| 1 | Johnny Morris Ozarks Heritage Preserve Springfield | $170.0M | $168.0M | $516.2M |
| 2 | The Saint Louis Zoo Foundation Saint Louis | $62.4M | $58.5M | $303.7M |
| 3 | Friends of the Zoo INC of Kansas City Mo Kansas City | $57.3M | $53.6M | $37.1M |
| 4 | Turtle Beach Wildlife Reserve Saint Louis | $45.8M | $0 | $8.3M |
| 5 | Humane Society of Missouri Saint Louis | $30.1M | $27.2M | $135.7M |
| 6 | Animal Protective Association of Missouri Saint Louis | $22.4M | $14.8M | $34.1M |
| 7 | Wayside Waifs INC Kansas City | $17.2M | $10.3M | $40.9M |
| 8 | Kansas City Pet Project Kansas City | $10.8M | $10.4M | $3.5M |
| 9 | Central Missouri Humane Society Columbia | $8.2M | $4.4M | $6.9M |
| 10 | Stray Rescue of St. Louis Saint Louis | $7.4M | $7.2M | $8.9M |
| 11 | Dogs on Duty Saint Charles | $6.1M | $6.1M | $813K |
| 12 | Spay and Neuter Kansas City Kansas City | $5.3M | $5.3M | $1.4M |
| 13 | Southwest Missouri Humane Society Springfield | $4.0M | $2.6M | $9.3M |
| 14 | Kansas City Campus for Animal Care Kansas City | $3.8M | $3.8M | $3.2M |
| 15 | Zoo Learning Fund Kansas City | $3.5M | $602K | $5.5M |
| 16 | Friends of the Zoo of Springfield Missouri INC Springfield | $2.8M | $2.4M | $1.6M |
| 17 | Retrieving Freedom Sedalia | $2.5M | $2.5M | $5.2M |
| 18 | Carol a Strother Foundation Saint Louis | $2.5M | $0 | $6.1M |
| 19 | St. Louis Metropolitan Spay Neuter & Wellness Clinic INC Saint Louis | $2.4M | $2.4M | $2.0M |
| 20 | World Bird Sanctuary Valley Park | $2.3M | $1.8M | $1.9M |
| 21 | Joplin Humane Society INC Joplin | $2.3M | $2.1M | $7.6M |
| 22 | Five Acres Animal Shelter Saint Charles | $2.1M | $1.6M | $3.3M |
| 23 | M Shoogy Animal Rescue Savannah | $2.0M | $1.4M | $4.6M |
| 24 | Wild Canid Survival & Research Center INC Eureka | $1.9M | $1.9M | $5.3M |
| 25 | Humane Society of Southeast Mo Cpe Girardeau | $1.9M | $1.9M | $4.3M |
| 26 | Center for Animal Rescue & Enrichment of St. Louis Saint Louis | $1.8M | $1.7M | $926K |
| 27 | Rescue One Springfield | $1.6M | $1.5M | $1.4M |
| 28 | Dreamchaser Pmu Horse Rescue and Rehabilitation INC Falcon | $1.5M | $1.5M | $1.1M |
| 29 | Clementine Foundation Created Uta 12032015 as Amended Kansas City | $1.5M | $0 | $15.4M |
| 30 | Jennifer Alaine Willett Charitable Foundation INC Dardenne Pr | $1.4M | $0 | $1.3M |
| 31 | Global Resources for Environmental Education and Nature Saddlebrooke | $1.3M | $1.3M | $2.7M |
| 32 | Missouri Forget-me-not Horse Rescue and Sanctuary Linn Creek | $1.3M | $1.3M | $3.2M |
| 33 | Friends of the Animal Shelter of Saint Joseph INC Saint Joseph | $1.2M | $632K | $2.4M |
| 34 | Whispering Willows Senior Dog Sanctuary INC Hermitage | $1.1M | $1.1M | $75K |
| 35 | Dogwood Animal Shelter INC Osage Beach | $1.1M | $1.1M | $1.8M |
| 36 | Paisley Paws INC Kearney | $1.1M | $1.1M | $943K |
| 37 | Varlebena Farms Foundation Elsberry | $1.0M | $1.0M | $917K |
| 38 | Help of Raymore INC Belton | $1.0M | $1.0M | $646K |
| 39 | Friends of the Zoo of Kansas City Foundation Kansas City | $967K | $759K | $5.9M |
| 40 | Faithful Friends Animal Advocates INC Neosho | $929K | $649K | $741K |
| 41 | Old Drum Animal Shelter Warrensburg | $854K | $850K | $352K |
| 42 | Pawportunities Blue Springs | $806K | $804K | $187K |
| 43 | Missouri Wildlife Rescue Center Ballwin | $789K | $773K | $1.7M |
| 44 | St. Louis Aquarium at Union Station Foundation St. Louis | $786K | $580K | $2.0M |
| 45 | Springfield Animal Advocacy Foundation Springfield | $769K | $769K | $366K |
| 46 | Daniel J Reid Foundation Saint Louis | $756K | $0 | $2.3M |
| 47 | Franklin County Humane Society of Missouri Union | $749K | $737K | $875K |
| 48 | Havaheart Rescue Springfield | $721K | $721K | $131K |
| 49 | American Black Hereford Assoc INC Jefferson Cty | $701K | $701K | $288K |
| 50 | Pets a Lone Sanctuary of Lincoln Troy | $627K | $627K | $681K |