| 1 | Iowa Natural Heritage Foundation Des Moines | $37.6M | $29.8M | $160.4M |
| 2 | Greater des Moines Botanical Garden Des Moines | $12.8M | $11.8M | $31.4M |
| 3 | Iowa Confluence Water Trails Des Moines | $10.5M | $10.4M | $52.1M |
| 4 | The Spirit of Okoboji Okoboji | $8.4M | $8.4M | $9.1M |
| 5 | Conservation Foundation of Dickinson County Okoboji | $6.5M | $6.4M | $1.6M |
| 6 | Water Professionals International Urbandale | $4.1M | $3.7M | $4.1M |
| 7 | Iowa Agriculture Water Alliance Ankeny | $3.9M | $3.9M | $2.7M |
| 8 | Trees Forever INC Marion | $3.5M | $3.5M | $4.1M |
| 9 | Indian Creek Nature Center Cedar Rapids | $3.2M | $3.1M | $11.4M |
| 10 | Deb & Jeff Hansen Foundation Wdm | $2.6M | $1.9M | $7.3M |
| 11 | Iowa Environmental Council Des Moines | $2.1M | $1.9M | $2.1M |
| 12 | Pathfinders Resource Conservation and Development Area INC Fairfield | $2.1M | $2.1M | $2.1M |
| 13 | Nahant Marsh Education Center Davenport | $1.8M | $1.8M | $5.0M |
| 14 | Iowa Valley Resource Conservation & Development Amana | $1.8M | $1.8M | $725K |
| 15 | Soil and Water Conservation Society Ankeny | $1.7M | $1.2M | $2.0M |
| 16 | Iowa Agricultural Mitigation Boone | $1.4M | $1.4M | $1.7M |
| 17 | Whiterock Conservancy Coon Rapids | $1.4M | $1.4M | $24.6M |
| 18 | Dubuque Arboretum Association INC Dubuque | $1.2M | $886K | $3.2M |
| 19 | Iowa Nutrient Research and Education Council Des Moines | $1.1M | $1.1M | $442K |
| 20 | Iowa Arboretum INC Madrid | $1.1M | $1.1M | $6.4M |
| 21 | Resource Conservation and Development for Northeast Iowa INC Postville | $1.0M | $1.0M | $2.3M |
| 22 | Mason City Foundation Mason City | $945K | $909K | $5.7M |
| 23 | Agricultures Clean Water Alliance Ankeny | $857K | $857K | $369K |
| 24 | Green Schools National Network INC Iowa City | $791K | $791K | $151K |
| 25 | Golden Hills Resource Conservation and Development Area Oakland | $784K | $784K | $1.0M |
| 26 | Keep Iowa Beautiful Wdm | $745K | $733K | $525K |
| 27 | Iowa Great Lakes Beautification Okoboji | $701K | $696K | $5.1M |
| 28 | William G Stowe Memorial Foundation Des Moines | $618K | $618K | $810K |
| 29 | Sustainable Iowa Land Trust West Branch | $539K | $539K | $3.8M |
| 30 | Iowa Business for Clean Energy Wdm | $527K | $527K | $419K |
| 31 | Clinton County Conservation Foundation INC Grand Mound | $514K | $298K | $552K |
| 32 | Clean Energy Districts of Iowa Decorah | $465K | $465K | $306K |
| 33 | Izaak Walton League of America INC Newton | $441K | $238K | $232K |
| 34 | Izaak Wlaton League of America INC Des Moines | $425K | $106K | $737K |
| 35 | Iowa Drainage District Assoc Wdm | $370K | $370K | $137K |
| 36 | Southwest Iowa Nature Trails Project INC Imogene | $361K | $352K | $844K |
| 37 | Des Moines Chapter of the Izaak Walton League of America Des Moines | $333K | $212K | $335K |
| 38 | Iowa Storm Water Education Partnership Des Moines | $325K | $325K | $220K |
| 39 | Cedar Valley Arboretum & Botanic Gardens Waterloo | $309K | $300K | $812K |
| 40 | Holiday Lights at the Lake Coralville | $304K | $185K | $140K |
| 41 | Agricultural Drainage Management Coalition INC Ankeny | $299K | $299K | $29K |
| 42 | Soil Enhancement and Subsurface Water Management Coalition Dexter | $296K | $296K | $397 |
| 43 | Rabbit Hollow Nature Conservancy INC Dubuque | $295K | $0 | $1.5M |
| 44 | Iowa Great Lakes Foundation Des Moines | $280K | $280K | $749K |
| 45 | Izaak Walton League of America INC Davenport | $271K | $98K | $86K |
| 46 | Iowa Water Environment Association Ames | $266K | $266K | $395K |
| 47 | A M O S-a Metropolitan Organizing Strategy Des Moines | $258K | $257K | $403K |
| 48 | Iowa Interfaith Power and Light Des Moines | $247K | $247K | $128K |
| 49 | Energy Association of Iowa Schools Creston | $238K | $238K | $252K |
| 50 | N-compass INC Marion | $233K | $233K | $180K |