| 1 | Museum of Science Boston | $438.9M | $211.9M | $512.8M |
| 2 | Museum of Fine Arts Boston | $425.8M | $157.7M | $1.5B |
| 3 | Harvard Business School Publishing Corporation Brighton | $317.0M | $317.0M | $191.3M |
| 4 | Wgbh Educational Foundation Brighton | $285.1M | $225.0M | $928.3M |
| 5 | Boston Symphony Orchestra INC Boston | $172.0M | $117.1M | $680.3M |
| 6 | Worcester Art Museum Worcester | $101.5M | $39.9M | $181.2M |
| 7 | Peabody Essex Museum INC Salem | $87.3M | $20.0M | $826.0M |
| 8 | Society for the Preservation of New England Antiquities INC Haverhill | $66.1M | $12.1M | $160.8M |
| 9 | Boston Ballet INC Boston | $55.5M | $52.0M | $98.1M |
| 10 | Esplanade Association INC Boston | $37.9M | $9.9M | $23.3M |
| 11 | Trustees of the Berkshire Museum Pittsfield | $34.8M | $5.8M | $78.1M |
| 12 | Wang Theatre INC Boston | $32.9M | $32.8M | $19.9M |
| 13 | Facing History & Ourselves INC Boston | $32.4M | $31.8M | $69.9M |
| 14 | Murray Burnstine Charitable Trust Stoneham | $31.8M | $0 | $17.1M |
| 15 | Holocaust Legacy Foundation INC Boston | $31.3M | $30.2M | $44.1M |
| 16 | Institute of Contemporary Art Boston | $30.9M | $20.4M | $130.4M |
| 17 | Prx Incorporated Brighton | $29.5M | $29.4M | $14.3M |
| 18 | Jacobs Pillow Dance Festival INC Becket | $29.3M | $19.1M | $83.0M |
| 19 | Sterling & Francine Clark Art Inst Williamstown | $28.7M | $27.1M | $691.6M |
| 20 | Scottish Rite Masonic Museum & Library INC Lexington | $27.7M | $1.7M | $57.0M |
| 21 | Dimitris Bertsimas-georgia Perakis Foundation Belmont | $27.1M | $0 | $69.9M |
| 22 | American Repertory Theatre Cambridge | $26.7M | $25.9M | $32.3M |
| 23 | The Walden Woods Project Lincoln | $26.3M | $8.0M | $46.7M |
| 24 | Isabella Stewart Gardner Museum INC Boston | $23.6M | $22.3M | $331.6M |
| 25 | Technology Review INC Cambridge | $22.3M | $22.3M | $6.5M |
| 26 | Historic Deerfield INC Deerfield | $22.1M | $16.7M | $86.2M |
| 27 | New England Foundation for the Arts INC Boston | $21.6M | $19.2M | $42.4M |
| 28 | Old Dartmouth Historical Society New Bedford | $21.2M | $18.7M | $51.3M |
| 29 | Mapping Boston Foundation INC Boston | $20.9M | $0 | $18.7M |
| 30 | The Hyams Foundation INC Boston | $20.0M | $0 | $144.3M |
| 31 | Eastern Educational Television Network INC Boston | $19.6M | $17.1M | $22.5M |
| 32 | Nantucket Historical Association Nantucket | $19.2M | $14.9M | $55.5M |
| 33 | Huntington Theatre Company INC Boston | $18.0M | $17.8M | $123.8M |
| 34 | Cape Ann Museum INC Gloucester | $16.6M | $11.5M | $38.5M |
| 35 | Plimoth Patuxet Museums INC Plymouth | $16.4M | $9.6M | $40.1M |
| 36 | Groton Hill Music INC Groton | $16.3M | $13.6M | $248.6M |
| 37 | The Childrens Museum Boston | $16.0M | $11.9M | $64.9M |
| 38 | Worcester Center for Performing Arts INC Worcester | $15.3M | $15.3M | $11.7M |
| 39 | Aurora Humanitarian Initiative Foundation INC Cambridge | $15.3M | $15.3M | $16.8M |
| 40 | Heritage Plantation of Sandwich INC Sandwich | $15.1M | $13.4M | $46.9M |
| 41 | Boston Lyric Opera Company INC Boston | $15.0M | $10.1M | $35.5M |
| 42 | Newport Festivals Foundation INC Manchester | $14.8M | $12.5M | $26.7M |
| 43 | National Yiddish Book Center INC Amherst | $14.7M | $14.4M | $89.1M |
| 44 | South Shore Playhouse Associates INC Cohasset | $13.1M | $9.2M | $20.9M |
| 45 | Center for Independent Documentary INC Cambridge | $13.0M | $13.0M | $8.9M |
| 46 | The Wang Center for the Performing Arts INC Boston | $12.9M | $8.8M | $40.7M |
| 47 | Boston Youth Symphony Orchestra INC Boston | $12.4M | $5.6M | $30.7M |
| 48 | Eric Carle Museum of Picture Book Art INC Amherst | $12.3M | $6.6M | $20.9M |
| 49 | Plum Bush Foundation Millis | $12.1M | $0 | $16.3M |
| 50 | Old Sturbridge INC Sturbridge | $12.1M | $9.0M | $30.0M |