| 1 | American Society of Hematology INC Washington | $164.7M | $118.7M | $358.4M |
| 2 | American College of Cardiology Foundation Washington | $150.5M | $125.8M | $295.8M |
| 3 | Whitman-walker Clinic INC Washington | $136.8M | $62.4M | $122.0M |
| 4 | Optical Society of America INC Washington | $99.7M | $61.8M | $148.3M |
| 5 | Autism Speaks INC Washington | $37.7M | $35.0M | $35.3M |
| 6 | Endocrine Society Washington | $31.3M | $27.3M | $81.4M |
| 7 | Food & Friends INC Washington | $31.2M | $23.4M | $35.2M |
| 8 | Go2 Foundation for Lung Cancer Washington | $25.3M | $12.2M | $17.2M |
| 9 | Acog Foundation Washington | $22.3M | $13.2M | $138.1M |
| 10 | Melanoma Research Alliance Foundation Washington | $20.6M | $16.5M | $25.2M |
| 11 | Lupus Foundation of America INC Washington | $15.5M | $15.4M | $13.7M |
| 12 | National Minority Aids Council Dba Aids Programs of the National Washington | $13.1M | $9.8M | $15.2M |
| 13 | Pulmonary Hypertension Association INC Washington | $12.6M | $8.5M | $24.7M |
| 14 | Equal Access International Washington | $11.5M | $11.5M | $4.4M |
| 15 | Society for Maternal-fetal Medicine Washington | $10.3M | $9.4M | $14.7M |
| 16 | Childhood Arthritis & Rheumatology Research Alliance Washington | $9.6M | $9.6M | $17.4M |
| 17 | Society of Interventional Oncology INC Washington | $9.1M | $3.8M | $10.1M |
| 18 | Mesothelioma Applied Research Foundation INC Washington | $7.4M | $2.0M | $10.6M |
| 19 | Global Genes Washington | $7.1M | $7.1M | $1.5M |
| 20 | Everylife Foundation for Rare Diseases Washington | $7.0M | $7.0M | $6.2M |
| 21 | Global Alzheimers Platform Foundation INC Washington | $6.5M | $6.5M | $3.5M |
| 22 | Prevention of Blindness Society of Metropolitan Washington INC Washington | $6.1M | $2.8M | $28.2M |
| 23 | Association of Black Cardiologists Washington | $5.3M | $4.9M | $5.9M |
| 24 | Personal Care Products Council Foundation Washington | $4.1M | $3.8M | $2.5M |
| 25 | Personalized Medicine Coalition Washington | $4.1M | $2.8M | $2.3M |
| 26 | The Tiger Lily Foundation INC Washington | $3.1M | $3.1M | $439K |
| 27 | Ias Washington | $2.8M | $2.8M | $11.3M |
| 28 | King Hussein Cancer Foundation USA INC Washington | $2.6M | $2.6M | $1.4M |
| 29 | Art of Living Health and Education Trust Washington | $2.5M | $2.5M | $717K |
| 30 | National Association of Councils on Developmental Disabilities Washington | $2.0M | $2.0M | $1.2M |
| 31 | Kidney Care Partners INC Washington | $1.7M | $1.7M | $2.4M |
| 32 | Autistic Self Advocacy Network Washington | $1.7M | $1.7M | $4.6M |
| 33 | International Cancer Expert Corps INC Washington | $1.5M | $1.5M | $2.3M |
| 34 | Friends of the Global Fight Against Aids Tuberculosis and Malaria Washington | $1.3M | $1.3M | $6.1M |
| 35 | American Association of Pathologists Assistants Washington | $1.2M | $1.2M | $1.1M |
| 36 | American Institute for Medical and Bioligical Engineering Washington | $1.2M | $1.2M | $5.1M |
| 37 | American Academy of Hiv Medicine Foundation Washington | $1.0M | $1.0M | $1.3M |
| 38 | Wishes for Elliott Washington | $914K | $902K | $1.1M |
| 39 | Matthew Harris Ornstein Memorial Foundation INC Washington | $912K | $0 | $2.5M |
| 40 | Global Colon Cancer Association Washington | $888K | $888K | $366K |
| 41 | Adult Vaccine Access Coalition Washington | $820K | $820K | $979K |
| 42 | Council for Quality Respiratory Care Washington | $792K | $792K | $1.1M |
| 43 | Let My Doctors Decide Action Network Washington | $790K | $790K | $165K |
| 44 | Eating Disorders Coalition for Research Policy and Action INC Washington | $665K | $665K | $119K |
| 45 | International Atherosclerosis Society Washington | $570K | $570K | $692K |
| 46 | Nonprofit Kidney Care Alliance Washington | $529K | $529K | $76K |
| 47 | National Forum for Heart Disease Washington | $529K | $529K | $2.0M |
| 48 | Paralyzed Veterans of America Spinal Cord Injury Education and T Washington | $463K | $463K | $872K |
| 49 | Welcoming Neighbors Network Washington | $395K | $395K | $562K |
| 50 | Gail Bleach Vision Research Foundation INC Washington | $353K | $213K | $768K |