| 1 | Wells Fargo & Company Employee Benefit Trust Des Moines | $2.7B | $2.7B | $14.1M |
| 2 | Prairie Meadows Race Track & Casino INC Altoona | $2.5B | $2.5B | $327.6M |
| 3 | Unitypoint at Home Wdm | $291.5M | $286.3M | $95.4M |
| 4 | Delta Dental of Iowa Johnston | $270.8M | $262.0M | $180.2M |
| 5 | Hy-vee Employee Benefit Plan Tr Wdm | $213.2M | $213.2M | $27.2M |
| 6 | Iowa Bankers Benefit Plan Johnston | $191.5M | $165.1M | $125.7M |
| 7 | Lincoln National Corporation Voluntary Employee Bene Assoc Tr Des Moines | $178.0M | $178.0M | $19.6M |
| 8 | Corn Belt Power Cooperative Humboldt | $171.6M | $171.6M | $570.1M |
| 9 | Iowa West Foundation Council Blfs | $152.2M | $0 | $495.7M |
| 10 | Iowa Farm Bureau Federation Wdm | $131.4M | $49.5M | $1.8B |
| 11 | Northwest Iowa Power Cooperative Le Mars | $114.6M | $114.6M | $279.0M |
| 12 | Impactlife Davenport | $91.5M | $91.4M | $55.3M |
| 13 | Loras College Dubuque | $85.6M | $63.3M | $112.3M |
| 14 | Goodwill Industries of the Heartland Cedar Rapids | $80.1M | $39.5M | $53.1M |
| 15 | Baylor College of Medicine Employer-contribution Veba Tr Clinton | $77.6M | $20.3M | $63.6M |
| 16 | The Principal Trust for Health Bens for Ees and Non-medicare Elig Rets Des Moines | $77.2M | $77.2M | $201.7M |
| 17 | Sioux Center Health Sioux Center | $77.2M | $77.0M | $112.0M |
| 18 | Cooperative Welfare Benefits Fund West des Moines | $76.7M | $65.3M | $20.6M |
| 19 | Linn County Rural Electric Cooperative Association Marion | $73.2M | $70.6M | $222.9M |
| 20 | Roy J Carver Charitable Tr Muscatine | $72.4M | $0 | $236.7M |
| 21 | Transamerica Foundation Cedar Rapids | $71.6M | $0 | $172.1M |
| 22 | Grhs Holdings INC W Burlington | $69.6M | $8.0M | $260.7M |
| 23 | Eastern Iowa Light and Power Cooperative Wilton | $68.8M | $68.5M | $211.6M |
| 24 | Imagine the Possibilities INC Maquoketa | $67.6M | $67.0M | $62.4M |
| 25 | City of Raleigh North Carolina Medical Benefits Trust Des Moines | $66.5M | $66.5M | $33.7M |
| 26 | North West Rural Electric Cooperative Orange City | $61.8M | $61.5M | $153.4M |
| 27 | National Pork Producers Council Des Moines | $60.9M | $26.2M | $39.1M |
| 28 | Midland Power Cooperative Boone | $60.6M | $60.1M | $169.3M |
| 29 | Dubuque Racing Association Ltd Dubuque | $59.4M | $35.3M | $93.8M |
| 30 | Iowa State Association of Counties Wdm | $57.9M | $47.4M | $51.0M |
| 31 | Medical Associates Clinic Health Plan of Wisconsin Dubuque | $56.7M | $56.0M | $7.9M |
| 32 | Hope Haven Incorporated Rock Valley | $56.7M | $56.3M | $49.0M |
| 33 | Community Health Care INC Davenport | $54.9M | $54.5M | $40.8M |
| 34 | Iowa Soybean Association Ankeny | $53.8M | $53.8M | $54.5M |
| 35 | Iowa PBS Foundation Johnston | $52.3M | $15.5M | $42.6M |
| 36 | Maquoketa Valley Rural Electric Cooperative Anamosa | $45.6M | $45.6M | $169.7M |
| 37 | Iowa Donor Network North Liberty | $45.4M | $38.6M | $37.5M |
| 38 | Richard O Jacobson Foundation INC Des Moines | $45.0M | $0 | $66.7M |
| 39 | Corda Credit Union Cedar Rapids | $44.2M | $44.2M | $686.6M |
| 40 | South Slope Cooperative Telephone Company INC North Liberty | $43.5M | $43.5M | $113.3M |
| 41 | Impact Community Action Partnership INC Des Moines | $43.1M | $43.1M | $2.5M |
| 42 | L & O Power Cooperative Rock Rapids | $43.1M | $43.1M | $80.5M |
| 43 | Access Energy Cooperative Mt Pleasant | $41.4M | $41.4M | $111.2M |
| 44 | Central Iowa Juvenile Detention Center Eldora | $40.8M | $40.8M | $13.0M |
| 45 | Plumbers & Steamfitters H & W Trust Iowa State Bank Ttee Des Moines | $37.9M | $32.5M | $98.0M |
| 46 | Iowa Laborers District Council Health and Welfare Plan Cedar Rapids | $36.3M | $30.4M | $22.1M |
| 47 | Butler County Rural Electric Cooperative Allison | $35.0M | $34.8M | $92.1M |
| 48 | Catholic Foundation for the Diocese of Davenport Davenport | $34.4M | $2.1M | $30.3M |
| 49 | United Way of Central Iowa Des Moines | $33.9M | $25.7M | $38.6M |
| 50 | Heartland Power Cooperative Thompson | $33.8M | $33.7M | $69.7M |