| 1 | Aerospace Corporation Chantilly | $1.4B | $1.4B | $1.3B |
| 2 | Noblis INC Reston | $673.8M | $614.5M | $452.3M |
| 3 | Institute for Defense Analyses Alexandria | $360.1M | $350.3M | $607.9M |
| 4 | Bionexus Foundation INC Manassas | $322.0M | $9.1M | $323.5M |
| 5 | American Type Culture Collection Manassas | $205.3M | $198.0M | $260.2M |
| 6 | Analytic Services INC Arlington | $174.0M | $138.6M | $265.6M |
| 7 | Associated Universities INC Vienna | $152.5M | $143.5M | $71.3M |
| 8 | US Civilian Research and Development Foundation Arlington | $140.0M | $73.6M | $79.8M |
| 9 | Public Interest Registry Reston | $137.1M | $106.4M | $71.4M |
| 10 | Logistics Management Institute Tysons | $127.6M | $30.3M | $447.9M |
| 11 | American Society of Civil Engineers Reston | $53.1M | $53.0M | $85.9M |
| 12 | Virginia Innovation Partnership Corporation Herndon | $47.4M | $47.4M | $80.0M |
| 13 | Academy of Managed Care Pharmacy a New Jersey Nonprofit Corporation Alexandria | $30.7M | $20.9M | $19.9M |
| 14 | Commonwealth Center for Advanced Manufacturing Disputanta | $24.2M | $23.3M | $13.7M |
| 15 | National Society of Black Engineers Alexandria | $22.7M | $22.7M | $31.9M |
| 16 | Virginia Tech Applied Research Corporation Arlington | $21.1M | $21.1M | $13.6M |
| 17 | Connected Giving Foundation Fredericksburg | $16.6M | $5.6M | $69.7M |
| 18 | The National Council of Teachers of Mathematics-incorporated- Reston | $15.0M | $11.2M | $16.6M |
| 19 | Geoscienceworld McLean | $13.6M | $10.0M | $8.8M |
| 20 | American Association of Physicists in Medicine Alexandria | $12.4M | $12.0M | $30.3M |
| 21 | Concrete Advancement Foundation INC Alexandria | $12.1M | $3.0M | $26.4M |
| 22 | Virginia Health Information Richmond | $11.7M | $11.6M | $18.6M |
| 23 | Amazon Conservation Team Falls Church | $11.5M | $5.7M | $18.3M |
| 24 | Institute of Navigation Manassas | $11.3M | $7.2M | $25.6M |
| 25 | National Institute of Aerospace Associates Hampton | $10.8M | $10.8M | $15.3M |
| 26 | Analysis and Resilience Center for Systemic Risk INC Arlington | $10.7M | $10.7M | $12.3M |
| 27 | Center for Open Science Charlottesvle | $10.2M | $10.2M | $16.3M |
| 28 | Ocean Visions INC Leesburg | $8.3M | $8.3M | $9.9M |
| 29 | Zcash Foundation McLean | $8.2M | $3.8M | $23.4M |
| 30 | Building Change INC Richmond | $4.7M | $4.7M | $825K |
| 31 | Center for Excellence in Education McLean | $4.2M | $4.2M | $11.9M |
| 32 | Corporation for National Research Initiatives Reston | $4.1M | $3.1M | $7.2M |
| 33 | Korean-american Scientists and Engineers Association INC Vienna | $4.0M | $3.9M | $11.9M |
| 34 | Korea-us Science Cooperation Center Vienna | $3.2M | $0 | $4.2M |
| 35 | American Institute of Biological Sciences INC Herndon | $3.2M | $2.9M | $2.6M |
| 36 | International Society for Computational Biology INC Leesburg | $2.5M | $2.4M | $3.5M |
| 37 | National Security Space Association INC Arlington | $2.5M | $2.5M | $854K |
| 38 | American Society of Naval Engineers Alexandria | $2.3M | $2.3M | $2.0M |
| 39 | Mineralogical Society of America Chantilly | $2.3M | $1.7M | $5.8M |
| 40 | Global Cold Chain Foundation Arlington | $2.3M | $2.3M | $1.4M |
| 41 | Bgh Foundation Fredericksbrg | $2.2M | $2.2M | $2.0M |
| 42 | Geochemical Society Alexandria | $1.9M | $1.9M | $4.0M |
| 43 | Association of Technology Leaders in Independent Schools Winchester | $1.8M | $1.8M | $657K |
| 44 | Information Technology Information Sharing Analysis Center INC Manassas | $1.7M | $1.7M | $2.7M |
| 45 | Society for Industrial Microbiology and Biotechnology Fairfax | $1.4M | $1.4M | $1.6M |
| 46 | Greenwater Foundation Arlington | $1.2M | $1.2M | $679K |
| 47 | Fed Tech Cares Great Falls | $1.1M | $1.1M | $1.1M |
| 48 | National Society of Black Physicists Arlington | $1.1M | $1.1M | $746K |
| 49 | American Astronautical Society INC Springfield | $1.1M | $1.0M | $1.2M |
| 50 | Society for Imaging Science and Technology Springfield | $1.0M | $1.0M | $700K |