| 1 | Ahimsa Foundation Niwot | $399.3M | $0 | $1.0B |
| 2 | Denver Dumb Friends League Denver | $131.3M | $92.9M | $180.3M |
| 3 | Denver Zoological Foundation INC Denver | $60.4M | $60.1M | $32.6M |
| 4 | Cheyenne Mountain Zoological Society Colorado Springs | $42.8M | $42.8M | $135.6M |
| 5 | The Wild Animal Sanctuary a Colorado Non-profit Corporation Keenesburg | $36.8M | $36.4M | $51.2M |
| 6 | Karuna Foundation Niwot | $35.4M | $35.4M | $2.8M |
| 7 | Humane Society of the Pikes Peak Region Colorado Spgs | $27.7M | $21.5M | $30.2M |
| 8 | Noco Humane Loveland | $20.4M | $11.2M | $33.5M |
| 9 | Morris Animal Foundation Denver | $14.4M | $14.4M | $112.2M |
| 10 | The Humane Society of Boulder Valley INC Boulder | $10.4M | $9.5M | $19.4M |
| 11 | Rocky Mountain Bird Observatory Brighton | $10.2M | $10.1M | $5.7M |
| 12 | Wildlife Protection Solutions Golden | $9.2M | $3.3M | $19.4M |
| 13 | Rocky Mountain Butterfly Consortium Westminster | $9.2M | $8.2M | $9.7M |
| 14 | The Maxfund INC Denver | $7.3M | $7.2M | $12.6M |
| 15 | Environment for the Americas INC Boulder | $7.1M | $7.0M | $944K |
| 16 | Colorado Horse Rescue Longmont | $6.6M | $1.9M | $5.5M |
| 17 | Longmont Humane Society INC Longmont | $5.4M | $5.3M | $7.5M |
| 18 | Native American Fish & Wildlife Society Northglenn | $5.2M | $5.2M | $8.5M |
| 19 | Sally Judith McLellan Wildlife Foundation Littleton | $5.1M | $0 | $5.1M |
| 20 | Animal Friends Alliance Fort Collins | $5.1M | $5.0M | $6.2M |
| 21 | La Plata County Humane Society Durango | $4.0M | $3.2M | $4.3M |
| 22 | Colorado Pet Pantry Boulder | $3.6M | $3.5M | $1.3M |
| 23 | Cat Care Society Lakewood | $3.6M | $3.3M | $4.7M |
| 24 | Planned Pethood International Conifer | $3.5M | $3.1M | $3.9M |
| 25 | Pueblo Zoological Society Pueblo | $3.1M | $2.9M | $5.1M |
| 26 | Dogs Playing for Life Longmont | $2.7M | $2.7M | $1.2M |
| 27 | Moms and Mutts Colorado Rescue for Pregnant and Nursing Dogs Littleton | $2.5M | $2.5M | $327K |
| 28 | Colorado Wolf and Wildlife Center Divide | $2.5M | $2.4M | $9.9M |
| 29 | Colorado Zoological Trust INC Denver | $2.5M | $2.5M | $33.2M |
| 30 | Friends of Foothills Animal Shelter Golden | $2.1M | $2.1M | $104K |
| 31 | American Birding Association INC Colorado Spgs | $2.0M | $2.0M | $573K |
| 32 | Second Chance Humane Society INC Ridgway | $2.0M | $2.0M | $2.8M |
| 33 | Pet Sustainability Coalition Boulder | $2.0M | $2.0M | $478K |
| 34 | Soul Dog Rescue Fort Lupton | $1.9M | $1.9M | $1.0M |
| 35 | Greenwood Wildlife Rehabilitation Center Boulder | $1.9M | $1.7M | $2.7M |
| 36 | Golden Retriever Rescue of the Rockies Arvada | $1.8M | $1.2M | $4.8M |
| 37 | Hamlett Spay and Neuter Clinic of Colorado Springs Colorado Spgs | $1.7M | $1.7M | $1.2M |
| 38 | South Platte Water Related Activities Program INC Sterling | $1.7M | $1.7M | $6.8M |
| 39 | Trout Unlimited Denver | $1.5M | $1.4M | $1.6M |
| 40 | Luvin Arms Erie | $1.5M | $1.5M | $3.8M |
| 41 | Street Dog Coalition Fort Collins | $1.5M | $1.5M | $874K |
| 42 | Humane Society of Pagosa Springs INC Pagosa Spgs | $1.5M | $1.3M | $2.4M |
| 43 | Animal Welfare and Protection Society Pueblo | $1.5M | $1.3M | $3.9M |
| 44 | Spaytoday Lakewood | $1.5M | $1.4M | $823K |
| 45 | Humane Society of the South Platte Valley INC Littleton | $1.4M | $1.4M | $1.6M |
| 46 | Roice-hurst Humane Society INC Grand Jct | $1.4M | $1.3M | $3.7M |
| 47 | Big Bones Canine Rescue Service Windsor | $1.3M | $1.3M | $178K |
| 48 | Journey Home Animal Center INC Rifle | $1.3M | $1.2M | $2.8M |
| 49 | Blue River Horse Center INC Silverthorne | $1.2M | $705K | $980K |
| 50 | Lifeline Pet Rescue Brighton | $1.2M | $1.2M | $539K |