| 1 | Southeastern Conference | Recreation & Sports | $2.9B | $351.0M |
| 2 | University of Alabama Health Services Foundation Pc | Health Care | $1.2B | $1.1B |
| 3 | Childrens Hospital of Alabama | Health Care | $953.2M | $988.2M |
| 4 | Navigate Affordable Housing Partners INC | Housing & Shelter | $919.3M | $93.2M |
| 5 | Samford University | Education | $400.2M | $905.5M |
| 6 | Haplotace Foundation | Philanthropy, Voluntarism & Grantmaking | $348.4M | $723.8M |
| 7 | The Community Foundation of Greater Birmingham | Philanthropy, Voluntarism & Grantmaking | $271.8M | $350.7M |
| 8 | St. Vincents Birmingham | Health Care | $182.8M | $305.7M |
| 9 | United Way of Central Alabama INC | Philanthropy, Voluntarism & Grantmaking | $133.9M | $141.4M |
| 10 | Southern Energy Credit Union | Unclassified | $119.3M | $3.5B |
| 11 | Baptist Health System INC | Health Care | $87.7M | $266.5M |
| 12 | St. Vincents East | Health Care | $84.8M | $125.2M |
| 13 | Alabama Childrens Hospital Foundation | Health Care | $77.0M | $1.6B |
| 14 | Uab Medicine Enterprise | Health Care | $64.4M | $259.4M |
| 15 | Lucille S Beeson Charitable Remainder 66 1055001761 | Philanthropy, Voluntarism & Grantmaking | $60.6M | $195.6M |
| 16 | Retail Wholesale & Department Store International Union & Industry | Unclassified | $60.0M | $68.8M |
| 17 | Southern Research Institute | Science & Technology | $53.8M | $238.7M |
| 18 | Community Food Bank of Central Alabama | Food, Agriculture & Nutrition | $53.6M | $32.3M |
| 19 | Regions Foundation | Philanthropy, Voluntarism & Grantmaking | $50.4M | $130.5M |
| 20 | Lakeshore Foundation | Health Care | $47.6M | $101.5M |
| 21 | Alabama Life & Disability Insurance Guaranty Assoc | Unclassified | $46.8M | $49.2M |
| 22 | St. Vincents Health System | Health Care | $43.9M | $77.4M |
| 23 | Michael and Noemi Neidorff Family Foundation | Philanthropy, Voluntarism & Grantmaking | $42.5M | $188.5M |
| 24 | Glenwood INC | Unclassified | $41.9M | $36.9M |
| 25 | Birmingham-southern College | Unclassified | $40.6M | $88.8M |
| 26 | Scholarships for Kids INC | Education | $31.9M | $45.0M |
| 27 | Young Mens Christian Assoc of Birmingham | Human Services | $31.8M | $64.0M |
| 28 | The Arc of Central Alabama INC | Unclassified | $31.8M | $12.7M |
| 29 | Alabama Power Foundation INC | Philanthropy, Voluntarism & Grantmaking | $29.2M | $117.9M |
| 30 | Southwestern Athletic Conference | Unclassified | $25.6M | $22.4M |
| 31 | Orlean and Ralph W Beeson Fund | Unclassified | $25.4M | $53.5M |
| 32 | Jefferson County Committee for Economic Opportunity | Human Services | $24.8M | $11.3M |
| 33 | Double Oaks Foundation | Philanthropy, Voluntarism & Grantmaking | $24.3M | $23.4M |
| 34 | Alabama Goodwill Industries INC | Unclassified | $24.0M | $35.2M |
| 35 | Altec-styslinger Foundation INC | Philanthropy, Voluntarism & Grantmaking | $23.8M | $142.9M |
| 36 | Christ Health Center INC | Health Care | $22.5M | $17.4M |
| 37 | Thompson Tractor Co INC Employee Benefit Trust | Unclassified | $21.7M | $5.7M |
| 38 | Zyne Family Foundation INC | Philanthropy, Voluntarism & Grantmaking | $20.5M | $24.2M |
| 39 | Presbyterian Retirement Homes of Birmingham INC | Unclassified | $19.1M | $49.7M |
| 40 | Mike and Gillian Goodrich Charitable Foundation | Philanthropy, Voluntarism & Grantmaking | $19.0M | $105.5M |
| 41 | Freedom Rain INC | Human Services | $19.0M | $9.9M |
| 42 | Aids Alabama INC | Diseases, Disorders & Medical Disciplines | $18.9M | $12.3M |
| 43 | Lifeline Childrens Services INC | Religion-Related | $18.7M | $8.1M |
| 44 | Alabama Business Charitable Trust Fund INC | Unclassified | $18.7M | $37.7M |
| 45 | I3 Academy | Education | $17.7M | $17.5M |
| 46 | Advancing Sight Network | Unclassified | $17.1M | $17.4M |
| 47 | Mountain Brook Club | Unclassified | $17.0M | $34.0M |
| 48 | Aletheia House INC | Mental Health & Crisis Intervention | $16.2M | $27.3M |
| 49 | Birmingham Promise INC | Education | $15.6M | $23.6M |
