| 1 | The North Carolina Aquarium Society Raleigh | $24.2M | $16.8M | $49.2M |
| 2 | North Carolina Zoological Society INC Asheboro | $17.2M | $14.5M | $66.7M |
| 3 | Brother Wolf Animal Rescue INC Asheville | $15.0M | $14.7M | $15.5M |
| 4 | American Kennel Club Canine Health Foundation INC Raleigh | $12.0M | $6.4M | $38.2M |
| 5 | Society for the Prevention of Cruelty to Animals Wake County INC Raleigh | $11.9M | $11.6M | $23.2M |
| 6 | Wysocking Wildlife Sanctuary INC Engelhard | $8.4M | $0 | $18.4M |
| 7 | Humane Society of Charlotte INC Charlotte | $7.2M | $7.0M | $25.4M |
| 8 | Asheville Humane Society INC Asheville | $6.5M | $5.8M | $4.8M |
| 9 | Spay Neuter Charlotte Charlotte | $5.8M | $5.7M | $5.8M |
| 10 | Blue Ridge Humane Society INC Hendersonvlle | $4.6M | $3.5M | $6.9M |
| 11 | Forsyth Humane Society Winston Salem | $3.3M | $3.1M | $6.9M |
| 12 | Animal Protection Society of Durham Durham | $3.2M | $2.6M | $2.0M |
| 13 | Carolina Tiger Rescue Pittsboro | $3.2M | $3.0M | $5.4M |
| 14 | Animal League of Gaston County Gastonia | $3.0M | $1.9M | $3.4M |
| 15 | North Carolina Wildlife Habitat Foundation INC Wilmington | $2.9M | $1.0M | $8.0M |
| 16 | Saving Grace Animals for Adoption INC Wake Forest | $2.6M | $2.6M | $1.7M |
| 17 | Karen Beasley Sea Turtle Rescue and Rehabilitation Center Surf City | $2.2M | $1.8M | $7.9M |
| 18 | Blind Cat Rescue & Sanctuary INC Saint Pauls | $2.2M | $1.6M | $5.7M |
| 19 | Ncasi Foundation Cary | $2.1M | $2.1M | $1.2M |
| 20 | Humane Society of Catawba County Hickory | $2.1M | $2.1M | $2.5M |
| 21 | Humane Society of the Piedmont INC Greensboro | $2.1M | $2.0M | $1.6M |
| 22 | Red Bell Run Foundation INC Columbus | $1.9M | $1.9M | $293K |
| 23 | Sylvan Heights Waterfowl Park and Eco-center INC Scotland Neck | $1.9M | $1.8M | $4.2M |
| 24 | Second Chance Pet Adoptions INC Raleigh | $1.9M | $1.8M | $2.2M |
| 25 | Grey Muzzle Organization Raleigh | $1.8M | $1.8M | $2.9M |
| 26 | Carolina Raptor Center INC Huntersville | $1.7M | $1.7M | $2.0M |
| 27 | Humane Society of Union County INC Monroe | $1.7M | $1.7M | $1.2M |
| 28 | Renaissance Equine Rescue & Welfare Foundation Vass | $1.6M | $0 | $7.2M |
| 29 | Friends of Bonobos Durham | $1.5M | $1.5M | $449K |
| 30 | Cabarrus Spay & Neuter Clinic INC Concord | $1.5M | $1.5M | $1.5M |
| 31 | Valley River Humane Society INC Murphy | $1.5M | $1.5M | $860K |
| 32 | Safe Haven for Cats Raleigh | $1.5M | $1.4M | $1.3M |
| 33 | Lenoir County SPCA INC Kinston | $1.4M | $1.1M | $6.5M |
| 34 | Red Dog Farm Animal Rescue Network Stokesdale | $1.4M | $764K | $2.5M |
| 35 | Humane Society of Moore County INC Southern Pnes | $1.3M | $1.3M | $4.8M |
| 36 | Conservation Through Public Health Holly Springs | $1.3M | $1.3M | $788K |
| 37 | Corolla Wild Horse Fund INC Corolla | $1.2M | $1.2M | $2.4M |
| 38 | Forgotten Now Family Rescue Co Cornelius | $1.2M | $1.2M | $104K |
| 39 | Lake Norman Humane INC Mooresville | $1.2M | $1.2M | $3.4M |
| 40 | Wing Haven Foundation Incorporated Charlotte | $1.1M | $1.1M | $14.1M |
| 41 | Archdale Affordable Neuter & Wellness Clinic INC Archdale | $1.1M | $780K | $163K |
| 42 | Possumwood Acres Wildlife Sanctuary Hubert | $1.1M | $1.1M | $1.1M |
| 43 | Cashiers-highlands Humane Society INC Cashiers | $1.1M | $1.0M | $4.3M |
| 44 | China Rescue Dogs INC Vass | $1.1M | $1.1M | $206K |
| 45 | Ziggys Refuge Providence | $1.1M | $1.1M | $1.6M |
| 46 | Yancey County Humane Society Burnsville | $1.0M | $776K | $1.3M |
| 47 | Adopt-an-angel Wilmington | $1.0M | $1.0M | $1.3M |
| 48 | North Mecklenburg Animal Rescue INC Concord | $1.0M | $1.0M | $1.4M |
| 49 | Watauga Humane Society Boone | $1.0M | $997K | $5.0M |
| 50 | Animal Protection Society of Orange Co INC Mebane | $994K | $913K | $1.1M |