| 1 | Howard Hughes Medical Institute Chevy Chase | $20.1B | $2.0B | $29.5B |
| 2 | Henry M Jackson Foundation for the Advancement of Military Medicine Bethesda | $631.6M | $628.9M | $321.6M |
| 3 | Foundation for the National Institute of Health INC Rockville | $432.6M | $78.5M | $214.1M |
| 4 | American College of Gastroenterology INC Rockville | $146.5M | $29.0M | $316.9M |
| 5 | The Gog Foundation INC Edgewater | $86.2M | $68.5M | $145.5M |
| 6 | Foundation Fighting Blindness INC Columbia | $70.8M | $36.6M | $110.9M |
| 7 | Lieber Institute INC Baltimore | $58.7M | $31.3M | $35.1M |
| 8 | American Urological Association Education and Research INC Linthicum | $36.5M | $35.9M | $227.0M |
| 9 | Cvpath Institute INC Gaithersburg | $22.7M | $13.1M | $23.5M |
| 10 | Immune Deficiency Foundation Hanover | $20.9M | $10.1M | $18.8M |
| 11 | Aoac International Rockville | $11.6M | $6.6M | $9.1M |
| 12 | Cancer Research America Nfcr INC Rockville | $11.2M | $11.1M | $12.3M |
| 13 | The Arvo Foundation for Eye Research INC Gaithersburg | $10.2M | $1.6M | $9.0M |
| 14 | Foundation Fighting Blindness Retinal Degeneration Fund Columbia | $9.0M | $7.0M | $59.6M |
| 15 | The Stanley Medical Research Institute Rockville | $8.3M | $6.3M | $93.3M |
| 16 | Anatomic Gift Foundation INC Hanover | $8.3M | $8.3M | $4.4M |
| 17 | Baltimore Research & Education Foundation INC Baltimore | $7.7M | $7.7M | $26.1M |
| 18 | Bladder Cancer Advocacy Network INC Bethesda | $6.4M | $6.4M | $15.8M |
| 19 | Caring Cross INC Gaithersburg | $6.4M | $6.4M | $16.5M |
| 20 | Urology Care Foundation INC Linthicum Hts | $5.5M | $5.4M | $79.9M |
| 21 | Biohealth Innovation INC Rockville | $4.4M | $4.4M | $5.3M |
| 22 | Blackbird Laboratories INC Baltimore | $4.3M | $4.3M | $85.5M |
| 23 | Heart Failure Society of America INC Rockville | $4.2M | $4.2M | $10.7M |
| 24 | American Speech-language-hearing Foundation Rockville | $4.0M | $2.1M | $23.9M |
| 25 | American Society for Investigative Pathology INC Rockville | $3.8M | $2.1M | $3.3M |
| 26 | Center for Disease Dynamics Economics & Policy INC Silver Spring | $3.7M | $3.7M | $15.1M |
| 27 | Diabetes Research and Wellness Foundation INC Bethesda | $3.2M | $3.2M | $828K |
| 28 | Osteogenesis Imperfecta Foundation INC Gaithersburg | $2.7M | $2.3M | $2.1M |
| 29 | The American Porphyrias Expert Collaborative INC Bethesda | $2.3M | $2.3M | $2.2M |
| 30 | Alcoholic Beverage Medical Research Foundation Bel Air | $2.3M | $0 | $3.2M |
| 31 | Norton M Luger and Rosalind Massow Luger Charitable Tr I Bethesda | $2.2M | $0 | $1.7M |
| 32 | National Pancreas Foundation Bethesda | $1.7M | $1.4M | $499K |
| 33 | Tissue Bank Asbestos Research Charitable Trust Lanham | $1.7M | $350K | $10.0M |
| 34 | Hope for Henry Foundation Glen Echo | $1.6M | $1.6M | $1.0M |
| 35 | Aim-hi Accelerator Fund INC Rockville | $1.6M | $1.6M | $16.1M |
| 36 | The Lilabean Foundation INC Silver Spring | $1.5M | $1.2M | $874K |
| 37 | Brem Foundation to Defeat Breast Cancer INC Silver Spring | $1.5M | $1.4M | $686K |
| 38 | American Association on Health and Disability INC Rockville | $1.2M | $1.2M | $1.7M |
| 39 | United States Association for the Study of Pain INC Annapolis | $1.1M | $1.1M | $1.2M |
| 40 | Medical Technology and Practice Patterns Institute INC Bethesda | $911K | $911K | $4.3M |
| 41 | Susan & Teresa Schwab Ovarian Cancer Research Foundation INC Annapolis | $899K | $0 | $1.7M |
| 42 | The Focus Foundation INC Crofton | $833K | $795K | $506K |
| 43 | Image Guided Therapy Research Institute Rockville | $735K | $735K | $3K |
| 44 | Hurwitz Breast Cancer Fund INC Frederick | $720K | $660K | $1.9M |
| 45 | Foundation for Rare Disease Research INC Silver Spring | $669K | $669K | $2.1M |
| 46 | Camellus and Sirlyne Ezeugwu Foundation Trust Ellicott City | $610K | $0 | $657K |
| 47 | Laughlin Family Foundation for Rare Cancers Crofton | $538K | $364K | $195K |
| 48 | Prevent Alzheimers Disease 2020 Potomac | $442K | $442K | $18K |
| 49 | Determined2heal Foundation INC Potomac | $409K | $409K | $810K |
| 50 | There Goes My Hero Bel Air | $295K | $295K | $266K |