| 1 | H Lee Moffitt Cancer Center and Research Institute INC Tampa | $542.5M | $314.8M | $2.9B |
| 2 | Can Community Health INC Lakewood Rch | $515.4M | $502.6M | $245.9M |
| 3 | H Lee Moffitt Cancer Center and Research Institute Foundation INC Tampa | $198.5M | $43.8M | $274.2M |
| 4 | Care Resource Community Health Centers INC Miami | $147.5M | $147.4M | $113.7M |
| 5 | H Lee Moffitt Cancer Ctr & Res Inst Life Time Cancer Scrn Ctr INC Tampa | $145.7M | $145.7M | $16.6M |
| 6 | Miami Cancer Institute at Baptist Health INC Coral Gables | $112.9M | $112.9M | $26.6M |
| 7 | Trenton Medical Center INC High Springs | $80.3M | $77.3M | $91.4M |
| 8 | Foundcare INC West Palm Bch | $56.8M | $56.8M | $55.7M |
| 9 | Jacob Zabara Family Foundation Miami Beach | $56.3M | $0 | $59.4M |
| 10 | United Cerebral Palsy of Central Florida INC Orlando | $51.5M | $51.0M | $79.6M |
| 11 | United Cerebral Palsy Association of Miami INC Hialeah | $23.5M | $22.0M | $22.9M |
| 12 | South Florida Public Media Group INC Miami | $21.9M | $13.5M | $43.8M |
| 13 | Els for Autism Foundation Jupiter | $21.3M | $11.4M | $60.7M |
| 14 | Central Florida Kidney Centers INC Orlando | $20.4M | $20.0M | $32.0M |
| 15 | Jewish Family Home Care INC Davie | $16.6M | $16.6M | $6.5M |
| 16 | Copd Foundation INC Coral Gables | $15.9M | $14.4M | $12.5M |
| 17 | Project Response INC Melbourne | $13.8M | $13.8M | $8.8M |
| 18 | Empower U INC Miami | $12.3M | $12.3M | $4.5M |
| 19 | Hemophilia Alliance Group Purchasing Organization INC Clearwater | $10.9M | $10.9M | $26.0M |
| 20 | The Familial Hypercholesterolemia Foundation Fernandina Beach | $8.9M | $8.9M | $7.4M |
| 21 | Positively U INC Winter Haven | $7.8M | $7.8M | $1.6M |
| 22 | Alzheimers Community Care INC West Palm Beach | $6.7M | $6.7M | $5.9M |
| 23 | Global Virus Network INC Tampa | $6.6M | $6.6M | $5.8M |
| 24 | American Otological Societyresearch Foundation St. Petersburg | $6.4M | $869K | $13.1M |
| 25 | Third Wave Volunteers INC Miami | $6.2M | $6.2M | $71K |
| 26 | Memorial Health Systems Foundation INC Daytona Beach | $5.5M | $1.0M | $12.9M |
| 27 | Libbys Legacy Breast Cancer Foundation Orlando | $5.3M | $5.2M | $1.7M |
| 28 | Care 4 U Management Miami | $5.2M | $5.2M | $4.2M |
| 29 | Promise Fund of Florida INC West Palm Beach | $5.2M | $4.2M | $5.1M |
| 30 | Live Like Bella INC Coral Gables | $5.0M | $4.2M | $3.7M |
| 31 | Sunrise Community of Southwest Florida INC Miami | $4.9M | $4.9M | $1.7M |
| 32 | Southeastern Guide Dogs INC Endowment Trust Palmetto | $4.9M | -$33K | $7.8M |
| 33 | Ah of Monroe County INC Key West | $4.9M | $4.8M | $14.8M |
| 34 | Unconditional Love Incorporated Melbourne | $4.8M | $4.8M | $3.2M |
| 35 | Beyond the Spectrum INC Lakewood Rch | $4.6M | $4.6M | $675K |
| 36 | Andrews Research & Education Foundation INC Gulf Breeze | $4.6M | $4.6M | $3.9M |
| 37 | The International Waldenstroms Macroglobulinemia Foundation INC Sarasota | $4.5M | $4.5M | $7.5M |
| 38 | Capstone Adaptive Learning and Therapy Centers INC Pensacola | $4.0M | $4.0M | $5.0M |
| 39 | Who We Play for INC Melbourne | $4.0M | $3.6M | $1.6M |
| 40 | Florida Society of Clinical Oncology INC Riverview | $3.8M | $3.8M | $5.9M |
| 41 | American Uro-gynecologic Society Tampa | $3.7M | $3.7M | $3.9M |
| 42 | Community Access to Child Health of Brevard INC Rockledge | $3.6M | $3.6M | $960K |
| 43 | Conklin Davis Center for the Visually Impaired INC Daytona Beach | $3.5M | $3.5M | $1.8M |
| 44 | Liga Contra el Cancer INC League Against Cancer INC Miami | $3.5M | $2.6M | $7.7M |
| 45 | Parkinson Research Foundation INC Sarasota | $3.2M | $3.2M | $3.7M |
| 46 | Force-facing Our Risk of Cancer Empowered Tampa | $3.2M | $3.0M | $2.1M |
| 47 | Northeast Florida Aids Network INC Jacksonville | $3.0M | $3.0M | $1.3M |
| 48 | Mana-sota Lighthouse for the Blind INC Sarasota | $3.0M | $2.9M | $5.8M |
| 49 | Idiopathic Pulmonary Fibrosis Foundation Estero | $2.9M | $0 | $2.7M |
| 50 | American Academy of Optometry Foundation INC Winter Park | $2.8M | $1.8M | $11.0M |