unclassified | $139K | $108K |