| 1 | American Society of Clinical Oncology Alexandria | $177.2M | $159.1M | $321.7M |
| 2 | American Diabetes Association INC Arlington | $165.8M | $152.2M | $253.9M |
| 3 | American College of Radiology Reston | $147.2M | $136.8M | $289.1M |
| 4 | March of Dimes INC Arlington | $103.2M | $83.6M | $64.2M |
| 5 | Amyotrophic Lateral Sclerosis Assn. Arlington | $102.4M | $68.6M | $107.2M |
| 6 | Accessia Health Midlothian | $45.3M | $37.6M | $119.1M |
| 7 | Public Health Accreditation Board Alexandria | $26.9M | $24.5M | $11.0M |
| 8 | American Association for the Study of Liver Diseases Alexandria | $20.8M | $15.9M | $34.2M |
| 9 | American Tinnitus Association Vienna | $17.7M | $4.5M | $10.6M |
| 10 | Society of Nuclear Medicine and Molecular Imaging Reston | $16.7M | $16.7M | $24.3M |
| 11 | Focused Ultrasound Foundation Charlottesvle | $16.6M | $16.6M | $39.1M |
| 12 | National Psoriasis Foundation Alexandria | $14.3M | $12.6M | $14.9M |
| 13 | Townebank Foundation Suffolk | $14.1M | $0 | $20.0M |
| 14 | Lgbt Life Center Norfolk | $11.9M | $11.9M | $6.8M |
| 15 | American Roentgen Ray Society Leesburg | $11.9M | $10.9M | $36.6M |
| 16 | American Society of Transplant Surgeons Arlington | $11.8M | $11.8M | $6.4M |
| 17 | Amer Acad of Otolarnygology Head & Neck Surgery Foundation INC Alexandria | $11.3M | $11.3M | $26.7M |
| 18 | American Society of Tropical Medicine and Hygiene Arlington | $7.9M | $7.4M | $15.1M |
| 19 | Society of General Internal Medicine Alexandria | $6.3M | $6.0M | $9.3M |
| 20 | United Cerebral Palsy INC Vienna | $5.9M | $2.1M | $11.6M |
| 21 | Minority Aids Support Services INC Newport News | $5.5M | $5.5M | $3.2M |
| 22 | Sjogrens Foundation INC Reston | $5.4M | $5.3M | $5.2M |
| 23 | Society for Imaging Informatics in Medicine-siim Leesburg | $5.1M | $2.6M | $8.8M |
| 24 | Conexus N Chesterfld | $4.9M | $4.8M | $4.4M |
| 25 | National Certification Commission for Acupuncture & Oriental Alexandria | $4.8M | $4.5M | $5.2M |
| 26 | Roc Solid Foundation INC Chesapeake | $4.8M | $4.8M | $2.4M |
| 27 | Bone Health and Osteoporosis Foundation Arlington | $4.6M | $4.6M | $7.0M |
| 28 | American Society of Nuclear Cardiology Fairfax | $4.6M | $4.6M | $9.5M |
| 29 | Insight Memory Care Center Fairfax | $4.1M | $4.0M | $4.0M |
| 30 | Brain Injury Services INC Springfield | $3.9M | $3.9M | $3.0M |
| 31 | Autism Program of Virginia INC Tap-va INC Richmond | $3.9M | $3.9M | $8.2M |
| 32 | Retired Professional Football Players Common Good Fund INC Arlington | $3.8M | $3.6M | $9.1M |
| 33 | Registry of Interpreters for the Deaf Arlington | $3.8M | $3.7M | $5.0M |
| 34 | American Congress of Rehabilitation Medicine Reston | $3.6M | $3.6M | $2.7M |
| 35 | Academic Pediatric Association McLean | $3.4M | $3.2M | $2.0M |
| 36 | Brain Injury Services of Swva Roanoke | $3.3M | $2.1M | $2.5M |
| 37 | Investigative Research Center INC Arlington | $3.2M | $3.2M | $138K |
| 38 | American Council of the Blind INC Alexandria | $3.2M | $3.0M | $6.3M |
| 39 | American Osteopathic Academy of Orthopedics Richmond | $3.1M | $2.4M | $5.8M |
| 40 | Fredericksburg Area Health and Support Services Incorporated Fredericksburg | $3.1M | $3.1M | $1.6M |
| 41 | Sarnoff Cardiovascular Research Foundation INC McLean | $2.9M | $2.6M | $50.9M |
| 42 | Dialysis Patient Citizens INC Arlington | $2.9M | $2.9M | $1.6M |
| 43 | Association of Pediatric Hematology Oncology Nurses INC McLean | $2.7M | $2.6M | $1.7M |
| 44 | Lions Medical Eye Bank and Research Center of Eastern Virginia INC Norfolk | $2.7M | $2.7M | $3.1M |
| 45 | Novasalud Falls Church | $2.6M | $2.6M | $2.3M |
| 46 | Special Love INC Winchester | $2.6M | $1.9M | $5.2M |
| 47 | Epilepsy Study Consortium INC Herndon | $2.5M | $2.5M | $3.7M |
| 48 | Cure Jm Foundation Leesburg | $2.3M | $2.3M | $2.6M |
| 49 | The Bridge Line Charlottesvle | $2.3M | $1.9M | $4.0M |
| 50 | Spina Bifida Association of America Alexandria | $2.1M | $1.9M | $714K |