| 1 | Houston Zoo INC Houston | $99.7M | $82.7M | $380.8M |
| 2 | Fort Worth Zoological Association INC Fort Worth | $90.5M | $52.8M | $117.2M |
| 3 | Rewild Austin | $75.0M | $74.2M | $84.0M |
| 4 | San Antonio Zoological Society INC San Antonio | $71.5M | $61.1M | $138.6M |
| 5 | American Quarter Horse Foundation Amarillo | $48.3M | $11.9M | $71.9M |
| 6 | Houston Society for the Prevention of Cruelty to Animals Houston | $41.4M | $21.4M | $78.1M |
| 7 | Dallas Zoo Management INC Dallas | $34.1M | $34.1M | $5.7M |
| 8 | Emancipet Austin | $30.6M | $26.2M | $12.0M |
| 9 | Operation Kindness Carrollton | $27.3M | $18.6M | $28.5M |
| 10 | Safari Club International Foundatio San Antonio | $26.6M | $16.0M | $48.9M |
| 11 | Houston Humane Society Houston | $26.0M | $12.4M | $27.7M |
| 12 | SPCA of Texas Dallas | $25.8M | $18.7M | $43.8M |
| 13 | Dallas Zoological Society Dallas | $25.3M | $24.3M | $43.6M |
| 14 | Austin Pets Alive Austin | $24.5M | $21.7M | $27.1M |
| 15 | Spay Neuter Network Crandall | $20.6M | $7.3M | $13.4M |
| 16 | Rodeo Austin Austin | $19.5M | $17.1M | $22.3M |
| 17 | Texas State Aquarium Association Corp Christi | $19.2M | $19.1M | $93.7M |
| 18 | Peaceful Valley Donkey Rescue INC Miles | $15.8M | $15.7M | $10.8M |
| 19 | Zoo Midland INC Midland | $13.4M | $13.4M | $12.2M |
| 20 | Valley Zoological Society Chartered May 1968 Brownsville | $12.1M | $9.5M | $14.4M |
| 21 | Gulf Coast Humane Society Corp Christi | $11.4M | $11.4M | $12.1M |
| 22 | Wildlife Rescue and Rehabilitation INC Kendalia | $10.7M | $10.7M | $21.4M |
| 23 | Animal Defense League San Antonio | $10.3M | $10.2M | $13.3M |
| 24 | Humane Society of North Texas Fort Worth | $8.3M | $6.5M | $14.5M |
| 25 | Veterinary Emergency and Critical Care Society San Antonio | $7.9M | $7.6M | $14.9M |
| 26 | Animal Defense League Foundation San Antonio | $7.9M | $657K | $25.6M |
| 27 | All Kind Animal Initiative Abilene | $7.6M | $7.5M | $13.4M |
| 28 | Humane Society of San Antonio San Antonio | $7.2M | $7.2M | $24.7M |
| 29 | Citizens for Animal Protection INC Houston | $7.2M | $3.6M | $10.7M |
| 30 | The International Rhino Foundation Fort Worth | $6.8M | $3.0M | $2.0M |
| 31 | Girtys Place Animal Rescue and Sanctuary Farmersville | $6.6M | $6.6M | $994K |
| 32 | Elephant Havens Wildlife Foundation INC Dallas | $6.1M | $5.4M | $3.6M |
| 33 | Texas Litter Control Spring | $6.0M | $6.0M | $923K |
| 34 | Pets Low Cost Spay and Neuter Clinic Wichita Falls | $6.0M | $6.0M | $4.8M |
| 35 | Humane Society of Austin & Travis County Austin | $5.9M | $5.5M | $15.5M |
| 36 | Hopkins County Animal Protection League INC Sulphur Spgs | $5.4M | $5.4M | $793K |
| 37 | Spay-neuter Assistance Program INC Houston | $5.2M | $5.2M | $1.4M |
| 38 | Abilene Zoological Society Abilene | $5.0M | $4.3M | $732K |
| 39 | Fred and Armour Shin Foundation Burnet | $4.8M | $0 | $10.6M |
| 40 | Grover Nelson Park Zoological Foundation Tuscola | $4.5M | $4.4M | $9.1M |
| 41 | San Antonio Pets Alive INC San Antonio | $4.4M | $4.4M | $5.5M |
| 42 | Three Little Pitties Rescue Friendswood | $4.4M | $4.3M | $1.8M |
| 43 | James L West Presbyterian Special Care Center Foundation Fort Worth | $4.4M | $3.2M | $8.5M |
| 44 | Habitat for Horses INC Alvin | $4.3M | $2.5M | $4.1M |
| 45 | Wildlife Association of Texas New Braunfels | $4.2M | $3.8M | $2.2M |
| 46 | Aggieland Humane Society Bryan | $4.1M | $2.6M | $8.0M |
| 47 | Rescued Pets Movement INC Houston | $4.1M | $4.1M | $3.1M |
| 48 | Palm Valley Animal Center Edinburg | $4.0M | $3.9M | $3.6M |
| 49 | Westchester Foundation San Antonio | $3.9M | $0 | $6.8M |
| 50 | Texas Animal Damage Control Association Cooperative Trapping F San Antonio | $3.8M | $3.8M | $3.9M |