| 1 | Tennessee Wildlife Resources Foundation INC Nashville | $61.8M | $19.8M | $14.4M |
| 2 | Nashville Zoo INC Nashville | $42.3M | $35.9M | $182.7M |
| 3 | Tennessee Aquarium Chattanooga | $40.6M | $34.2M | $92.5M |
| 4 | Memphis Zoo INC Memphis | $28.3M | $25.8M | $9.0M |
| 5 | Knoxville Zoological Gardens INC Knoxville | $17.1M | $16.5M | $64.7M |
| 6 | The Elephant Sanctuary in Tennessee Hohenwald | $17.0M | $9.4M | $100.4M |
| 7 | Memphis Zoological Foundation Collierville | $9.8M | $922K | $9.2M |
| 8 | Humane Society of Memphis & Shelby County Memphis | $9.3M | $4.6M | $11.8M |
| 9 | Young-williams Animal Center of East Tennessee Knoxville | $8.6M | $8.5M | $8.4M |
| 10 | Nashville Humane Association Supporting Foundation Nashville | $8.5M | $798K | $37.5M |
| 11 | Old Friends Senior Dogs Mount Juliet | $8.1M | $2.5M | $17.9M |
| 12 | Tiger Haven Kingston | $8.1M | $5.6M | $14.1M |
| 13 | American Fisheries Society INC Nashville | $7.2M | $6.1M | $7.0M |
| 14 | Friends of the Zoo INC Chattanooga | $6.8M | $6.8M | $2.3M |
| 15 | American Association for Laboratory Animal Science Aalas Memphis | $6.5M | $5.3M | $17.3M |
| 16 | Project K9 Hero Whitwell | $6.1M | $5.9M | $3.1M |
| 17 | Nashville Humane Association Nashville | $4.5M | $4.3M | $6.1M |
| 18 | Animal Care Tr Chattanooga | $3.8M | $3.7M | $5.2M |
| 19 | Global Sanctuary for Elephants Brentwood | $3.6M | $3.6M | $2.5M |
| 20 | Wildlife Society INC Nashville | $3.5M | $3.1M | $5.5M |
| 21 | Horse Plus Humane Society Hohenwald | $3.2M | $3.2M | $2.7M |
| 22 | Muttnation Foundation INC Nashville | $2.9M | $2.8M | $3.1M |
| 23 | Humane Educational Society of Chattanooga Tenn Chattanooga | $2.5M | $2.3M | $10.6M |
| 24 | Sharon Charitable Tr Savannah | $2.4M | $0 | $12.9M |
| 25 | Pet Community Center INC Nashville | $2.3M | $2.3M | $1.6M |
| 26 | Washington County-johnson City Animal Control Center INC Johnson City | $2.1M | $2.1M | $6.0M |
| 27 | Kabul Small Animal Rescue Oliver Spgs | $1.9M | $1.9M | $58K |
| 28 | Monroe County Friends of Animals Madisonville | $1.8M | $1.4M | $6.3M |
| 29 | The Pet Compassion Centers Memphis | $1.6M | $1.6M | $505K |
| 30 | Humane Society of the Tennessee Valley INC Knoxville | $1.5M | $1.5M | $2.8M |
| 31 | Blood Origins INC Collierville | $1.4M | $1.4M | $993K |
| 32 | People Promoting Animal Welfare Lenoir City | $1.4M | $1.4M | $1.8M |
| 33 | Big Fluffy Dog Rescue La Vergne | $1.2M | $1.2M | $78K |
| 34 | Proverbs 12 10 Animal Rescue & Adoption Burns | $1.2M | $1.2M | $430K |
| 35 | American Eagle Foundation Kodak | $1.1M | $1.1M | $10.0M |
| 36 | Appalachian Black Bear Rehabilitation and Release Center Townsend | $1.1M | $1.0M | $1.1M |
| 37 | The Humane Association of Wilson Lebanon | $1.1M | $1.1M | $2.4M |
| 38 | Jesse C Beesley Animal Humane Foundation INC Murfreesboro | $1.0M | $993K | $2.1M |
| 39 | Tennessee Reining Horse Association Speedwell | $944K | $944K | $121K |
| 40 | Petworks Animal Services INC Kingsport | $943K | $920K | $3.9M |
| 41 | True Rescue Mount Juliet | $926K | $923K | $422K |
| 42 | Chattaneuter Chattanooga | $896K | $894K | $623K |
| 43 | McMinn Regional Humane Society Athens | $867K | $859K | $917K |
| 44 | Odd Man Inn Animal Refuge Jamestown | $828K | $828K | $1.5M |
| 45 | Companion Pet Rescue and Transport of W Tn INC Jackson | $821K | $821K | $199K |
| 46 | Greeneville-green County Humane Society INC Greeneville | $805K | $781K | $8.1M |
| 47 | Sevier County Animal Shelter Sevierville | $769K | $767K | $167K |
| 48 | Sumner Spay Neuter Alliance Gallatin | $751K | $736K | $1.3M |
| 49 | American Holistic Veterinary Medical Association Fayetteville | $733K | $733K | $366K |
| 50 | Humane Society of Dickson County Dickson | $711K | $707K | $2.7M |