| 1 | Field Museum of Natural History Chicago | $698.3M | $114.8M | $932.9M |
| 2 | The Barack Obama Foundation Chicago | $619.4M | $209.8M | $1.1B |
| 3 | Chicago Symphony Orchestra Chicago | $369.5M | $125.5M | $581.8M |
| 4 | Kenneth C Griffin Museum of Science and Industry Chicago | $285.4M | $66.4M | $433.0M |
| 5 | Museum of Contemporary Art Chicago | $169.1M | $17.3M | $210.4M |
| 6 | Lyric Opera of Chicago Chicago | $139.8M | $57.1M | $266.3M |
| 7 | Chicago Theatre Group INC Chicago | $103.7M | $29.4M | $55.1M |
| 8 | The Poetry Foundation Chicago | $101.3M | $0 | $313.2M |
| 9 | Navy Pier INC Chicago | $87.2M | $74.6M | $183.2M |
| 10 | Ravinia Festival Association Highland Park | $85.3M | $66.2M | $275.1M |
| 11 | Window to the World Communications INC Chicago | $48.3M | $40.1M | $92.9M |
| 12 | Adler Planetarium Chicago | $46.2M | $22.0M | $72.6M |
| 13 | Chicago Public Media INC Chicago | $45.0M | $35.6M | $123.1M |
| 14 | Steppenwolf Theater Co Chicago | $37.4M | $25.6M | $111.4M |
| 15 | National Board of Osteopathic Medical Examiners INC Chicago | $36.4M | $34.1M | $49.8M |
| 16 | Radiant Life Ministries Marion | $36.1M | $36.1M | $103.2M |
| 17 | Holocaust Memorial Foundation of Illinois INC Skokie | $33.4M | $12.5M | $85.3M |
| 18 | The Joffrey Ballet Chicago | $30.3M | $28.5M | $52.4M |
| 19 | Chicago Historical Society Chicago | $26.0M | $13.4M | $126.0M |
| 20 | The Richard H Driehaus Museum Chicago | $23.2M | $5.9M | $123.4M |
| 21 | Auditorium Theatre of Roosevelt University INC Chicago | $21.6M | $20.2M | $17.8M |
| 22 | Lithuanian Foundation Lemont | $20.3M | $5.9M | $59.0M |
| 23 | United States Artists INC Chicago | $18.8M | $9.6M | $64.3M |
| 24 | Old Town School of Folk Music INC Chicago | $17.2M | $14.0M | $26.7M |
| 25 | Mariners Museum Tr No 2 P03152009 Chicago | $15.9M | $3.4M | $45.3M |
| 26 | Chicago Architecture Foundation Chicago | $15.8M | $14.7M | $27.5M |
| 27 | Timeline Theatre Company Chicago | $15.5M | $15.4M | $27.0M |
| 28 | Music and Dance Theater Chicago INC Chicago | $14.6M | $8.9M | $57.3M |
| 29 | Chicago Shakespeare Theater Chicago | $14.1M | $13.1M | $51.8M |
| 30 | National Museum of Mexican Art Chicago | $14.1M | $8.0M | $25.2M |
| 31 | Merit School of Music Chicago | $13.5M | $16.0M | $43.0M |
| 32 | Grant Park Orchestral Association Chicago | $13.3M | $13.1M | $30.8M |
| 33 | Northlight Theatre INC Skokie | $12.2M | $12.0M | $31.0M |
| 34 | The Historymakers Chicago | $11.9M | $6.7M | $17.1M |
| 35 | Chicago Police Memorial Foundation Chicago | $11.9M | $6.2M | $24.7M |
| 36 | Uniting Voices Chicago | $10.8M | $6.2M | $13.5M |
| 37 | Mariners Museum Tr No 1 P03583005 Chicago | $10.8M | $1.9M | $29.8M |
| 38 | Chicago Childrens Museum Chicago | $10.6M | $10.0M | $17.8M |
| 39 | The Center for Economic Research and Social Change INC Chicago | $10.3M | $9.2M | $18.4M |
| 40 | Music Institute of Chicago Evanston | $10.2M | $7.1M | $10.8M |
| 41 | Chicago Academy of Sciences Chicago | $9.2M | $7.3M | $23.1M |
| 42 | Writers Theatre INC Glencoe | $9.0M | $7.4M | $32.9M |
| 43 | Robert Allerton Trust Under Will Dated April 15 1964 Chicago | $9.0M | $0 | $36.8M |
| 44 | Friends of the Marthas Vineyard Concert Series Chicago | $8.7M | $8.7M | $1.4M |
| 45 | The Christian Century Chicago | $8.6M | $7.2M | $21.4M |
| 46 | Klairmont Kollections Nfp Chicago | $8.1M | $0 | $24.7M |
| 47 | Chicago Humanities Festival Chicago | $7.4M | $4.2M | $20.6M |
| 48 | American College of Occupational and Environmental Medicine Schaumburg | $7.2M | $6.8M | $4.4M |
| 49 | Winnetka Community House Winnetka | $7.1M | $5.7M | $21.4M |
| 50 | Frank Lloyd Wright Trust Oak Park | $6.9M | $6.2M | $12.5M |