| 1 | Honorbridge Greenville | $67.5M | $60.1M | $74.0M |
| 2 | American Board of Pediatrics INC Chapel Hill | $56.6M | $52.8M | $232.8M |
| 3 | The American Board of Anesthesiology INC Raleigh | $43.7M | $26.6M | $87.6M |
| 4 | Dont Ever Give Up INC Cary | $34.6M | $24.6M | $19.3M |
| 5 | Gha Autism Supports Albemarle | $17.1M | $17.1M | $11.8M |
| 6 | Bluewest Opportunities INC Asheville | $11.8M | $11.8M | $5.9M |
| 7 | Harborpath INC Charlotte | $10.9M | $10.9M | $2.5M |
| 8 | Miraclefeet Chapel Hill | $9.0M | $9.0M | $18.2M |
| 9 | Aces for Autism Greenville | $8.8M | $8.7M | $22.6M |
| 10 | No Baby Blisters Winston Salem | $7.4M | $7.4M | $6.3M |
| 11 | Carolinas Care Partnership Charlotte | $6.6M | $6.6M | $3.8M |
| 12 | Autism Services of Mecklenburg County Charlotte | $5.5M | $5.5M | $3.9M |
| 13 | Charlotte Neuroscience Association INC Charlotte | $5.4M | $5.4M | $4.2M |
| 14 | Jaime Leandro Foundation for Therapeutic Cancer Vaccines Chapel Hill | $4.8M | $4.8M | $3.0M |
| 15 | Metrolina Association for the Blind INC Charlotte | $4.6M | $4.6M | $2.1M |
| 16 | Association of Women in Rheumatology Fayetteville | $4.1M | $4.1M | $5.4M |
| 17 | Living with Autism INC Raleigh | $3.9M | $3.9M | $497K |
| 18 | Southeast Clinical Oncology Research Consortium INC Winston Salem | $2.9M | $2.9M | $1.5M |
| 19 | Autism Foundation of North Carolina INC Raleigh | $2.6M | $2.6M | $6.4M |
| 20 | 24 Foundation INC Charlotte | $2.5M | $2.5M | $1.9M |
| 21 | Cancer Services INC Winston Salem | $2.4M | $1.3M | $4.4M |
| 22 | Destination Hope Nc Winston Salem | $2.1M | $1.5M | $1.3M |
| 23 | Aids Leadership Foothills Area Alliance INC Hickory | $1.9M | $1.9M | $1.4M |
| 24 | Shallow Ford Foundation INC Clemmons | $1.9M | $1.9M | $18.2M |
| 25 | Lung Cancer Initiative Raleigh | $1.8M | $1.3M | $1.3M |
| 26 | Beginnings for Parents of Children Who Are Deaf or Hard of Hearing Raleigh | $1.8M | $1.8M | $530K |
| 27 | Oasis Nc Wilmington | $1.6M | $1.1M | $365K |
| 28 | Stxbp1 Foundation Holly Springs | $1.4M | $1.4M | $1.5M |
| 29 | North Carolina Dental Society Foundation INC Cary | $1.4M | $1.4M | $2.0M |
| 30 | Dementia Alliance of North Carolina INC Raleigh | $1.4M | $1.3M | $1.3M |
| 31 | Heartbright Foundation Charlotte | $1.3M | $1.3M | $262K |
| 32 | Keatons Place Asheboro | $1.2M | $1.2M | $1.6M |
| 33 | Lifecare Memory Partners Wilmington | $1.2M | $1.2M | $110K |
| 34 | Communication Services for the Deaf and Hard of Hearing INC Greensboro | $1.2M | $1.2M | $661K |
| 35 | Village Heartbeat Charlotte | $1.1M | $1.1M | $89K |
| 36 | The Well Project INC a Not for Profit Corporation Raleigh | $1.1M | $1.1M | $280K |
| 37 | Dusty Joy Foundation High Point | $1.1M | $1.1M | $499K |
| 38 | Dup 15Q Alliance Matthews | $1.1M | $988K | $910K |
| 39 | Wards Foundation Charlotte | $1.1M | $955K | $1.4M |
| 40 | Madelyns Fund Charlotte | $1.1M | $828K | $1.4M |
| 41 | Dudleys Place INC Huntersville | $1.1M | $1.1M | $169K |
| 42 | Racing for Als INC Apex | $1.0M | $1.0M | $199K |
| 43 | Diabetes Family Connection Raleigh | $1.0M | $1.0M | $267K |
| 44 | Carolina Breast Friends INC Charlotte | $1.0M | $983K | $1.8M |
| 45 | Diabetessisters Raleigh | $825K | $825K | $788K |
| 46 | CEO Roundtable on Cancer INC Morrisville | $782K | $782K | $2.5M |
| 47 | Alliance of Aids Services-carolina Raleigh | $696K | $696K | $256K |
| 48 | Greensboro Cerebral Palsy Association INC Greensboro | $690K | $667K | $927K |
| 49 | ABC of Nc Foundation Winston Salem | $606K | $501K | $7.5M |
| 50 | National Fabry Disease Foundation Hillsborough | $592K | $592K | $270K |