| 1 | Mds Famly Foundation INC Maitland | $45.3M | $0 | $66.0M |
| 2 | The Everglades Foundation INC Palmetto Bay | $31.1M | $17.7M | $54.9M |
| 3 | Marie Selby Botanical Gardens INC Sarasota | $29.2M | $17.6M | $110.8M |
| 4 | Environment & Coral Reef Conservation Foundation INC Hialeah | $28.7M | $0 | $16.3M |
| 5 | Naples Botanical Garden INC Naples | $28.6M | $27.4M | $74.0M |
| 6 | Edward E Hadddock Jr Family Operating Foundation Orlando | $23.3M | $0 | $38.3M |
| 7 | Tall Timbers Foundation INC Tallahassee | $19.1M | $3.9M | $44.1M |
| 8 | Gasparilla Island Conservation and Improvement Association INC Boca Grande | $17.5M | $3.0M | $54.5M |
| 9 | Fairchild Tropical Botanic Garden Miami | $14.0M | $13.3M | $51.5M |
| 10 | Sanibel-captiva Conservation Foundation INC Sanibel | $14.0M | $13.6M | $56.9M |
| 11 | North Florida Land Trust INC Jacksonville | $12.6M | $11.2M | $28.7M |
| 12 | Alachua Conservation Trust Incorporated Gainesville | $9.4M | $9.4M | $39.4M |
| 13 | Nokuse Education INC Freeport | $8.9M | $8.9M | $28.2M |
| 14 | Coral Restoration Foundation INC Tavernier | $8.9M | $8.8M | $13.3M |
| 15 | U S Water Alliance Tampa | $8.5M | $8.5M | $7.1M |
| 16 | Big Waters Land Trust INC Osprey | $8.1M | $4.9M | $38.9M |
| 17 | Conservation Florida INC Orlando | $7.6M | $3.6M | $18.9M |
| 18 | Childrens Case Management Organization INC West Palm Bch | $7.0M | $7.0M | $3.8M |
| 19 | Jewish Climate Trust INC Palm Beach | $6.7M | $6.7M | $6.2M |
| 20 | Groundwork Jacksonville INC Jacksonville | $6.6M | $6.6M | $3.4M |
| 21 | Florida Audubon Society Coral Gables | $5.5M | $1.5M | $12.5M |
| 22 | Tampa Bay History Center INC Tampa | $5.1M | $4.9M | $20.9M |
| 23 | Save Crystal River INC Crystal River | $5.0M | $5.0M | $1.2M |
| 24 | Kenneth Kirchman Foundation INC Saint Cloud | $4.7M | $0 | $169.4M |
| 25 | McKee Botanical Garden INC Vero Beach | $4.6M | $4.1M | $24.0M |
| 26 | Tampa Baywatch INC St. Petersburg | $4.4M | $4.3M | $7.4M |
| 27 | Biscayne Bay Waterkeeper INC Coral Gables | $4.3M | $4.3M | $3.9M |
| 28 | African Community and Conservation Foundation INC Palm Beach | $4.2M | $4.2M | $856K |
| 29 | Solar and Energy Loan Fund of St. Lucie County INC Fort Pierce | $4.2M | $4.2M | $25.7M |
| 30 | Natureconnect Florida INC Atlantic Bch | $4.0M | $0 | $2.0M |
| 31 | Homosassa River Restoration Project INC Homosassa | $3.9M | $3.9M | $3.0M |
| 32 | Guy Harvey Ocean Fund INC Fort Lauderdale | $3.7M | $3.6M | $2.5M |
| 33 | The Funders Network INC Coral Gables | $3.7M | $3.7M | $4.3M |
| 34 | Florida Wildlife Corridor Foundation INC St. Petersburg | $3.7M | $3.7M | $8.4M |
| 35 | Underline Management Organization INC Miami | $3.6M | $3.6M | $896K |
| 36 | Winnie Palmer Nature Reserve Orlando | $3.5M | $260K | $7.8M |
| 37 | Live Wildly Foundation INC Tampa | $3.4M | $0 | $473K |
| 38 | Indian River Land Tr INC Vero Beach | $3.3M | $3.2M | $38.2M |
| 39 | Captains for Clean Water INC Fort Myers | $3.2M | $2.5M | $4.0M |
| 40 | Secore International INC Miami | $3.2M | $3.2M | $3.1M |
| 41 | Partnership for Gulf Coast Land Conservation INC Tallahassee | $3.0M | $3.0M | $2.0M |
| 42 | Israel Nature and Heritage Foundation of America INC Pembroke Pnes | $3.0M | $3.0M | $2.7M |
| 43 | Ocean Research & Conservation Association INC Vero Beach | $2.7M | $2.7M | $4.2M |
| 44 | Ecosphere Restoration Institute INC Largo | $2.7M | $2.7M | $987K |
| 45 | Earth Inbalance Corporation Orlando | $2.7M | $2.7M | $2.4M |
| 46 | Florida Three Rivers Resource Conservation and Development Milton | $2.7M | $0 | $855K |
| 47 | Cleo Institute INC Miami | $2.6M | $2.6M | $757K |
| 48 | Jacksonville Arboretum & Gardens INC Jacksonville | $2.6M | $2.6M | $2.7M |
| 49 | Climate Summit Miami Beach Host Committee INC Miami | $2.5M | $2.5M | $331K |
| 50 | Pigeon Key Foundation Marathon | $2.5M | $2.2M | $3.6M |