| 1 | Iso New England INC Holyoke | $264.6M | $264.6M | $700.2M |
| 2 | Massachusetts Audubon Society INC Lincoln | $80.5M | $73.7M | $380.9M |
| 3 | Trustees of Reservations Boston | $62.5M | $57.9M | $398.2M |
| 4 | Appalachian Mountain Club Charlestown | $55.0M | $43.6M | $221.0M |
| 5 | Clean Air Task Force INC Boston | $50.8M | $50.1M | $47.2M |
| 6 | Ceres INC Boston | $47.3M | $40.9M | $39.0M |
| 7 | Conservation Law Foundation INC Boston | $42.4M | $41.6M | $54.1M |
| 8 | New England Interstate Water Pollution Control Commission Lowell | $26.1M | $26.1M | $15.2M |
| 9 | Nantucket Conservation Foundation INC Nantucket | $24.1M | $17.5M | $178.4M |
| 10 | Project Innerspace INC Boston | $23.1M | $23.1M | $17.8M |
| 11 | Buzzards Bay Coalition INC New Bedford | $22.6M | $15.7M | $51.9M |
| 12 | Sconset Trust INC Siasconset | $21.4M | $16.3M | $59.1M |
| 13 | Worcester County Horticultural Society Boylston | $21.0M | $8.7M | $59.4M |
| 14 | Massachusetts Service Alliance INC Boston | $18.9M | $18.9M | $6.1M |
| 15 | Deploy-us INC Boston | $16.2M | $16.2M | $5.3M |
| 16 | Greentown Collaborative INC Somerville | $15.0M | $15.0M | $37.4M |
| 17 | Cathleen Stone Island Outward Bound School INC Boston | $14.9M | $14.7M | $16.4M |
| 18 | Goodpower Education Fund INC Boston | $14.3M | $14.3M | $5.5M |
| 19 | Polly Hill Arboretum INC West Tisbury | $13.1M | $1.7M | $34.2M |
| 20 | Manomet INC Manomet | $13.0M | $8.3M | $34.1M |
| 21 | Center for Ecotechnology INC Northampton | $12.0M | $12.0M | $6.1M |
| 22 | Berkshire Natural Resources Lenox | $11.4M | $6.7M | $37.2M |
| 23 | Orleans Conservation Trust Orleans | $10.5M | $8.9M | $51.2M |
| 24 | Boston Harbor Now INC Boston | $10.5M | $10.1M | $9.7M |
| 25 | Center for Coastal Studies INC Provincetown | $10.4M | $10.2M | $10.9M |
| 26 | New England Forestry Foundation INC Littleton | $10.3M | $9.1M | $50.6M |
| 27 | Essex County Greenbelt Association Essex | $9.9M | $9.0M | $72.4M |
| 28 | Native Plant Trust INC Wayland | $9.0M | $4.8M | $23.2M |
| 29 | Friends of the Public Garden INC Boston | $9.0M | $5.7M | $31.5M |
| 30 | New Ecology INC Boston | $8.4M | $8.4M | $5.7M |
| 31 | Infact Boston | $8.0M | $8.0M | $4.9M |
| 32 | Efficiency Forward INC Medford | $7.8M | $7.8M | $5.3M |
| 33 | Sheriffs Meadow Foundation West Tisbury | $7.5M | $4.5M | $100.3M |
| 34 | Mystic River Watershed Association INC Arlington | $6.5M | $6.5M | $10.8M |
| 35 | Native Land Conservancy INC Mashpee | $6.5M | $6.5M | $18.0M |
| 36 | Harwich Conservation Tr South Harwich | $6.1M | $6.1M | $24.7M |
| 37 | Environmental League of Massachusetts INC Boston | $6.0M | $5.6M | $6.5M |
| 38 | Duxbury Beach Reservation INC Duxbury | $5.9M | $4.1M | $6.8M |
| 39 | Northeast Energy Efficiency Partnerships INC Woburn | $5.9M | $5.9M | $5.2M |
| 40 | Sudbury Valley Trustees INC Sudbury | $5.9M | $5.8M | $38.2M |
| 41 | Nantucket Land and Water Council INC Nantucket | $5.7M | $1.9M | $42.7M |
| 42 | Cascade Climate INC Somerville | $5.7M | $5.7M | $5.7M |
| 43 | Furniture Trust Organization INC Arlington | $5.6M | $5.3M | $982K |
| 44 | Friends of the Arava Institute Ltd Newton Center | $5.4M | $4.4M | $6.5M |
| 45 | Connecticut River Watershed Council INC Greenfield | $5.1M | $3.7M | $5.6M |
| 46 | Populace INC Cambridge | $5.1M | $5.1M | $12.1M |
| 47 | Northeast States for Coordinated Air Use Management INC Boston | $5.1M | $5.0M | $13.5M |
| 48 | Kestrel Land Trust INC Amherst | $4.7M | $2.6M | $10.4M |
| 49 | Homeworld Collective INC Somerville | $4.7M | $4.7M | $4.6M |
| 50 | Greenroots INC Chelsea | $4.3M | $4.3M | $12.6M |