| 50 | United Ability INC | Diseases, Disorders & Medical Disciplines | $15.4M | $51.0M |
| 51 | Club Incorporated | Unclassified | $15.3M | $14.8M |
| 52 | University of Alabama Ophthalmology Services Foundation | Human Services | $15.0M | $1.7M |
| 53 | Birmingham Aids Outreach INC | Diseases, Disorders & Medical Disciplines | $14.9M | $3.3M |
| 54 | Holle Family Foundation | Philanthropy, Voluntarism & Grantmaking | $14.8M | $51.6M |
| 55 | Uab Educational Foundation | Education | $13.4M | $57.5M |
| 56 | Quarterbacking Childrens Health Foundation | Philanthropy, Voluntarism & Grantmaking | $12.6M | $35.4M |
| 57 | Alabama Central Credit Union | Unclassified | $12.5M | $200.7M |
| 58 | Max and Lorayne Cooper Foundation | Philanthropy, Voluntarism & Grantmaking | $12.5M | $25.5M |
| 59 | St. Vincents Blount | Health Care | $12.4M | $13.5M |
| 60 | One World Surgery | International, Foreign Affairs & National Security | $11.7M | $19.5M |
| 61 | Cornerstone Schools of Alabama INC | Unclassified | $11.4M | $18.0M |
| 62 | Cooper Family Foundation | Religion-Related | $11.2M | $13.2M |
| 63 | Greater Birmingham Humane Society | Animal-Related | $10.8M | $24.2M |
| 64 | Downtown Jimmie Hale Mission | Housing & Shelter | $10.6M | $17.8M |
| 65 | Legacy Schools | Education | $10.4M | $5.5M |
| 66 | New Schools for Alabama | Education | $10.3M | $8.2M |
| 67 | Alabama Insurance Guaranty Association | Unclassified | $10.0M | $160.2M |
| 68 | Baptist Health Foundation of Birmingham INC | Health Care | $9.8M | $41.7M |
| 69 | Scott Dawson Evangelistic Assoc INC | Unclassified | $9.7M | $14.6M |
| 70 | Alabama Educational Television Foundation Authority | Arts, Culture & Humanities | $9.2M | $15.1M |
| 71 | Childcare Resources | Human Services | $9.2M | $1.4M |
| 72 | Altamont School | Education | $9.1M | $25.1M |
| 73 | Eco Credit Union | Unclassified | $9.1M | $181.4M |
| 74 | Junior Achievement of Alabama INC | International, Foreign Affairs & National Security | $9.0M | $9.1M |
| 75 | Education Freedom Alabama | Education | $8.9M | $10.1M |
| 76 | Young Womens Christian Assn of Birmingham | Human Services | $8.9M | $8.9M |
| 77 | Robert R Meyer Foundation | Unclassified | $8.9M | $35.7M |
| 78 | Sturgis Charitable Tr | Philanthropy, Voluntarism & Grantmaking | $8.7M | $27.8M |
| 79 | Penny Foundation | Philanthropy, Voluntarism & Grantmaking | $8.6M | $829K |
| 80 | Collat Family Foundation INC | Unclassified | $8.6M | $13.7M |
| 81 | Propel Education | Employment | $8.5M | $26.4M |
| 82 | Womens Foundation of Alabama | Philanthropy, Voluntarism & Grantmaking | $8.5M | $11.7M |
| 83 | Womans Missionary Union Foundation | Human Services | $8.5M | $54.3M |
| 84 | Grand Slam Club North American Sheep Hunters Association | Animal-Related | $8.3M | $7.0M |
| 85 | Tractor & Equipment Health Care Trust | Unclassified | $8.2M | $6.4M |
| 86 | J Craig and Page T Smith Scholarship Foundation INC | Education | $8.1M | $23.0M |
| 87 | Hugh Kaul Foundation Tr 09-12-89 25-6733-00-7 | Unclassified | $8.0M | $50.4M |
| 88 | Ductile Iron Pipe Research Association | Unclassified | $7.9M | $1.7M |
| 89 | McWane Science Center | Arts, Culture & Humanities | $7.9M | $12.4M |
| 90 | The Economic Development Partnership of Alabama INC | Unclassified | $7.6M | $14.5M |
| 91 | Uab Research Foundation | Unclassified | $7.3M | $18.4M |
| 92 | Womans Missionary Union Auxiliary to Southern Baptist Convention | Religion-Related | $7.3M | $15.9M |
| 93 | Gateway | Human Services | $7.2M | $41.4M |
| 94 | Red Mountain Theatre Company INC | Arts, Culture & Humanities | $7.2M | $32.1M |
| 95 | Mountain Brook City Schools Foundation | Education | $7.1M | $18.7M |
| 96 | Community Partnership of Alabama INC | Education | $7.0M | $8.7M |
| 97 | Brocks Gap Training Center INC | Recreation & Sports | $6.9M | $7.4M |
| 98 | Make a Wish Foundation of Alabama INC | Health Care | $6.5M | $4.1M |
| 99 | Holy Name of Jesus Hospital Trust | Health Care | $6.2M | $26.6M |
| 100 | Linly Heflin Unit | Education | $6.2M | $10.2M |